中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7897-7900.doi: 10.3969/j.issn.2095-4344.2012.42.021

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

Smad2/3信号分子在小鼠胚胎腭发育和腭裂形成中的表达

王 莎1，宋 容1，王利婷1，张 瑞1，司庆宗1，王建林2，赵望泓1, 3   

  1. 1兰州大学口腔医学院，甘肃省兰州市 730000
    2兰州大学生命科学学院，甘肃省兰州市 730000
    3南方医科大学口腔医学院，广东
  • 收稿日期:2012-03-14 修回日期:2012-05-14 出版日期:2012-10-14 发布日期:2012-10-14
  • 通讯作者: 赵望泓，博士，教授，兰州大学口腔医学院，现工作单位为南方医科大学南方医院口腔医疗中心，广东省广州市 510515 zhaowh@lzu.edu.cn
  • 作者简介:王莎★，女，1984年生，山东省即墨市人，汉族，兰州大学口腔医学院在读硕士，主要从事口腔发育生物学的研究。 kqwangsha@163.com

Expression of Smad2/3 in normal palate development and cleft palate formation during mouse embryogenesis

Wang Sha1, Song Rong1, Wang Li-ting1, Zhang Rui1, Si Qing-zong1, Wang Jian-lin2, Zhao Wang-hong1, 3   

  1. 1School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China
    2School of Life Science, Lanzhou University, Lanzhou 730000, Gansu Province, China
    3College of Stomatology, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Received:2012-03-14 Revised:2012-05-14 Online:2012-10-14 Published:2012-10-14
  • Contact: Wang Jian-lin, Doctor, Professor, School of Life Science, Lanzhou University, Lanzhou 730000, Gansu Province, China jlwang@lzu.edu.cn
  • About author:Wang Sha★, Studying for master’s degree, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China kqwangsha@163.com

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556

被引次数

1

摘要：

背景：部分学者通过体外器官培养研究认为全反式维甲酸干扰了Smad2/3在腭部的表达，具体机制尚不明确。
目的：观察腭突Smad2/3信号分子在胚鼠腭部发育及腭裂形成过程中的表达变化。
方法：54只C57BL/6J近交系孕鼠随机分为3组，在妊娠10 d，实验组一次性灌胃全反式维甲酸100 mg/kg诱导胚鼠两侧腭突不能在中线融合，建立腭裂畸形动物模型；植物油对照组灌胃10 mL/kg橄榄油，空白对照组不做处理。
结果与结论：在植物油对照组中，从妊娠13 d 18时到妊娠14 d 18时腭间充质细胞中Smad2/3免疫阳性表达逐步增高，至妊娠15 d 8时表达开始出现下降，实验组也表现为这一变化趋势，且同一组间表达较植物油对照组明显；在腭中嵴上皮细胞中，植物油对照组随着腭突的融合，腭中嵴上皮带消失，Smad2/3表达也明显下降，实验组始终未融合，未见明显的Smad2/3阳性细胞。在整个胚腭正常发育和腭裂形成过程中，空白对照组与植物油对照组Smad2/3阳性细胞表达几乎无差异。提示过量全反式维甲酸可能通过干扰腭中嵴上皮细胞及间充质细胞中Smad2/3信号分子的表达，从而影响小鼠胚腭上皮间充质转化，与腭裂形成密切相关。

关键词: 全反式维甲酸, Smad2/3, 腭裂, 小鼠, 免疫组织化学

Abstract:

BACKGROUND: Through in vitro organ culture studies, a few scholars consider that all-trans retinoic acid (at RA) can interfere with palatal expression, but the underlying mechanisms remain unclear.
OBJECTIVE: To investigate the expression changes of Smad2/3 of palatal shelves in normal palatal development and cleft palate formation during mouse embryogenesis.
METHODS: Both sides of the embryonic mouse palatal shelves were induced by excess at RA so that they could not integrate at the midline. Then, a mouse model of cleft palate was established, and 54 C57BL/6J inbred pregnant mice were randomly divided into experimental group, vegetable oil control group and blank control group. At GD 10, the mice in the experimental group were subjected to once gastric perfusion with 100 mg/kg at RA, and those in the vegetable oil control group underwent lavage with 10mL/kg olive oil, while those in the blank control group had no treatment.
RESULTS AND CONCLUSION: Smad2/3 positive expression was increased gradually in the embryonic palatal mesenchymal cells from GD1318 to GD148, but few positive cells were observed after GD158, while this trend was also found in the experimental group. In addition, Smad2/3 positive expression at the same developmental time was more obvious than that of the vegetable oil control group. In palatal medial edge epithelium, with the integration of bilateral palatal shelves, the ridge in palate belt disappeared, and Smad2/3 expression was decreased significantly. In the experimental group，the integration did not appeared, besides, significant Smad2/3 positive cells were not found. There was no significant difference in Smad2/3 positive cell expression between the blank control and vegetable oil groups in normal palatal development and cleft palate formation during mouse embryogenesis. These results suggest that excess at RA can interfere with Smad2/3 expression in the embryonic palatal medial edge epithelium and embryonic palatal mesenchymal cells, and thereby influence epithelial-mesenchymal transformation in mice, which is closely related to the formation of cleft palate.

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