中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7901-7908.doi: 10.3969/j.issn.2095-4344.2012.42.022

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

增龄犬左心房肌细胞动作电位和L型钙通道的变化

甘天翊1，许国军1，周贤惠1，汤宝鹏1，郭 霞2，李耀东1，姜 涛3，张 健1，李发鹏1   

  1. 新疆医科大学第一附属医院，1起搏电生理科，3医学研究中心，新疆维吾尔自治区乌鲁木齐市 830054；2新疆医科大学地方病重点实验室，新疆维吾尔自治区乌鲁木齐市 830000
  • 收稿日期:2012-08-01 修回日期:2012-08-27 出版日期:2012-10-14 发布日期:2012-10-14
  • 通讯作者: 汤宝鹏，博士，主任医师，教授，博士生导师，新疆医科大学第一附属医院起搏电生理科，新疆维吾尔自治区乌鲁木齐市 830054 tangbaopeng@hotmail.com
  • 作者简介:甘天翊☆，男，1980年生，新疆维吾尔自治区乌鲁木齐市人，汉族，博士，主治医师，主要从事心律失常的研究。 taog666@sina.com

Changes of action potential and L-type calcium channel of left atrial myocytes in the aging-associated rats

Gan Tian-yi1, Xu Guo-jun1, Zhou Xian-hui1, Tang Bao-peng1, Guo Xia2, Li Yao-dong1, Jiang Tao3, Zhang Jian1, Li Fa-peng1   

  1. 1Department of Cardiology, 3Medical Research Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 2Key Laboratory of Endemic Disease, Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • Received:2012-08-01 Revised:2012-08-27 Online:2012-10-14 Published:2012-10-14
  • Contact: Tang Bao-peng, Doctor, Chief physician, Professor, Doctoral supervisor, Department of Cardiology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China tangbaopeng@hotmail.com
  • About author:Gan Tian-yi, Doctor, Attending physician, Department of Cardiology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China taog666@sina.com.cn

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被引次数

1

摘要：

背景：增龄是心房颤动发生的独立危险因素，心房肌细胞电生理特性的变化是触发和维持心房颤动的重要因素。
目的：观察不同年龄犬左心房肌细胞动作电位和L型钙通道的改变。
方法：实验纳入7只成年犬和10只老年犬，用Ⅱ型胶原酶分离成年犬和老年犬的左心房肌细胞，用全细胞膜片钳方法记录成年犬和老年犬左心房肌细胞动作电位和L型钙通道电流；采用实时荧光定量PCR和Western blot检测左心房肌L型钙通道α1c亚基mRNA和蛋白的表达。
结果与结论：与成年犬相比，老年犬左心房肌细胞动作电位平台期的幅度明显降低(P < 0.05)，动作电位时程明显延长(P < 0.05)，而最大舒张电位和动作电位幅度在老年犬和成年犬间差异无显著性意义(P > 0.05)；同时老年犬左心房肌细胞的L型钙通道电流密度较成年犬明显降低(P < 0.05)，而L型钙通道电流动力学参数在成年犬和老年犬间差异无显著性意义(P > 0.05)；此外，与成年犬相比，老年犬左心房肌L型钙通道α1c亚基mRNA和蛋白的表达明显降低(P < 0.05)。由此认为，左心房肌细胞电生理学特性存在增龄性改变。

关键词: 心房颤动, 增龄, 左心房, 肌细胞, L型钙通道, 钙电流, 动作电位, 细胞电生理, L型钙通道α1c亚基, 成年, 老年

Abstract:

BACKGROUND: Aging is the independent risk factor of atrial fibrillation. The aging-associated changes of electrophysiological properties of atrial myocytes may play an important role in the mechanisms of atrial fibrillation.
OBJECTIVE: To investigate the aging-associated changes of action potential and L-type calcium channel of left atrial myocytes.
METHODS: Seventeen canines were allocated to two groups, including seven adult canines and 10 old canines. The left atrial myocytes were separated from the adult and old canines by collagenase-Ⅱ. We used whole-cell patch-clamp to record action potential and L-type calcium current in left atrial myocytes of adult and older canines. The α1c subunit mRNA and protein expression of L-type calcium channel were assessed by real-time quantitative PCR and Western blotting respectively.
RESULTS AND CONCLUSION: Compared with the adult canines, the amplitude of left atrial myocytes action potential plateau in old canines was significantly decreased (P < 0.05), and the action potential duration was significantly prolonged (P < 0.05), while there was no significant difference of the maximum diastolic potential and action potential amplitude (P > 0.05); the L-type calcium channel current density of the left atrial myocytes in the old canines was lower than that in the adult canines (P < 0.05), while there was no significant difference of the current kinetic parameters of L-type calcium channel between old and adult canines (P > 0.05); compared with the adult canines, the α1c subunit mRNA and protein expression of L-type calcium channel in the old canines was significantly decreased (P < 0.05). There are different aging-associated cell electrophysiological changes in the left atrial myocytes.

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