中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (37): 6876-6880.doi: 10.3969/j.issn.2095-4344.2012.37.008

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

移植性鳞癌兔模型的建立及生物学特征

邢在臣1，丁国伟2，艾德里3○   

  1. 1泰山医学院附属医院口腔科，山东省泰安 271000
    2 同济大学医院管理处，上海市 200092
    3Kamulegeya Adriane，金贾，乌干达 999123
  • 收稿日期:2012-06-04 修回日期:2012-07-18 出版日期:2012-09-09 发布日期:2012-09-09
  • 通讯作者: 丁国伟，同济大学医院管理处，上海市 200092 tjhosp698@sohu.com
  • 作者简介:邢在臣★，男，1971年生，汉族，山东省泰安市人，硕士，2006年同济大学毕业，主治医师，主要从事口腔颌面外科临床工作。 xingzch@163.com

Establishment and the biological characteristics of transplanted squamous cell carcinoma models in rabbits

Xing Zai-chen1, Ding Guo-wei2, Kamulegeya Adriane3   

  1. 1Department of Stomatology, Affiliated Hospital of Taishan Medical College, Taian 271000, Shandong Province, China
    2Division of Hospital Administration, Tongji University, Shanghai 200092, China
    3Kamulegeya Adriane, Jinja 999123, Uganda
  • Received:2012-06-04 Revised:2012-07-18 Online:2012-09-09 Published:2012-09-09
  • Contact: Ding Guo-wei, Division of Hospital Administration, Tongji University, Shanghai 200092, China tjhosp698@sohu.com
  • About author:Xing Zai-chen★, Master, Attending physician, Department of Stomatology, Affiliated Hospital of Taishan Medical College, Tai’an 271000, Shandong Province, China xingzch@163.com

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420

被引次数

4

摘要：

背景：兔VX2荷瘤目前已广泛应用于全身多个器官肿瘤动物模型的建立，但在耳郭建立鳞癌动物模型至今少有报道。
目的：采用2种不同移植瘤方法建立稳定的兔耳VX2移植瘤模型，并对所建肿瘤模型的生物学特性进行观察和比较。
方法：将新西兰大白兔分别用瘤细胞悬液注入法和瘤组织块切开植入法移植于兔耳皮下，观察和比较 2种方法所建立的模型的肿瘤生长状况、成瘤率和自发转移发生率。
结果与结论：瘤细胞悬液注入组和瘤组织块植入组的成瘤率分别为73.3%和50%，差异有显著性意义(P < 0.05)。局部淋巴结转移率分别为75%和86%。两种方法建立的兔VX2鳞癌移植瘤模型中，组织呈浸润性生长，瘤细胞核深染，分裂相多见，并呈癌巢排列的符合鳞状细胞癌特征。证实，瘤细胞悬液注入法和瘤组织块切开植入法建立的移植瘤模型均较接近人鳞癌自然生长过程。瘤细胞悬液注入法建立的动物模型，因成瘤率和转移率高，更适合于鳞癌研究。

关键词: VX2肿瘤, 鳞癌, 动物模型, 耳郭, 生物学特征, 组织构建

Abstract:

BACKGROUND: Rabbit VX2 carcinoma has been widely used in building tumor models of multiple body organs, but it is rarely reported to be used for establishing the squamous cell carcinoma model of auricle.
OBJECTIVE: To establish the VX2 auricular tumor model in rabbits by two tumor transplantation methods and to investigate its biological characteristics.
METHODS: The VX2 cell suspensions were injected in the ears of New-Zealand rabbits by tumor cells suspension injection method and tumor tissue incision implantation method. Then the growth status of the tumors, the tumor-take rates and the metastasis rates of the models were evaluated.
RESULTS AND CONCLUSION: The tumor take-rate of tumor cells suspension injection group and tumor tissue incision implantation group was 73.3% and 50%, respectively, and the difference was significant (P < 0.05), metastases rate of lymph node in two groups was 75% and 85.7% respectively. In the rabbit VX2 squamous cell carcinoma transplanted models established by the two methods, the organization showed infiltrative growth, the deeply stained tumor nuclei and division phase were common, and cancer nest arranged cells were in line with the characteristics of the squamous cell carcinoma. It confirmed that the squamous cell carcinoma transplanted model established by tumor cell suspension injection method and tumor tissue incision implantation method was closed to the natural growth process of human squamous cell carcinoma. The animal model established by tumor cell suspension injection method is more suitable for the study of the squamous cell carcinoma due to the high rate of tumor formation and transfer rate.

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