中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (27): 4275-4279.doi: 10.3969/j.issn.2095-4344.1372

• 骨组织构建 bone tissue construction • 上一篇    下一篇

番茄红素对氧化应激状态下成骨细胞增殖和功能的影响

荣  慧1，薛文利2，龙燕鸣2，谢梦生2，曹  勇2，李晓捷2   

  1. (1广西中医药大学第一附属医院，广西壮族自治区南宁市  530023；2广西医科大学口腔医学院、广西口腔颌面修复与重建研究自治区级重点实验室、广西颅颌面畸形临床医学研究中心、颌面外科疾病诊治研究重点实验室(广西高校重点实验室)，广西壮族自治区南宁市  530021)
  • 收稿日期:2019-04-25 出版日期:2019-09-28 发布日期:2019-09-28
  • 通讯作者: 曹勇，硕士，副主任医师，广西医科大学口腔医学院、广西口腔颌面修复与重建研究自治区级重点实验室、广西颅颌面畸形临床医学研究中心、颌面外科疾病诊治研究重点实验室(广西高校重点实验室)，广西壮族自治区南宁市 530021 并列通讯作者：李晓捷，博士，副教授，广西医科大学口腔医学院、广西口腔颌面修复与重建研究自治区级重点实验室、广西颅颌面畸形临床医学研究中心、颌面外科疾病诊治研究重点实验室(广西高校重点实验室)，广西壮族自治区南宁市 530021
  • 作者简介:荣慧，女，1983年生，江西省上栗县人，2017年广西医科大学毕业，硕士，主治医师，主要从事口腔修复学临床及基础研究。
  • 基金资助:

    广西自然科学基金(2013GXNSFBA019162)，项目负责人：李晓捷；广西高校中青年教师基础能力提升项目(2017KY0092)，项目负责人：李晓捷；广西医科大学青年科学基金(GXMUYF201634)，项目负责人：曹勇

Effect of lycopene on proliferation and function of osteoblasts under oxidative stress

Rong Hui1, Xue Wenli2, Long Yanming2, Xie Mengsheng2, Cao Yong2, Li Xiaojie2   

  1.  (1the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China; 2College of Stomatology, Guangxi Medical University, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Nanning 530021, Guangxi Zhuang Autonomous Region, China)
  • Received:2019-04-25 Online:2019-09-28 Published:2019-09-28
  • Contact: Cao Yong, Master, Associate chief physician, College of Stomatology, Guangxi Medical University, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Nanning 530021, Guangxi Zhuang Autonomous Region, China Corresponding author: Li Xiaojie, MD, Associate professor, College of Stomatology, Guangxi Medical University, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Rong Hui, Master, Attending physician, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2013GXNSFBA019162 (to LXJ); the Basic Ability Promotion Project of Young and Middle-Aged Teachers in Guangxi Universities, No. 2017KY0092 (to LXJ); the Youth Science Foundation of Guangxi Medical University, No. GXMUYF201634 (to CY)

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摘要：

文章快速阅读：

文题释义：
氧化应激：是指在疾病或者激素水平改变时，体内氧化与抗氧化平衡失调，导致体内活性氧含量增加的一种状态。氧化应激状态通过体内一系列的细胞信号传导来影响骨代谢，常表现为破骨细胞异常活跃而成骨细胞功能受限。
番茄红素：番茄红素是一种广泛存在于西红柿、西瓜、番石榴等植物的天然色素，番茄红素属于类胡萝卜素，是最强天然抗氧化剂之一，其对单个氧分子的清除率比其他类胡萝卜素高100倍。局部及系统性应用番茄红素可以有效拮抗氧化应激对细胞的损伤作用。
摘要
背景：氧化应激是骨代谢的重要影响因素，多种疾病引起的体内氧化应激状态导致骨代谢紊乱，通过抗氧化制剂调整体内骨代谢水平对于预防骨质丢失具有重要意义。
目的：探讨番茄红素对氧化应激状态下成骨细胞增殖、分化及矿化能力的影响。
方法：动物实验方案经广西医科大学动物实验伦理委员会批准(批准号：201601007)。采用酶消化法获取SD乳鼠颅盖骨细胞，培养及鉴定为成骨细胞，取第3代细胞进行实验。实验分为H2O2组(含100 μmol/L双氧水的培养液培养24 h后，更换为体积分数10%FBS培养)、对照组(体积分数10%FBS培养)及10，102，103，104 nmol/L番茄红素组(含不同浓度的番茄红素培养液培养24 h后，换100 μmol/L双氧水的培养液培养24 h后，更换为体积分数10%FBS培养)，共培养21 d。观察不同浓度番茄红素对双氧水模拟的氧化应激状态下成骨细胞增殖、分化及矿化指标的影响。
结果与结论：①10-103 nmol/L的番茄红素预防性用药组在培养期内细胞增殖量均高于H2O2组(P < 0.05)，104 nmol/L番茄红素组在培养的第3，5，7天细胞增殖量明显低于其他组(P < 0.05)；②10-103 nmol/L的番茄红素预防性用药组在培养期内细胞碱性磷酸酶活性均高于H2O2组(P < 0.05)；③10-103 nmol/L的番茄红素预防性用药组在培养期内细胞矿化能力均高于H2O2组(P < 0.05)，在此番茄红素浓度范围，浓度越高矿化能力的保护作用越强(P < 0.05)；④结果说明，番茄红素预防性用药可以降低氧化应激状态对成骨细胞增殖、分化及矿化的影响。

关键词: 成骨细胞, 番茄红素, 增殖, 分化, 氧化应激, 矿化, 碱性磷酸酶

Abstract:

BACKGROUND: Oxidative stress is an important factor for bone metabolism, and oxidative stress in vivo caused by various diseases can lead to bone metabolism disorder. Regulating bone metabolism level in vivo by antioxidant is of great significance for preventing bone mass loss.
OBJECTIVE: To investigate the effect of lycopene on the proliferation, differentiation and mineralization of osteoblasts under oxidative stress.
METHODS: The study was approved by the Experimental Animal Ethics Committee of Guangxi Medical University, approval number: 201601007. The osteoblasts were obtained by enzymatic digestion in the calvarial cap of Sprague-Dawley neonatal mice, and were cultured and identified. The passage 3 cells were used for experiment. There were H2O2 group (24-hour culture in the medium containing 100 μmol/L H2O2, and then cultured in the 10% FBS), control group (cultured in the 10% FBS), 10, 102, 103 and 104 nmol/L lycopene groups (24-hour culture in the medium containing different concentrations of lycopene, 24-hour culture in the medium containing 100 μmol/L H2O2, and then cultured in the 10% FBS). The cells in each group were cultured for 21 days. The effects of different concentrations of lycopene on the proliferation, differentiation and mineralization of osteoblasts under the simulated oxidative stress of hydrogen peroxide were detected.
RESULTS AND CONCLUSION: (1) The cell proliferation in the 10-103 nmol/L lycopene prophylactic application groups was significantly higher than that in the H2O2 group (P < 0.05). The cell proliferation in the 104 nmol/L lycopene group at 3, 5 and 7 days of culture was significantly lower than that in the other groups (P < 0.05). (2) The alkaline phosphatase activity of 10-103 nmol/L lycopene prophylactic application groups was significantly higher than that in the H2O2 group (P < 0.05). (3) The 10-103 nmol/L lycopene prophylactic application groups had significantly higher cell mineralization ability than the H2O2 group (P < 0.05), and the higher the concentration of lycopene, the stronger the protective effect of the mineralization ability to the cell (P < 0.05). (4) To conclude, lycopene prophylactic application can reduce the effect of oxidative stress on proliferation, differentiation and mineralization of osteoblasts.

Key words: osteoblasts, lycopene, proliferation, differentiation, oxidative stress, mineralization, alkaline phosphatase

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