中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 5983-5987.doi: 10.3969/j.issn.2095-4344.2012.32.018

• 肿瘤干细胞 cancer stem cells • 上一篇    下一篇

人滑膜肉瘤细胞株SW982中肿瘤干细胞的分离及鉴定

黄 哲，锡林宝勒日   

  1. Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • 收稿日期:2012-03-13 修回日期:2012-05-23 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 锡林宝勒日，博士，主任医师，副教授，新疆医科大学附属肿瘤医院，新疆维吾尔自治区乌鲁木齐市 830011 xjbonetumor@126.com
  • 作者简介:黄哲★，男，1984年出生，山东省滕州市人，汉族，新疆医科大学在读硕士，主要从事为软组织肿瘤方面的研究。 huangzhe123168@163.com

Separation and identification of cancer stem cells in synovial sarcoma SW982 cell lines

Huang Zhe, Xilinbaoleri   

  1. 新疆医科大学附属肿瘤医院，新疆维吾尔自治区乌鲁木齐市 830011
  • Received:2012-03-13 Revised:2012-05-23 Online:2012-08-05 Published:2012-08-05
  • Contact: Xilinbaoleri, M.D., Chief physician, Associate professor, Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China xjbonetumor@126.com
  • About author:Huang Zhe★, Studying for master’s degree, Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China huangzhe123168@163.com

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被引次数

1

摘要：

背景：滑膜肉瘤属于高度恶性肿瘤，目前尚无有效治疗手段。肿瘤干细胞理论的提出为滑膜肉瘤的治疗提供了新的思路。
目的：探寻人滑膜肉瘤细胞系SW982中是否存在干细胞样细胞。
方法：首先采用不断剥夺血清浓度的方法培养SW982细胞，选择在最低血清浓度培养下的细胞进行抗药实验。MTT法测定在1，10，20，30，40，50，60 mg/L阿霉素浓度下的肿瘤细胞抑制率。裸鼠接种耐药细胞与非耐药细胞观察是否有肿瘤生成。
结果与结论：经过降血清培养后的SW982细胞的耐药性有所增强；在裸鼠成瘤实验中耐药细胞接种后有2只裸鼠成瘤，余均未成瘤。说明：①体外培养的人滑膜肉瘤细胞系SW982中存在具有高致瘤性的肿瘤干细胞样细胞。②采用高浓度阿霉素药物筛选细胞实验可以有效富集具有高致瘤性的滑膜肉瘤细胞群，进而富集滑膜肉瘤干细胞，但尚需进一步纯化。

关键词: 滑膜肉瘤, 肿瘤干细胞, 阿霉素, 裸鼠, MTT法, 干细胞

Abstract:

BACKGROUND: Synovial sarcomas are highly malignant tumor, but there is no effective treatment method. The tumor stem cell theory provides a new approach for the treatment of synovial sarcomas.
OBJECTIVE: To explore whether stem-like cells exist in synovial sarcoma SW982 cell lines.
METHODS: We cultured SW982 cells in a low concentration of serum medium, and the cells which were cultured in the lowest concentration of serum medium were selected for the drug-resistant experiment. The inhibition rate of tumor cells cultured under different concentrations of adriamycin (1, 10, 20, 30, 40, 50, 60 mg/L) were tested by MTT assay. We performed tumorigenicity experiments in the nude mice with the drug-resistant and non-drug-resistant cells respectively.
RESULTS AND CONCLUSION: Drug-resistant capacity of SW982 cells was improved after cultured with low concentration of serum. In the tumorigenicity experiments, there were two nude mice in which a tumor formed after injected with drug-resistant cells; and tumors did not form in the others. There are high tumorigenicity tumor stem-like cells in the SW982 cell lines. We can collect high tumorigenicity synovial sarcoma cell line effectively with high concentration of adriamycin, and enrich the synovial sarcoma stem cells, but the cells need further purification.

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