中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8530-8534.doi: 10.3969/j.issn.2095-4344.2012.45.032

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

不同来源肝干细胞的定向分化潜能

魏春山1，唐海鸿1，童光东1，陈孝银2   

  1. 1广州中医药大学深圳附属医院，深圳市中医院，广东省深圳市 518033
    2暨南大学医学院，广东省广州市 510632
  • 收稿日期:2012-01-29 修回日期:2012-03-20 出版日期:2012-11-04 发布日期:2012-11-04
  • 通讯作者: 童光东，博士，主任医师，教授，博士生导师，广州中医药大学深圳附属医院，深圳市中医院，广东省深圳市 518033 tgd755@163.com
  • 作者简介:魏春山☆，男，1975年生，湖北省长阳县人，土家族，广州中医药大学博士，主治医师，从事肝胆疾病中西医结合临床与科研工作。 weichunshan@163.com

Directed differentiation of hepatic stem cells from different origins

Wei Chun-shan1, Tang Hai-hong1, Tong Guang-dong1, Chen Xiao-yin2   

  1. 1Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen 518033, Guangdong Province, China
    2Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China
  • Received:2012-01-29 Revised:2012-03-20 Online:2012-11-04 Published:2012-11-04
  • Contact: Tong Guang-dong, M.D., Chief physician, Professor, Doctoral supervisor, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen 518033, Guangdong Province, China tgd755@163.com
  • About author:Wei Chun-shan☆, M.D., Attending physician, Shenzhen Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen 518033, Guangdong Province, China weichunshan@163.com

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563

被引次数

2

摘要：

背景：目前，对不同来源肝干细胞的分化潜能尚未形成一致意见，在运用中也无统一标准和规范。
目的：综述不同来源肝干细胞定向分化的潜能。
方法：应用计算机检索1990年1月至2011年11月PubMed数据库相关文章，检索词为“differentiation，hepatic stem cell，liver stem cell”，并限定文章语言种类为English。同时计算机检索1990年1月至2011年11月中国知网数据库相关文章，检索词为“肝干细胞，分化”，并限定文章语言种类为中文。最终纳入符合标准的文献42篇。
结果与结论：绝大多数肝干细胞的基础研究集中在胚胎来源肝干细胞或卵圆细胞，而在临床研究中，脐血、骨髓等来源肝干细胞获得了更多的关注，业内对各来源肝干细胞的分化潜能尚未形成一致意见。文章综述不同来源肝干细胞分化潜能的差异，探讨不同诱导因子在其中的决定作用，从而为进一步筛选稳定可靠的诱导因子，改善诱导定向分化的稳定性打下基础。同时期待更多符合临床需求的基础研究支持肝干细胞的应用，在肝干细胞来源和诱导剂上逐步形成一致的共识，建立统一的干细胞采集标准，甚至临床操作规范。

关键词: 肝干细胞, 来源, 定向分化, 诱导因子, 微环境, 干细胞, 综述文献

Abstract:

BACKGROUND: The hepatic stem cells originated from embryo or hepatic oval cells were always studied actively in basic researches. But the other stem cells from umbilical cord blood or bone marrow are paid more attention in clinical study. There is no agreement on differentiation of varied hepatic stem cells.
OBJECTIVE: To summarize the potentiality of directed differentiation of hepatic stem cells from different origins.
METHODS: PubMed databases and China National Knowledge Infrastructure (CNKI) were retrieved for articles published from January 1990 to October 2011 in English and Chinese respectively with the searching words of differentiation, hepatic stem cell or liver stem cell. Articles in the same research field published in the authoritative journals or recently published (past 3 years) were included. Forty-two articles were included in the final analysis.
RESULTS AND CONCLUSION: The majority of basic studies of hepatic stem cells focus on embryo-derived hepatic stem cells or hepatic oval cells; while in the clinical studies, umbilical cord- and bone marrow-derived hepatic stem cells have been paid more attention. There is no agreement on differentiation potential of hepatic stem cells from various origins. This paper summarizes the difference of differentiation potential of hepatic stem cells from different origins and investigates the decisive effect of different inductors so as to provide strong evidence for further screening reliable inductors and improving directed induced differentiation. More basic studies involving hepatic stem cells are expected to meet clinical requirements. This would help to achieve agreement in origins and inductors of hepatic stem cells and establish uniform standards to collect stem cells or uniform clinical operation guideline.

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