中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (36): 6669-6673.doi: 10.3969/j.issn.2095-4344.2012.36.004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

尿酸抑制人骨髓间充质干细胞的成脂分化

朱晓琳，徐丽丽，杨乃龙   

  1. 青岛大学医学院附属医院黄岛院区内分泌科，山东省青岛市 266550
  • 收稿日期:2011-12-02 修回日期:2012-02-03 出版日期:2012-09-02 发布日期:2012-09-02
  • 通讯作者: 杨乃龙，硕士，主任医师，内分泌科主任，青岛大学医学院附属医院黄岛院区内分泌科，山东省青岛市 266550 nailongy@163.com
  • 作者简介:朱晓琳★，女，1987年生，山东省青岛市人，汉族，青岛大学医学院在读硕士，主要从事老年医学(内分泌与代谢病方向)方面的研究。 susansoffen@126.com

Uric acid inhibits adipogenic differentiation of human bone marrow mesenchymal stem cells

Zhu Xiao-lin, Xu Li-li, Yang Nai-long   

  1. Department of Endocrinology, Huangdao Branch, Affiliated Hospital of Qingdao University Medical College, Qingdao 266550, Shandong Province, China
  • Received:2011-12-02 Revised:2012-02-03 Online:2012-09-02 Published:2012-09-02
  • Contact: Yang Nai-long, Master, Chief physician, Department of Endocrinology, Huangdao Branch, Affiliated Hospital of Qingdao University Medical College, Qingdao 266550, Shandong Province, China nailongy@163.com
  • About author:Zhu Xiao-lin★, Studying for master’s degree, Department of Endocrinology, Huangdao Branch, Affiliated Hospital of Qingdao University Medical College, Qingdao 266550, Shandong Province, China susansoffen@126.com

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460

被引次数

11

摘要：

背景：研究表明一些药物可影响骨髓间充质干细胞的成骨、成脂诱导分化。
目的：观察尿酸对人骨髓间充质干细胞成脂分化的影响。
方法：全骨髓培养法体外分离培养人骨髓间充质干细胞，在成脂诱导条件下，分别加入0(对照组)，0.1，0.2，0.4，0.8 mmol/L浓度尿酸，诱导14，21 d时行油红O染色，倒置显微镜下计数成脂细胞数量。
结果与结论：成脂诱导14 d后，含不同浓度尿酸的诱导组成脂细胞数量较对照组明显减少(P < 0.05)，且随着尿酸浓度的增加，抑制成脂细胞生成的效果更为明显(P < 0.05)；成脂诱导21 d后，尿酸对骨髓间充质干细胞成脂诱导分化的抑制作用较诱导14 d时更加明显(P < 0.05)，同样随着尿酸浓度的增加，抑制作用更加明显(P < 0.05)。说明在体外尿酸能够抑制人骨髓间充质干细胞的成脂分化，并且存在一定的浓度依赖性和时间依赖性。

关键词: 骨髓间充质干细胞, 尿酸, 成脂分化, 高尿酸血症, 骨质疏松症, 干细胞

Abstract:

BACKGROUND: Studies have demonstrated that some drugs can influence the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To investigate the effect of uric acid on adipogenic differentiation of human BMSCs (hBMSCs).
METHODS: BMSCs were isolated and cultured using the whole bone marrow culture method. Under the adipogenic differentiation condition, BMSCs were exposed to five experimental concentrations of uric acid (0 mmol/L(control group), 0.1 mmol/L, 0.2 mmol/L, 0.4 mmol/L, 0.8mmol/L) for 14 days and 21 days. BMSCs were quantitated under phase-contrast inverted microscope after oil red O staining.
RESULTS AND CONCLUSION: After 14-days treatment with uric acid in adipogenic medium, BMSCs with positive oil red O staining significantly decreased compared to the control group (P < 0.05) and the inhibitory effect was more apparent as the increase in concentration of uric acid (P < 0.05). The inhibitory effect of uric acid on adipogenic differentiation of BMSCs was more significantly at 21 days than at 14 days (P < 0.05). The inhibitory effect also increased more significantly with increasing concentration of uric acid (P < 0.05). The result reveals that uric acid inhibits the adipogenic differentiation of BMSCs in a dose-dependent and time-dependent manner.

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