中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7933-7939.doi: 10.3969/j.issn.2095-4344.2012.42.028

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

深Ⅱ度烫伤创面早期应用重组人表皮生长因子后表皮生长因子受体的表达

黄新稿，梁自乾，丁华荣   

  1. 广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市 530021
  • 收稿日期:2012-03-01 修回日期:2012-04-11 出版日期:2012-10-14 发布日期:2013-04-08
  • 通讯作者: 梁自乾，硕士，教授，硕士生导师，广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市 530021 liangzqian@yahoo.com
  • 作者简介:黄新稿★，男，1984年生，广西壮族自治区武宣县人，壮族，2012年广西医科大学毕业，硕士，主要从事烧伤创面修复重建方面的研究。 408409317@qq.com

Effect of recombinant human epidermal growth factor used at the early phase of wound healing on expression of epidermal growth factor receptor in deep Ⅱ scald wound

Huang Xin-gao, Liang Zi-qian, Ding Hua-rong   

  1. Department of Burns and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Received:2012-03-01 Revised:2012-04-11 Online:2012-10-14 Published:2013-04-08
  • Contact: Liang Zi-qian, Master, Professor, Master’s supervisor, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, China Liangzqian@yahoo.com
  • About author:Huang Xin-gao★, Master, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China 408409317@qq.com

PDF

612

被引次数

8

摘要：

背景：有研究表明重组人表皮生长因子能促进烧伤创面愈合，内源性表皮生长因子通过与表皮生长因子受体结合发挥生物学效应。
目的：观察局部应用重组人表皮生长因子对大鼠烫伤创面表皮生长因子受体的影响，分析其促进创面愈合的可能机制。
方法：制备Wistar大鼠背部深Ⅱ度烫伤模型，分为2组，对照组于创面喷洒生理盐水，实验组喷洒重组人表皮生长因子。烫伤后0，1，3，5，7，10，14，21 d取创面组织，采用Western blot检测表皮生长因子受体蛋白表达，并测定两组大鼠创面愈合率。
结果与结论：烫伤后7-21 d 实验组创面愈合率高于对照组(P < 0.05)。两组表皮生长因子受体蛋白表达在1 d明显降低(P < 0.05)，随后又升高，7 d达峰值且对照组比实验组高(P < 0.05)；峰值过后，对照组逐渐下降，21 d接近烫伤前水平，实验组迅速下降，14 d达谷值，随后上升，21 d接近烫伤前水平，两组差异有显著性意义(P < 0.05)。说明早期局部应用重组人表皮生长因子可影响表皮生长因子受体的表达规律，并显著促进大鼠深Ⅱ度烫伤创面愈合。

关键词: 深Ⅱ度烫伤, 创面愈合, 重组人表皮生长因子, 表皮生长因子受体, 大鼠

Abstract:

BACKGROUND: Previous studies have demonstrated that recombinant human epidermal growth factors can obviously accelerate wound closure on burning wound, and endogenous epidermal growth factor induces biological effects in combination with epidermal growth factor receptor.
OBJECTIVE: To investigate the effect of local recombinant human epidermal growth factors on the expression of epidermal growth factor receptor in the healing of scald wound in rats.
METHODS: Wistar rats were prepared for back deep Ⅱ degree scald models and divided into two groups. Rats in the control group were sprayed physiological saline and those in the experimental group were sprayed recombinant human epidermal growth factors. The healing rate of the wound was observed and expression of epidermal growth factor receptor protein was determined by Western-blot at 0, 1, 3, 5, 7, 10, 14, 21 days after operation.
RESULTS AND CONCLUSION: The wound healing rates of the experimental group were higher than those of the control group from 7 to 21 days (P < 0.05). The expression of epidermal growth factor receptor in the two groups were reduced at 1 day, and raised to the peak level at 7 days, which was higher in the control group than the experimental group (P < 0.05). After the peak level, the expression of epidermal growth factor receptor in the control group was reduced slowly to the normal level. While, the expression of epidermal growth factor receptor in the experimental group was reduced rapidly to a minimum at 14 days; after that, it was raised slowly and closed to the normal level. The two groups showed significant differences (P < 0.05). These findings indicate that local application of recombinant human epidermal growth factors at the early phase of wound healing can produce an influence on the expression of epidermal growth factor receptor and obviously accelerate wound closure in deep Ⅱ scald wound rats.

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