中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (12): 2191-2196.doi: 10.3969/j.issn.2095-4344.2013.12.015

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

乙酰化可降低聚酰胺-胺的细胞毒性

刘鉴峰,褚丽萍,王德芝,贺 欣,徐宏艳,刘金剑   

  1. 中国医学科学院放射医学研究所,天津市分子核医学重点实验室,天津市 300192
  • 收稿日期:2012-07-14 修回日期:2012-08-14 出版日期:2013-03-19 发布日期:2013-03-19
  • 通讯作者: 刘金剑,中国医学科学院放射医学研究所,天津市分子核医学重点实验室,天津市 300192 liujinjian2002@163.com
  • 作者简介:刘鉴峰☆,男,1979年生,山东省胶南市人,汉族,2011年南开大学生命科学学院毕业,博士,主要从事靶向药物载体方面的研究。 lewis78@163.com

Acetylation attenuates polyamidoamine cytotoxicity

Acetylation attenuates polyamidoamine cytotoxicity   

  1. Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China
  • Received:2012-07-14 Revised:2012-08-14 Online:2013-03-19 Published:2013-03-19
  • Contact: Liu Jin-jian, Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China liujinjian2002@163.com
  • About author:Liu Jian-feng☆, Doctor, Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China lewis78@163.com

摘要:

背景:聚酰胺-胺型树枝状高分子纳米材料已被广泛应用于药物载体的研究,但由于整代聚酰胺-胺表面有大量带正电荷的氨基,具有一定的细胞毒性。
目的:观察乙酰化对聚酰胺-胺细胞毒性的影响。
方法:①细胞增殖检测:采用MTT法检测在含0,0.125,0.25,0.5,1,2,4 μmol/L乙酰化聚酰胺-胺的培养液中人胚肾293T细胞的增殖。②细胞形态:倒置荧光显微镜观察在含4 μmol/L乙酰化聚酰胺-胺的培养液中人胚肾293T细胞的形态。③细胞周期:流式细胞术检测在含0,5,10,15,20 mg/L乙酰化聚酰胺-胺的培养液中人胚肾293T细胞的细胞周期。
结果与结论:聚酰胺-胺对293T细胞具有一定的细胞毒性,在4 μmol/L浓度下48 h的细胞存活率仅为52%,而乙酰化可显著降低聚酰胺-胺的细胞毒性(P < 0.01); 聚酰胺-胺孵育的细胞发生团缩,伸展性变差,而乙酰化聚酰胺-胺孵育的293T细胞与正常培养细胞基本一致,具有良好的伸展性;乙酰化聚酰胺-胺对细胞周期无影响,而聚酰胺-胺在20 mg/L较高质量浓度时可使细胞S期产生阻滞。表明乙酰化可以降低聚酰胺-胺的细胞毒性。

关键词: 生物材料, 材料生物相容性, 聚酰胺-胺, 乙酰化, 细胞毒性, 293T细胞, 增殖, 细胞周期, 国家自然科学基金, 生物材料图片文章

Abstract:

BACKGROUND: Polyamidoamine dendrimer nanomaterials have been widely used in drug carrier research, but there are many electropositive amino groups on the surface of the entire generation polyamidoamine, resulting in certain cytotoxicity.
OBJECTIVE: To study the influence of acetylation on polyamidoamine cytotoxicity.
METHODS: (1) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay: the cell proliferation of 293T cells incubated with acetylated polyamidoamine under 0, 0.125, 0.25, 0.5, 1, 2, 4 μmol/L concentrations was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. (2) Cell morphology: the cell morphology of 293T cells incubated with 4 μmol/L acetylated polyamidoamine was observed by inverted fluorescence microscope. (3) Cell cycle: the cell cycle of 293T cells incubated with acetylatedpolyamidoamine under 0, 5, 10, 15, 20 mg/L concentrations was detected by flow cytometry.
RESULTS AND CONCLUSION: Polyamidoamine had cytotoxicity to 293T cells. The cell viability at 4 μmol/L concentration after 48 hours incubation was only 52%, and the acetylation could significantly decrease the cytotoxicity of polyamidoamine (P < 0.01). 293T cells incubated with polyamidoamine shrank and had bad stretching, while 293T cells incubated with acetylated polyamidoamine had good stretching. Acetylated polyamidoamine had no significant effect on the cell cycle, but polyamidoamine at 20 mg/L could block the cell cycle at S stage. All the results show that acetylation can decrease the cytotoxicity of polyamidoamine.

Key words: biomaterials, material biocompatibility, polyamidoamine, acetylation, cytotoxicity, 293T cells, proliferation, cell cycle, the National Natural Science Foundation of China, biomaterial photographs-containing paper

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