中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (47): 8775-8778.doi: 10.3969/j.issn.2095-4344.2012.47.008

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

牙釉质提取物钙磷胶体的合成材料与四环素改性

邓 炜1，魏春萍2，刘新征1   

  1. 赣南医学院第一附属医院，1口腔科，2耳鼻咽喉科，江西省赣州市 341000
  • 收稿日期:2012-04-09 修回日期:2012-08-05 出版日期:2012-11-18 发布日期:2012-11-18
  • 作者简介:邓炜★，男，1969年生，江西省宁都县人，2001年武汉大学口腔医学院毕业，硕士，副主任医师，副教授，主要从事口腔修复研究。 dwdent2@163.com

Tetracycline-modified calcium-phosphorus colloid: The synthetic material extracted from dental enamel

Deng Wei1, Wei Chun-ping2, Liu Xin-zheng1   

  1. 1Department of Stomatology, 2Department of Otolaryngology, First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China
  • Received:2012-04-09 Revised:2012-08-05 Online:2012-11-18 Published:2012-11-18
  • About author:Deng Wei★, Master, Associate chief physician, Associate professor, Department of Stomatology, First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China dwdent2@163.com

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被引次数

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摘要：

背景：研究表明人工合成羟基磷灰石的生物性能与天然牙、骨组织仍存在一定差异。
目的：分析牙釉质提取物合成材料的成分与晶体特性。
方法：将人牙釉质粉末经酸碱处理后分组：牙釉质粉末组、牙釉质粉末+四环素组、牙釉质粉末+四环素+几丁质组、牙釉质粉末+四环素+几丁质+液相组。后3组对磷酸化牙釉质行四环素改性，再分别按照顺序加入其他药物。液相组成为将0.125 mol/L NaH2PO4、0.125 mol/L Na2HPO4 溶解于蒸馏水。
结果与结论：①场发射扫描电镜观察：牙釉质的基本结构单元为纳米纤维，纳米纤维由很多细小的晶体组成。②X射线衍射分析：牙釉质经四环素改性后，磷灰石的基本物相组成由单一成分变成多种物相共存，结晶度降低，加入几丁质后结晶度明显提高。③红外光谱分析：各组样品中均含有PO43-，OH-吸收峰，牙釉质粉末+四环素组存在CO32-峰。说明牙釉质提取物合成后的主要成分是羟基磷灰石，经四环素改性后磷灰石的物相、结晶度、微量成分等结晶学特性发生了较明显改变，添加几丁质可提高四环素改性后磷灰石的结晶度。

关键词: 牙釉质粉末, 牙釉质提取物, 羟基磷灰石, 晶体, 四环素改性, 几丁质, 生物材料

Abstract:

BACKGROUND: Although the artificial hydroxyapatite is similar as the structure of inorganic composition of human sclerous tissues, its biological property has determinate difference compared to natural tooth and bone tissue.
OBJECTIVE: To analyze the components and crystal characteristics of the synthetic material extracted from dental enamel.
METHODS: The dental enamel power of clinical orthodontic teeth was collected. The power was treated with acid base, modified by tetracycline and then to form the tetracycline-calcium-phosphorus colloid. There were four groups including Group 1 (the dental enamel power), Group 2 (the dental enamel power+tetracycline), Group 3 (the dental enamel power+tetracycline+chitin) and Group 4 (the dental enamel power+tetracycline+chitin+liquid phase). All of them were detected by X-ray diffraction, infrared spectrum and field emission scan electron microscope. Liquid phase was prepared by 0.125 mol/L NaH2PO4 and 0.125 mol/L Na2HPO4 dissolved in distilled water.
RESULTS AND CONCLUSION: The basic phase composition of apatite changed from the single component to multiple phases after the dental enamel was modified by tetracycline. The crystallinity decreased after the dental enamel extraction was modified by tetracycline; however, the adamantine crystallinity obviously increased after the chitin was added. These findings suggest that the main component of the dental enamel extraction is hydroxyapatite; the crystallographic characteristics of apatite, such as the phase, crystallinity and microconstituenis content has obvious modification after the dental enamel is modified by tetracycline; the crystallinity of apatite modified by tetracycline could increase by adding the chitin.

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