中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7940-7944.doi: 10.3969/j.issn.2095-4344.2012.42.029

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

枸杞多糖干预糖尿病大鼠颌下腺组织中核因子κB的表达

朱建华1，李佳宜1，李维善1，刘继光2，肖 震1   

  1. 1佳木斯大学口腔医学院，黑龙江省佳木斯市 154004
    2佳木斯大学，黑龙江省佳木斯市 154007
  • 收稿日期:2012-01-17 修回日期:2012-02-27 出版日期:2012-10-14 发布日期:2012-10-14
  • 通讯作者: 刘继光，博士，教授，佳木斯大学，黑龙江省佳木斯市 154007
  • 作者简介:朱建华，女，1959年生，黑龙江省佳木斯市人，汉族，1982年佳木斯医学院毕业，教授，主要从事牙周病、黏膜癌前病变、白斑、扁平苔藓、白塞病、口干、灼口综合症等相关方面的研究。 Hailiuxing6@163.com

Lycium barbarum polysaccharides intervene nuclear factor kappa B expression in the submandibular gland tissue of diabetic rats

Zhu Jian-hua1, Li Jia-yi1, Li Wei-shan1, Liu Ji-guang2, Xiao Zhen1   

  1. 1School of Stomatology, Jiamusi University, Jiamusi 154004, Heilongjiang Province, China
    2Jiamusi University, Jiamusi 154007, Heilongjiang Province, China
  • Received:2012-01-17 Revised:2012-02-27 Online:2012-10-14 Published:2012-10-14
  • Contact: Liu Ji-guang, Doctor, Professor, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China
  • About author:Zhu Jian-hua, Professor, School of Stomatology, Jiamusi University, Jiamusi 154004, Heilongjiang Province, China Hailiuxing6@163.com

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摘要：

背景：核因子κB是一种广泛存在于体内多种细胞的核转录因子，能介导多种炎性递质转录表达，也参与细胞凋亡的调控。
目的：观察枸杞多糖对糖尿病大鼠颌下腺组织中核因子κB表达的影响。
方法：60只Wistar大鼠随机分为正常对照组、糖尿病组、补枸杞多糖组，后2组用四氧嘧啶诱发糖尿病大鼠模型。造模成功后补枸杞多糖组灌胃50%枸杞多糖水溶液，糖尿病组和对照组灌胃等容量生理盐水，连续灌胃8周。
结果与结论：灌胃8周后糖尿病组和补枸杞多糖组体质量低于正常对照组(P < 0.05)，补枸杞多糖组体质量高于糖尿病组(P < 0.05)。灌胃8周后糖尿病组、补枸杞多糖组血糖高于正常对照组(P < 0.05)，补枸杞多糖组血糖低于糖尿病组(P < 0.05)。核因子κB在正常颌下腺中无表达或仅有少量表达；糖尿病时表达量明显增多，而补枸杞多糖组表达量则明显减少。结果可见枸杞多糖能够抑制核因子κB的活化与表达，对糖尿病大鼠颌下腺组织起到一定的保护作用。

关键词: 枸杞多糖, 核因子κB, 颌下腺, 糖尿病, 四氧嘧啶

Abstract:

BACKGROUND: Nuclear factor-κB is a nuclear transcription factor in a variety of cells, which can mediate transcriptional expression of various inflammatory transmitters and participate in the regulation of apoptosis.
OBJECTIVE: To observe the influence of Lycium barbarum polysaccharides (LBP) on the expression of nuclear factor-κB in the submandibular gland tissue of diabetic rats.
METHODS: Sixty Wistar rats were randomly divided into control, diabetes, LBP groups. Diabetic rat models were induced by alloxan in the latter two groups. After modeling, 50% LBP solution was intragastrically injected into the LBP group, and normal saline with the same volume was injected into the control and diabetes groups. The administration lasted for 8 weeks.
RESULTS AND CONCLUSION: After 8 weeks, the body mass of rats in the diabetes and LBP group was less than that in the control group (P < 0.05), and the rats in the LBP had a higher body mass as compared with those in the diabetes group (P < 0.05). The blood glucose in the diabetes and LBP group was higher than that in the control group (P < 0.05), while the blood glucose was lower in the LBP group compared with the diabetes goriup (P < 0.05). Expression of nuclear factor-κB was not found or little in the normal submandibular gland, but increased in the submandibular gland of diabetic rats and then decreased significantly after LBP treatment. These findings indicate that LBP can inhibit nuclear factor-κB activation and expression, and play a protective role in the submandibular gland tissue of diabetic rats.

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