中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7929-7932.doi: 10.3969/j.issn.2095-4344.2012.42.027

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

缺氧诱导因子1在创伤性深静脉兔模型血栓形成及消退过程中的表达

刘海平，赵学凌，吴雪梅，周如丹，李宏昆，李兴国，郑宏宇   

  1. 昆明医学院第一附属医院骨科，云南省骨科研究中心，云南省昆明市 650032
  • 收稿日期:2012-06-04 修回日期:2012-08-20 出版日期:2012-10-14 发布日期:2012-10-14
  • 通讯作者: 赵学凌，主任医师，教授，昆明医学院第一附属医院骨科，云南省骨科研究中心，云南省昆明市 650032
  • 作者简介:刘海平，男，山西省朔州市人，昆明医学院第一附属医院骨科在读博士。

Hypoxia-inducible factor-1 expression in thrombosis and thrombolysis of a rabbit model of traumatic deep vein thrombosis

Liu Hai-ping, Zhao Xue-ling, Wu Xue-mei, Zhou Ru-dan, Li Hong-kun, Li Xing-guo, Zheng Hong-yu   

  1. Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Orthopedics Research Center of Yunnan Province, Kunming 650032, Yunnan Province, China
  • Received:2012-06-04 Revised:2012-08-20 Online:2012-10-14 Published:2012-10-14
  • Contact: Zhao Xue-ling, Chief physician, Professor Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Orthopedics Research Center of Yunnan Province, Kunming 650032, Yunnan Province, China wxxm@ynmail.com
  • About author:Liu Hai-ping☆, Studying for doctorate, Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Orthopedics Research Center of Yunnan Province, Kunming 650032, Yunnan Province, China l.xp1982@163.com

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摘要：

背景：深静脉血栓形成及消退过程中的分子机制极其复杂，目前已有研究表明，缺氧与损伤因素参与了深静脉血栓形成及消退过程。
目的：观察静脉壁中缺氧诱导因子1在创伤性深静脉血栓模型兔血栓形成及消退过程中的表达变化。
方法：将日本大耳白兔采用钳夹双侧股静脉+石膏固定双下肢建立兔创伤性深静脉血栓模型。PCR及ELISA检测股静脉组织中缺氧诱导因子1 mRNA、蛋白在兔创伤性深静脉血栓建模后表达的变化。
结果与结论：模型兔创伤后24 h，经B超监测，血栓形成率为58%；血栓栓子随造模后时间延长逐渐缩小机化，创伤后第5天开始经B超监测模型组血栓形成的血管腔有断续血流信号。模型兔股静脉组织中缺氧诱导因子1在造模后1，3，5 d表达逐渐升高(P < 0.05或P < 0.01)，此后7-14 d表达逐渐下降(P < 0.05或P < 0.01)。结果证实，在创伤性深静脉血栓形成过程，缺氧诱导因子1表达上调，在血栓形成后的溶解及机化再通过程中，缺氧诱导因子1表达下调。

关键词: 创伤, 深静脉血栓, 缺氧诱导因子1, 组织构建

Abstract:

BACKGROUND: The molecular mechanism of deep vein thrombosis and further regression is extremely complex. Researches show that hypoxia and injury factors are involved in deep vein thrombosis and regression.
OBJECTIVE: To investigate changes in hypoxia inducible factor (HIF)-1 expression during deep vein thrombosis and regression in a rabbit model of traumatic deep vein thrombosis (TDVT).
METHODS: The bilateral femoral vein of rabbits was clamped and both lower extremities were fixed with gypsum to establish model of TDVT. SYBR®Green I fluorescence real-time quantitative PCR and ELISA dynamic detection were used to determine the change of HIF-1 mRNA and protein expression in femoral vein in the rabbits with TDVT.
RESULTS AND CONCLUSION: At 24 hours post-injury, B-ultrasonography showed that thrombosis rate was 58% and thromboembolus gradually reduced and be organized with time; from the 5th day intermittent flow signals were monitored in lumen of blood vessel with thrombosis of model group. HIF-1 expression in vein of model group gradually increased (P < 0.05 or P < 0.01), and reduced between 7-14 days (P < 0.05 or P < 0.01). Those findings indicate that HIF-1 expression is upregulated during TDVT, but downregulated during thrombolysis and organization.

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