中国组织工程研究

中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (4): 558-563.doi: 10.3969/j.issn.2095-4344.2369

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

多孔碳化硅涂覆生物活性钽新型生物医用材料的影响因素及生物学性能

马志杰1，2，李京育2，曹  放1，刘  蓉1，赵德伟2   

  1. 1大连理工大学电子信息与电气工程学部生物医学工程学院，辽宁省大连市   116024；2大连大学附属中山医院骨科，辽宁省大连市   116001
  • 收稿日期:2020-02-13 修回日期:2020-02-22 接受日期:2020-03-18 出版日期:2021-02-08 发布日期:2020-11-21
  • 通讯作者: 赵德伟，主任医师，教授，博士生导师，大连大学附属中山医院骨科，辽宁省大连市 116001
  • 作者简介:马志杰，男，1987年生，河南省周口市人，汉族，大连理工大学在读博士，主要从事骨科和医用多孔生物材料方向研究。 李京育，男，1994年生，辽宁省大连市人，汉族，研究实习员，主要从事医用多孔钽金属骨植入材料的研发与设计方面的研究。
  • 基金资助:
    大连市科技创新基金项目(2018J11CY030)；国家十三五“国家重点研发计划”项目(2016YFC1102004)

Influencing factors and biological property of novel biomedical materials: porous silicon carbide coated with bioactive tantalum

Ma Zhijie1,2, Li Jingyu2, Cao Fang1, Liu Rong1, Zhao Dewei2   

  1. 1School of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian 116024, Liaoning Province, China; 2Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • Received:2020-02-13 Revised:2020-02-22 Accepted:2020-03-18 Online:2021-02-08 Published:2020-11-21
  • Contact: Zhao Dewei, Chief physician, Professor, Doctoral supervisor, Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • About author:Ma Zhijie, Doctoral candidate, School of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian 116024, Liaoning Province, China; Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China Li Jingyu, Research assistant, Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • Supported by:
    the Dalian Science and Technology Innovation Fund Project, No. 2018J11CY030; the National Major Research and Development Plan during the Thirteenth Five-Year Plan Period, No. 2016YFC1102004

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摘要：

文题释义：
多孔碳化硅：通过较高温度的烧结工艺制备的多孔陶瓷材料，具备高开孔率、密度小、强度高、耐腐蚀等独特优点，其多孔结构具有与人体骨骼的小梁结构相似的生物形态结构，并且能够加工成各种复杂形状，具有良好的生物相容性。
钽金属：是一种坚硬、延展性、高度耐化学腐蚀的材料，与人体骨骼有很好的结合。自20世纪40年代以来，其已成功用于临床作为生物医用金属材料，但由于钽金属既昂贵又难以加工，钽作为生物材料的使用受到限制，通常以粉末形式生产，同时也可以作为涂层施加在固体和开放的多孔植入物表面上。

背景：多孔碳化硅具有出色的物理和化学特性，可用于修复复杂形状和较长的负重骨缺损，但其不具有生物活性，无法促进骨骼重塑与整合。
目的：在多孔碳化硅支架表面沉积钽涂层，评估其生物活性。
方法：运用化学气相沉积技术在多孔碳化硅支架表面沉积钽涂层，制作过程中通过设置不同的气体反应流量与温度筛选最佳的制作条件。以最佳的条件制备钽涂层试件，测试其力学性能；将人成骨细胞接种于钽涂层试件表面，培养3，7 d后进行扫描电镜观察。
结果与结论：①经过实验参数优化将最佳实验条件控制为：沉积温度1 050 ℃，氢气流量180 mL/min，氯气流量100 mL/min；②钽涂层试件的抗压强度为(61.4±3.2) MPa，屈服强度为(45.8±2.9) MPa，弹性模量为4.8 GPa；③扫描电镜显示培养3 d时，大量细胞黏附在钽涂层试件表面和多孔结构中，黏附的细胞充分铺展并伸出伪足，相互连接；培养7 d时，细胞覆盖在试件表面，在多孔结构中的细胞伸出伪足，互相交联跨过孔隙，并分泌基质包被微粒结构，逐步铺满孔隙；④结果表明，多孔碳化硅支架表面的钽涂层具有良好的力学性能与生物活性。
https://orcid.org/0000-0001-5228-3493 (马志杰) 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 骨, 材料, 支架, 钽金属, 成骨, 多孔, 碳化硅, 骨小梁, 影响因素

Abstract: BACKGROUND: Porous silicon carbide has excellent physical and chemical properties and can be used to repair complex shapes and long weight-bearing bone defects, but it has no biological activity and cannot promote bone remodeling and integration.
OBJECTIVE: A tantalum (Ta) coating was deposited on the surface of the porous silicon carbide scaffold to evaluate its biological activity.
METHODS: The chemical vapor deposition method was used to deposit a Ta coating on the surface of the porous silicon carbide scaffold. The optimal experimental parameters were explored by setting different gas reaction flow rates and temperatures. The Ta coating was prepared with the best experimental parameters to test the mechanical properties of porous Ta. Human osteoblasts were co-cultured with the Ta coating specimens, and were observed by scanning electron microscopy after 3 and 7 days of cultivation.
RESULTS AND CONCLUSION: (1) After the optimization of experimental parameters, the best experimental conditions were controlled as follows: deposition temperature of 1 050 °C, hydrogen flow rate of 180 mL/min and chlorine flow rate of 100 mL/min. (2) The compressive strength of the Ta coated specimen was (61.4±3.2) MPa; the yield strength was (45.8±2.9) MPa; and the elastic modulus was 4.8 GPa. (3) Scanning electron microscopy demonstrated that after 3 days of co-cultivation, a large number of cells adhered to the surface of the porous silicon carbide scaffold that is coated with Ta and the porous structure. Some of the cells protruded from the pseudopod and were connected to each other. After 7 days of co-cultivation, the cell protrusions fused to form a sheet covering the surface of the porous Ta. In the porous structure, the cells protruded from the pseudopods, cross-linked to each other across the pores, secreted in the matrix, and coated the microparticle structure, gradually spreading in the pores. (4) The results show that the Ta coating on the surface of porous silicon carbide scaffold has good mechanical properties and biological activity.

Key words: bone, material, scaffold, tantalum metal, osteogenesis, porous, silicon carbide, bone trabecula, influencing factor

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