中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8543-8550.doi: 10.3969/j.issn.2095-4344.2012.45.034

• 干细胞学术探讨 stem cell academic discussion • 上一篇    

干细胞移植用于肝再生治疗的研究

杨 屹，陈 熹，徐 心，吴 涛，黎一鸣   

  1. 西安交通大学医学院第二附属医院普外科，陕西省西安市 710004
  • 收稿日期:2012-08-05 修回日期:2012-09-12 出版日期:2012-11-04 发布日期:2012-11-04
  • 通讯作者: 黎一鸣，硕士，教授，主任医师，博士后导师，西安交通大学医学院第二附属医院普外科，陕西省西安市 710004 Liyiming2003@sina.com
  • 作者简介:杨屹☆，男，1972年生，陕西省户县人，汉族，西安交通大学医学院博士后在读，主治医师，主要从事肝脏再生及梗阻性黄疸研究。 yangyi2009@mail.xjtu.edu.cn

Stem cells transplantation for liver regeneration

Yang Yi, Chen Xi, Xu Xin, Wu Tao, Li Yi-ming   

  1. Department of General Surgery, Second Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi’an 710004, Shaanxi Province, China
  • Received:2012-08-05 Revised:2012-09-12 Online:2012-11-04 Published:2012-11-04
  • Contact: Li Yi-ming, Master, Professor, Chief physician, Postdoctoral supervisor, Department of General Surgery, Second Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi’an 710004, Shaanxi Province, China Liyiming2003@sina.com
  • About author:Yang Yi☆, Doctor, Attending physician, Department of General Surgery, Second Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi’an 710004, Shaanxi Province, China yangyi2009@mail.xjtu.edu.cn

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418

被引次数

3

摘要：

背景：肝脏终末期疾病的常规综合治疗不能达到理想的治疗效果，因此，干细胞移植治疗肝脏疾病成为研究的热点之一，并且已经成为治疗肝脏疾病的新方法。
目的：明确干细胞移植用于肝再生治疗的实验研究和临床应用结果。
方法：分别对不同来源的干细胞如骨髓干细胞、胚胎干细胞、脂肪干细胞以及外周血造血干细胞移植分化治疗肝脏疾病进行体外和体内实验研究分析，并且应用免疫组化染色、病理学以及血清中各项生化指标检测与正常肝脏细胞的生物特性及功能进行比较分析，确定干细胞移植治疗肝脏疾病的效果。
结果与结论：骨髓干细胞、胚胎干细胞、脂肪干细胞以及外周血造血干细胞均可以在特定条件下诱导分化成为肝样细胞，对损伤肝脏不仅有治疗作用而且能够促进肝脏的修复和再生，同时还可以进行干细胞移植转基因治疗。除此之外，干细胞移植可以避免免疫排斥反应的发生，可用于治疗各种类型的肝脏疾病。

关键词: 肝脏, 干细胞, 移植, 肝再生, 门静脉栓塞, 粒细胞集落刺激因子\甲胎蛋白\白蛋白\肝损伤\肝细胞

Abstract:

BACKGROUND: Satisfactory therapeutic effects can not been acquired in treatment of end-stage liver diseases by the routine comprehensive treatments. Therefore, stem cells transplantation for treatment of liver disease has become an interesting issue and has been considered as a new method in treatment of this disease.
OBJECTIVE: To clarify the experimental research and clinical application of stem cells transplantation for liver regeneration.
METHODS: In vitro and in vivo experimental studies regarding liver disease treatment by transplantation of different kinds of stem cells were analyzed, including bone marrow stem cells, embryonic stem cells, adipose stem cells and peripheral blood stem cells. The biological characteristics and functions were compared between normal liver cells and transplanted cells using immunohistological staining, pathological observation and biochemical index detection to determine the treatment effects of stem cells transplantation for the treatment of liver disease.
RESULTS AND CONCLUSION: Bone marrow stem cells, embryonic stem cells, adipose stem cells and peripheral blood stem cells can be induced to differentiate into hepatocyte-like cells under certain conditions, and these cells have therapeutic effects on the injured liver and can also promote the repair and regeneration of the liver. Meanwhile, these cells can be used for transgenic therapy in stem cells transplantation. In addition, stem cells transplantation can be used for treatment of various liver diseases without immunological rejections.

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