中国组织工程研究

中国组织工程研究

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

烧结法制备掺镁透钙磷石骨水泥

贾婉萍1，董 伟2，彭宏峰2，徐艳丽2，王红美1，梁立硕1，戚孟春2，梁永强1   

  1. 华北理工大学口腔医学院，1正畸科，2颌面外科，河北省唐山市 063000
  • 收稿日期:2018-01-13 出版日期:2018-08-08 发布日期:2018-08-08
  • 通讯作者: 梁永强，博士，副教授，华北理工大学口腔医学院正畸科，河北省唐山市 063000
  • 作者简介:贾婉萍，女，1989年生，河北省邯郸市人，汉族，华北理工大学在读硕士，医师，主要从事口腔正畸学研究。
  • 基金资助:

     国家自然科学基金(81270965)；河北省教育厅重点项目(ZD2015016)

Preparation of magnesium-doped brushite by sintering method

Jia Wan-ping1, Dong Wei2, Peng Hong-feng2, Xu Yan-li2, Wang Hong-mei1, Liang Li-shuo1, Qi Meng-chun2, Liang Yong-qiang1   

  1. 1Department of Orthodontics, 2Department of Maxillofacial Surgery, School of Stomatology, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • Received:2018-01-13 Online:2018-08-08 Published:2018-08-08
  • Contact: Liang Yong-qiang, M.D., Associate professor, Department of Orthodontics, School of Stomatology, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • About author:Jia Wan-ping, Master candidate, Physician, Department of Orthodontics, School of Stomatology, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81270965; the Major Project of Hebei Provincial Educational Department, No. ZD2015016

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摘要：

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文题释义：
掺镁透钙磷石：透钙磷石为羟基磷灰石的前体物质，它可由β-磷酸钙与一水磷酸二氢钙水化而成，极易降解，具有较好的生物相容性和可注射等特性。为优化透钙磷石的性能，用镁离子取代透钙磷石中的钙离子而生成的材料称为掺镁透钙磷石。
生物相容性：指植入材料与生物体之间相互作用后产生的各种生物、物理、化学等反应的一种概念，分为体外生物相容性和体内生物相容性。一般地讲，就是材料植入人体后与人体的相容程度，也就是说是否会对人体组织造成毒害作用，是国家医用材料一项重要的标准。
 
 
背景：透钙磷石作为可吸收的磷酸钙骨水泥和骨替代植入材料，在性质特征上存在一些不足，学者们尝试对透钙磷石进行改性，以期能增强其机械性能，延长固化时间，提高成骨作用。
目的：制备掺镁透钙磷石骨水泥，通过理化性能、生物活性和修复骨缺损能力检测掺镁透钙磷石骨水泥作为骨替代材料的可行性。
方法：采用烧结法将镁离子引入β-磷酸三钙，设置Mg/(Mg+Ca)摩尔百分比分别为0%、6.67%、26.67%，制备掺镁透钙磷石骨水泥，采用扫描电子显微镜观察骨水泥的形态，万能材料实验机检测骨水泥的抗压强度。将0%、6.67%、26.67%掺镁透钙磷石骨水泥浸提液分别加入兔抗凝血中，检测溶血率。制备24只家兔双侧桡骨骨缺损模型，分4组干预，其中3组骨缺损处分别植入0%、6.67%、26.67%掺镁透钙磷石骨水泥，空白组不做任何处理，植入后4，8周进行X射线检查。

结果与结论：①扫描电镜显示，0%掺镁透钙磷石骨水泥为堆积紧密的板片状和少量颗粒状，孔隙较少；6.67%掺镁透钙磷石骨水泥呈不规则团块状和短棒状；26.67%掺镁透钙磷石骨水泥为团块状、圆球状和颗粒状等结构；②0%、6.67%、26.67%掺镁透钙磷石骨水泥的抗压强度分别为31.99，26.38，24.44 MPa；③所有透钙磷石骨水泥的溶血率均小于5%；④X射线显示，植入4周时，空白组缺损区边缘规则，无新骨形成；掺镁0%组骨水泥周围溶解，与骨缺损交界处有少量高密度影像；掺镁6.67%组骨水泥大部分溶解，大量新骨形成；26.67%掺镁组骨水泥周围溶解，中央呈高密度团块，新生骨量较少。植入8周时，空白组两断端处有新骨沉积影像；未掺镁组新生骨呈楔形堆积；掺镁6.67%组骨缺损处基本充满新骨，骨皮质连续；掺镁26.67%组新骨呈桥接式链接骨缺损断端，塑性差；⑤结果表明掺镁6.67%透钙磷石骨水泥具有良好的机械性能与成骨效果。

ORCID: 0000-0001-9495-5294(贾婉萍)

关键词: 骨材料, 烧结法, 镁, β-磷酸三钙, 掺镁透钙磷石, 生物材料, 桡骨骨缺损, 成骨效果, 兔, 骨替代材料, 国家自然科学基金

Abstract:

BACKGROUND: As an absorbable calcium phosphate cement and bone substitute implant, calcium phosphate stone has become the focus of bone tissue engineering research, but some deficiencies in its properties have greatly limited its clinical application. The scholars try to add some elements to modify the calcium phosphate stone, in order to enhance the mechanical properties, prolong the curing time and improve the osteogenesis.

OBJECTIVE: To make magnesium-doped brushite by adding magnesium ions to beta-tricalcium phosphate with sintering method and to detect the feasibility of magnesium-doped brushite as a bone substitute by detecting physical and chemical properties and ability of repairing bone defects.
METHODS: Magnesium-doped brushite in different molar ratios of Mg/(Mg+Ca) (0%, 6.67% and 26.67%) was prepared using sintering method. Morphological structure and compressive strength of the prepared bone cement were detected using scanning electron microscope and universal material test machine, respectively. Magnesium-doped brushite extracts (0%, 6.67%, 26.67%) were added into rabbit anticoagulant to perform a hemolytic test. Twenty-four rabbits were used to make bilateral tibial bone defect models, and then rabbit models were divided into four groups: three groups were implanted with 0%, 6.67%, and 26.67% magnesium-doped brushite bone cement, and the blank group received no treatment. X-ray examinations were performed at 4 and 8 weeks after implantation.

RESULTS AND CONCLUSION: Under the scanning electron microscopy, the 0% magnesium-doped brushite cement had a tightly packed plate-like shape and a small amount of granules with less pores; the 6.67% magnesium-doped brushite cement presented with irregular lumps and short rods; and the 26.67% magnesium-doped brushite cement was lumpy, spherical and granular. The magnesium-doped brushite cements (0%, 6.67%, 26.67%) had compressive strengths of 31.99, 26.38, 24.44 MPa, respectively. The hemolysis rate of all the brushite bone cements was less than 5%. X-ray results showed that at the 4th week after implantation, the defect margin in the blank group was ruled without new bone formation; in the 0% magnesium-doped brushite group, the bone cement was dissolved at the margin, and a few high-density shadows were detected at the junction between bone defects and the cement; in the 6.67% magnesium-doped brushite group, the bone cement was mostly dissolved and there were a large amount of new bone tissues; in the 26.67% magnesium-doped brushite group, the bone cement was dissolved at the margin, and there were high-density shadows in the center of the bone cement with less new bone formation. At 8 weeks after implantation, there were new bone deposits at the two ends of the blank group; the new bone was wedge-shaped in the 0% magnesium-doped brushite group; the bone defect in the 0% magnesium-doped brushite group was basically filled with new bone tissues, and the bone cortex was continuous; and in the 26.67% magnesium-doped brushite group, the new bone served as a bridge to link the broken bone ends with poor plasticity. These findings indicate that 6.67% magnesium-doped brushite cement has good mechanical properties and osteogenic effects.

Key words: Magnesium, Calcium Phosphates, Tissue Engineering

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