中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7925-7928.doi: 10.3969/j.issn.2095-4344.2012.42.026

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

血小板衍化生长因子BB对体外培养肌腱细胞增殖的影响

谢爱国1，梁红伟2   

  1. 1南京医科大学第二附属医院烧伤整形外科，江苏省南京市 210011
    2郑州市人民医院整形外科，河南省郑州市 450003
  • 收稿日期:2012-01-26 修回日期:2012-02-22 出版日期:2012-10-14 发布日期:2012-10-14
  • 作者简介:谢爱国★，男，1982年生，江苏省南京市人，汉族，2008年南京医科大学毕业，硕士，医师，主要从事组织损伤修复的基础与临床研究。

Effect of platelet-derived growth factor-BB on proliferation of tendon cells cultured in vitro

Xie Ai-guo1, Liang Hong-wei2   

  1. 1Department of Burns and Plastic Surgery, Second Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China
    2Department of Burns and Plastic Surgery, Zhengzhou People’s Hospital, Zhengzhou 450003, Henan Province, China
  • Received:2012-01-26 Revised:2012-02-22 Online:2012-10-14 Published:2012-10-14
  • About author:Xie Ai-guo★, Master, Physician, Department of Burns and Plastic Surgery, Second Hospital of Nanjing Medical University, Nanjing 210011, Jiangsu Province, China

PDF

394

被引次数

5

摘要：

背景：细胞增殖和组织修复是一个复杂的过程，这期间有大量生长因子参与，并且这些因子在发挥本身功效时，还相互影响和制约，从而更好的促进细胞增殖和组织修复。
目的：了解不同质量浓度的血小板衍化生长因子BB对体外培养肌腱细胞增殖的影响。
方法：体外培养并鉴定大鼠肌腱细胞，根据培养液中血小板衍化生长因子BB质量浓度的不同分为对照组(0 μg/L)和实验组(1，5，10，20，50，100，150，200，250 μg/L)，以MTT法检测细胞增殖能力。
另外检测对照组与20 μg/L血小板衍化生长因子BB组培养12-72 h时肌腱细胞的吸光度值，以观察其增殖量效与时间的关系。
结果与结论：分离培养的细胞Ⅰ型胶原染色呈阳性， Ⅲ型胶原染色呈阴性，证明为大鼠肌腱细胞。与对照组相比，除 250 μg/L 血小板衍化生长因子BB组外，其他各实验组细胞的吸光度值均升高(P < 0.05或P < 0.01)；并且随血小板衍化生长因子BB质量浓度的增加，其吸光度值先逐渐增大，达一定浓度(20 μg/L)后，又逐渐减小。在20 μg/L血小板衍化生长因子BB组，肌腱细胞数量从培养12 h开始增加，至48 h达高峰，在培养24-72 h的吸光度值均显著高于对照组(P < 0.01)。结果可见血小板衍化生长因子BB在一定质量浓度和时间范围内具有促进肌腱细胞增殖的作用。

关键词: 血小板衍化生长因子BB, 肌腱细胞, 增殖, 细胞培养, 体外

Abstract:

BACKGROUND: Cell proliferation and tissue repair is a complicated process, accompanied by participation of multiple growth factors. These factors interact with each other to better promote cell proliferation and tissue repair.
OBJECTIVE: To investigate the effect of platelet-derived growth factor-BB (PDGF-BB) of different concentrations on proliferation of tendon cells cultured in vitro.
METHODS: Rat tendon cells were cultured and identified in vitro. According to concentration of PDGF-BB nutrient solution in culture medium, the cells were divided into control group (0 μg/L) and PDGF-BB groups (1, 5, 10, 20, 50, 100, 150, 200, 250 μg/L). Proliferation of tendon cells was detected by MTT test. The absorbance value of tendon cells in control group and 20 μg/L PDGF-BB group after culture for 12, 24, 36, 48, 60 and 72 hours were determined to evaluate relationship with proliferation dose-effect and time.
RESULTS AND CONCLUSION: The isolated cells were identified to be tendon cells as they were positive for type Ⅰ collagen staining and negative for type Ⅲ collagen staining. Compared with control group, the absorbance values of all groups were all increased, except for 250 μg/L PDGF-BB group (P < 0.05 or P < 0.01). Besides, the absorbance value rose gradually with the increase of the concentration of PDGF-BB, and then diminished gradually with the increase of the concentration of PDGF-BB from 20 μg/L. The number of tendon cells in 20 μg/L PDGF-BB group began to increase when cultured for 12 hours, and reached the highest level at 48 hours, and the absorbance at 24-72 hours was significantly greater than control group (P < 0.01). Results suggest that PDGF-BB can promote the proliferation of tendon cells in a definite range of concentration and time．

中图分类号: