中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (31): 5772-5776.doi: 10.3969/j.issn.2095-4344.2012.31.017

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

自体移植静脉模型中环氧化酶2表达及其与移植静脉狭窄的关系

黄健兵，胡丰庆，梅 举，丁芳宝   

  1. 上海交通大学医学院附属新华医院心胸外科，上海市 200092
  • 收稿日期:2011-11-25 修回日期:2012-12-19 出版日期:2012-07-29 发布日期:2012-07-29
  • 通讯作者: 丁芳宝，副教授，副主任医师，上海交通大学医学院附属新华医院心胸外科，上海市 200092
  • 作者简介:黄健兵☆，男，1979年生，江苏省海门市人，汉族，2008年解放军第二军医大学毕业，博士，主治医师，从事缺血性心脏病相关研究。 jb4480@163. com

Expression of cycloxygenase-2 in autologous vein grafts and its association with stenosis of grafted vein

Huang Jian-bing, Hu Feng-qing, Mei Ju, Ding Fang-bao   

  1. Department of Cardiothoracic Surgery, Affiliated Xinhua Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Received:2011-11-25 Revised:2012-12-19 Online:2012-07-29 Published:2012-07-29
  • Contact: Ding Fang-bao, Associate professor, Associate chief physician, Department of Cardiothoracic Surgery, Affiliated Xinhua Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • About author:Huang Jian-bing☆, Doctor, Attending physician, Department of Cardiothoracic Surgery, Affiliated Xinhua Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China jb4480@163.com

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摘要：

背景：环氧化酶2是与炎症反应密切相关的酶，其表达程度可能与移植静脉的狭窄相关。
目的：观察环氧化酶2在移植静脉中的表达情况，分析环氧化酶2的表达与移植静脉血管狭窄的相关性。
方法：实验分为2组，实验组在建立兔自体颈外静脉-颈总动脉移植模型后应用环氧化酶2抑制剂干预，对照组建模后正常喂养，于静脉移植后2，8，12周观察移植静脉血管壁增生情况及血管中环氧化酶2表达的差异。
结果与结论：术后各移植静脉均保持通畅。正常静脉无明显环氧化酶2表达，移植静脉环氧化酶2表达明显增高。实验组移植静脉中环氧化酶2 mRNA表达较对照组低，移植静脉内膜及中膜增厚程度也低于对照组。结果表明，环氧化酶2在移植静脉中出现明显的表达增高，抑制移植静脉中环氧化酶2的表达可以减缓移植静脉血管壁的增生。

关键词: 环氧化酶2, 移植静脉, 再狭窄, 动物模型, 内膜

Abstract:

BACKGROUND: Cycloxygenase-2 is the enzyme closely related to inflammatory reaction. Its expression may be associated with stenosis of grafted vein.
OBJECTIVE: To study the cycloxygenase-2 expression in the transplanted vein, and to investigate the relevance between cycloxygenase-2 expression and stenosis of grafted vein.
METHODS: The experiment was divided into two groups. The autogenously vein graft rabbit models were established in two groups, and the models in the experimental group were intervened by cycloxygenase-2 inhibitors and the models in the control group were fed normally after modeling. The cycloxygenase-2 expression and the proliferation of venous wall in the transplanted models were observed after vein transplantation.
RESULTS AND CONCLUSION: All the autogenously graft vein remain patency. The cycloxygenase-2 expression was not obvious in the normal vein and increased significantly in the transplanted vein. The cycloxygenase-2 mRNA expression in the transplanted vein of the experimental group was lower than that of the control group, the thickening degree of the intimal and medial of transplanted vein in the experimental group was lower than that in the control group. Cycloxygenase-2 expression increased significantly in the transplanted vein, and inhibition the cycloxygenase-2 expression can slow down hyperplasia of transplanted vein.

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