中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (27): 4414-4419.doi: 10.3969/j.issn.2095-4344.1395

• 组织构建综述 tissue construction review • 上一篇    下一篇

银屑病体内外模型分类与研究展望

徐永跃1,陈海明1,邓静文1,王茂杰1,2,闫玉红1,黄闰月1,3,卢传坚1,3   

  1.  (1广州中医药大学第二附属医院(广东省中医院),广东省广州市  510120;2荷兰乌特勒支大学医学中心,荷兰乌特勒支市;3广东省中医院中医证候临床研究重点实验室,广东省广州市  510006)
  • 收稿日期:2019-03-29 出版日期:2019-09-28 发布日期:2019-09-28
  • 通讯作者: 卢传坚,博士,教授,主任医师,广州中医药大学第二附属医院(广东省中医院),广东省广州市 510120;广东省中医院中医证候临床研究重点实验室,广东省广州市 510006
  • 作者简介:徐永跃,男,1991年生,广东省茂名市人,汉族,2017年广州中医药大学毕业,硕士,研究实习员,主要从事中医药调节免疫相关疾病的研究。
  • 基金资助:

    国家自然科学基金面上项目(81873302),项目负责人:卢传坚;广东省中医院中医药科学技术研究专项资助(YN2018ZD01),项目负责人:卢传坚;广东省中医院中医药科学技术研究专项资助(YN2019QJ09),项目负责人:徐永跃

Classification and research prospect of in vitro and in vivo models of psoriasis

Xu Yongyue1, Chen Haiming1, Deng Jingwen1, Wang Maojie1, 2, Yan Yuhong1, Huang Runyue1, 3, Lu Chuanjian1, 3   

  1.  (1the Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Traditional Chinese Medicine), Guangzhou 510120, Guangdong Province, China; 2Utrecht University Medical Center, Utrecht, Holland; 3Key Laboratory of Traditional Chinese Medicine Syndrome Clinical Research, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, Guangdong Province, China)
  • Received:2019-03-29 Online:2019-09-28 Published:2019-09-28
  • Contact: Lu Chuanjian, MD, Professor, Chief physician, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Traditional Chinese Medicine), Guangzhou 510120, Guangdong Province, China; Key Laboratory of Traditional Chinese Medicine Syndrome Clinical Research, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, Guangdong Province, China
  • About author:Xu Yongyue, Master, Researcher, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Traditional Chinese Medicine), Guangzhou 510120, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China (General Program), No.81873302 (to LCJ); the Project of Guangdong Province Traditional Chinese Medical Hospital (Key Laboratory Project), No. YN2018ZD01 (to LCJ) and YN2019QJ09 (to XYY)

摘要:

文章快速阅读:

文题释义:

回交实验:是子一代与它两个亲本中的任一个亲本进行杂交的一种实验方法。回交实验对于性状遗传的研究有重要的意义,特别是使用隐性亲本进行回交,是检验其子一代基因型的有效方法。
中医病证结合动物模型:一种包含了特定的现代医学疾病元素与中医“证”的元素的复合动物模型。为了让动物模型更加体现中医的证候特色,相关领域的学者在单纯的疾病动物的研究模型基础上,加入中医的“证”的相应内容,创建“病证结合”的动物模型。目前病证结合动物模型研究较多,尚有待规范化。
摘要
背景:
银屑病体内外模型是理解银屑病发病过程的重要途径,现有的动物模型虽然都能够部分地模拟银屑病的病理表现,但缺乏代表性。
目的:归纳和总结目前的银屑病动物模型和细胞模型的分类及研究进展,为研究者选用合适的银屑病模型提供有益参考。
方法:使用PubMed数据库、中国知网(CNKI)数据库、万方数据库,分别以“银屑病”、“动物模型”为中文主题词,或以“psoriasis”和“animal model”为英文主题词进行检索,经过纳入文献筛选后,最终得到51篇文献。
结果与结论:把纳入研究的文献按照动物的造模方法进行归纳,总结出银屑病所用的动物模型主要包括基因工程小鼠模型、自发银屑病样小鼠模型、外源诱发银屑病样模型、异种移植模型和中医病证结合动物模型。各种银屑病动物模型都在一定程度上模拟银屑病的病理表现,但仍存在发病机制差异和种属差异性的局限性。细胞模型可以揭示银屑病角质形成细胞或其他细胞类型的生物学行为改变及其相互作用。研究者可以结合研究目的和自身实验条件,选用最合适的银屑病体内外模型。

关键词: 银屑病, 动物模型, 细胞模型, 分类, 白细胞介素, 基因工程

Abstract:

BACKGROUND: In vivo and in vitro models of psoriasis are an important way to understand the pathogenesis. Although existing animal models can partially simulate the pathological manifestations of psoriasis, they are not representative.
OBJECTIVE: To summarize the classification and research progress of animal and cell models of psoriasis, and to provide beneficial references for researchers to select appropriate models of psoriasis.
METHODS: PubMed, CNKI and WanFang databases were retrieved with the keywords of “psoriasis and animal model” in English and Chinese, respectively. After screening the literature, 51 eligible articles were finally included.
RESULTS AND CONCLUSION: According to the animal modeling method, the literature included in the study was summarized. The animal models used for psoriasis included genetic engineering mouse model, spontaneous psoriasis mouse model, exogenous induced psoriasis model, xenotransplantation model and traditional Chinese medicine disease-syndrome combination animal model. All kinds of animal models of psoriasis simulate the pathological manifestations of psoriasis to a certain extent, but there are still limitations of differences in pathogenesis and species. Cell models can reveal changes in biological behaviors and interactions of keratinocytes or other cell types in psoriasis. Researchers can choose the most suitable model of psoriasis in vivo and in vitro according to their research purposes and experimental condition.

Key words: psoriasis, animal model, cell model, classification, interleukin, genetic engineering

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