中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 5959-5962.doi: 10.3969/j.issn.2095-4344.2012.32.013

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

杜仲水/醇提取物促进兔脂肪基质干细胞增殖及成骨分化

郭延伟，李 松，房殿吉   

  1. 佳木斯大学附属口腔医院颌面外科，黑龙江省佳木斯市 154000
  • 收稿日期:2011-12-19 修回日期:2012-01-05 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 房殿吉，博士，副主任医师，佳木斯大学附属口腔医院颌面外科，黑龙江省佳木斯市 154000 dianjifang@yahoo.com.cn
  • 作者简介:郭延伟★，男，1985年生，山东省聊城市人，汉族，佳木斯大学在读硕士，主要从事组织工程中脂肪干细胞的增殖与分化研究。 guoweikq@126.com

Effects of eucommia water/alcohol extract on proliferation and osteogenic differentiation of rabbit adipose derived mesenchymal stem cells

Guo Yan-wei, Li Song, Fang Dian-ji   

  1. Department of Oral and Maxillofacial Surgery, Stomatology Hospital, Jiamusi University, Jiamusi 154000, Heilongjiang Province, China
  • Received:2011-12-19 Revised:2012-01-05 Online:2012-08-05 Published:2012-08-05
  • Contact: Fang Dian-ji, M.D., Associate professor, Department of Oral and Maxillofacial Surgery, Stomatology Hospital, Jiamusi University, Jiamusi 154000, Heilongjiang Province, China dianjifang@yahoo.com.cn
  • About author:Guo Yan-wei★, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Stomatology Hospital, Jiamusi University, Jiamusi 154000, Heilongjiang Province, China guoweikq@126.com

PDF

597

被引次数

12

摘要：

背景：传统中药杜仲可以促进脂肪间充质干细胞成骨分化。
目的：探讨杜仲水/醇提取物对脂肪基质干细胞的增殖作用和成骨分化影响。
方法：取兔脂肪组织体外培养脂肪基质干细胞，传代3次后进行细胞增殖及成骨诱导分化。分为诱导培养基中分别含有杜仲水/醇提取物 0.2，0.3，0.4，0.5 g/L 4个稀释度，对照组加入等量磷酸盐缓冲液。成骨诱导6 d后进行各组细胞增殖活力的检测和碱性磷酸酶的测定，14 d进行茜素红染色及钙化结节计数。
结果与结论：杜仲水/醇提取物对脂肪基质干细胞的增殖无明显影响，但均可明显刺激上调碱性磷酸酶的活性，相同稀释度下杜仲醇提取物的调节作用大于水提取物，且在0.4~0.5 g/L稀释度下对钙化结节的形成状况和数量有显著促进作用(P < 0.05)。说明杜仲水/醇提取物有促进脂肪基质干细胞向成骨细胞转化的作用，杜仲醇提取物作用优于杜仲水提取物。

关键词: 杜仲, 水提取物, 醇提取物, 脂肪基质干细胞, 成骨, 增殖

Abstract:

BACKGROUND: Conventional Chinese medicine eucommia can promote the osteogenic differentiation of adipose derived mesenchymal stem cells.
OBJECTIVE: To investigate the effects of eucommia water/alcohol extract on the proliferation and osteogenic differentiation of adipose derived mesenchymal stem cells.
METHODS: The rabbit adipose tissue was taken for in vitro culture of adipose derived mesenchymal stem cells. Passage 3 cells were used for study of cell proliferation ability and osteogenic differentiation. The experimental group cells were treated with induced medium containing 0.2, 0.3, 0.4, 0.5 g/L eucommia water/alcohol extract. The control group cells were treated with equal amounts of phosphate buffered saline. After osteogenic induction for 6 days, cell proliferation ability and alkaline phosphatase activity were determined. After osteogenic inducation for 14 days, alizarin red staining was performed and calcified nodules were counted.
RESULTS AND CONCLUSION: Eucommia water/alcohol extract did not produce effects on the proliferation of adipose derived mesenchymal stem cells, but it significantly up-regulated the activity of alkaline phsphatase. Eucommia alcohol extract exhibited stronger regulatory effects than eucommia water extract at the same dilutions, and eucommia water/alcohol extract at 0.4-0.5 g/L dilutions significantly promoted the formation of calcified nodules (P < 0.05). These findings suggest that eucommia water/alcohol extract promotes the osteogenic differentiation of adipose derived mesenchymal stem cells, and eucommia alcohol extract exhibits better effects than eucommia water extract.

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