中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (18): 2849-2854.doi: 10.3969/j.issn.2095-4344.0703

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

牙科修复材料苯扎氯铵表面活性剂对5种常见口腔致病菌的体外抑菌效果

代 静，陈燕斌，陈 珊，任 静，李昆蔓，杨军英   

  1. 中山大学附属第一医院口腔科，广东省广州市 510080
  • 收稿日期:2017-11-02 出版日期:2018-06-28 发布日期:2018-06-28
  • 通讯作者: 杨军英，硕士，教授，硕士生导师，中山大学附属第一医院口腔科，广东省广州市 510080
  • 作者简介:代静，女，1991年生，安徽省阜阳市人，汉族，中山大学附属第一医院口腔科在读硕士，主要从事口腔内科方面的研究。
  • 基金资助:

    横向课题(K0601161)：苯扎氯铵对口腔疾病常见致病菌的体外抑菌实验

In vitro antibacterial effect of benzalkonium chloride on five common oral pathogens

Dai Jing, Chen Yan-bin, Chen Shan, Ren Jing, Li Kun-man, Yang Jun-ying   

  1. Department of Stomatology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Received:2017-11-02 Online:2018-06-28 Published:2018-06-28
  • Contact: Yang Jun-ying, Master, Professor, Master’s supervisor, Department of Stomatology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • About author:Dai Jing, Master candidate, Department of Stomatology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Supported by:

    A Transverse Issue: In Vitro Bacteriostasis Experiment of Benzalkonium Chloride on Common Pathogens of Oral Diseases, No. K0601161

PDF

228

摘要：

文章快速阅读：

 
文题释义：
苯扎氯铵：常用的消毒防腐药，是一种季铵盐类阳离子表面活性剂，在水溶液中显中性或弱碱性反应，振摇时产生大量泡沫，可离解成阳离子活性基团，能改变细菌胞浆膜通透性，使菌体胞浆物质外渗导致细菌死亡，具有广谱、高效的杀菌能力，在医疗手术时被广泛用于皮肤和手术器械的消毒，也被广泛用于杀菌、消毒、防腐等方面。
口腔致病菌：口腔内的微生物以细菌为主，目前已知至少700余种细菌，是人类与微生物长期共存进化过程中形成的最复杂的微生物群，正常情况下对宿主无不良影响，当菌群失调时，某些具有显著毒力或致病性的致病菌过度繁殖，能通过多种机制干扰宿主防御能力，具有引发疾病的潜能。如牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、变形链球菌、粪肠球菌等均是常见的口腔致病菌。 

 

背景：目前，苯扎氯铵已被用于牙科修复材料中，能增强材料的抗菌性并作用持久。然而，目前尚缺乏苯扎氯铵与牙髓牙周感染相关细菌作用的研究。

目的：研究苯扎氯铵对口腔主要致病菌的体外抑菌效果。
方法：采用琼脂扩散法比较0.1%苯扎氯铵、体积分数3%双氧水、生理盐水与5.25%次氯酸钠对牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、变形链球菌、粪肠球菌的体外抑菌效果，采用试管稀释法测定苯扎氯铵对上述细菌的最低抑菌浓度。

结果与结论：①对牙龈卟啉单胞菌与中间普氏菌，0.1%苯扎氯铵表现出了较强的抑菌效果，抑菌效果弱于5.25%次氯酸钠(P < 0.05)；对牙龈卟啉单胞菌，0.1%苯扎氯铵的抑菌效果与体积分数3%双氧水无差异(P> 0.05)；对中间普氏菌，0.1%苯扎氯铵的抑菌效果优于体积分数3%双氧水(P < 0.05)；对伴放线放线杆菌和变形链球菌，0.1%苯扎氯铵的抑菌效果均优于体积分数3%双氧水(P < 0.05)，与5.25%次氯酸钠无差异   (P > 0.05)；对粪肠球菌，0.1%苯扎氯铵的抑菌效果优于体积分数3%双氧水(P < 0.05)，弱于5.25%次氯酸钠(P< 0.05)；体积分数3%双氧水对粪肠球菌不产生抑菌环；②0.1%苯扎氯铵对牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、变形链球菌、粪肠球菌的最低抑菌浓度分别为16，2，4，2，4 mg/L；③结果表明，0.1%苯扎氯铵对牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、变形链球菌、粪肠球菌具有较强的抑菌效果。

ORCID: 0000-0002-3805-603X(杨军英) 

关键词: 口腔材料, 苯扎氯铵溶液, 牙龈卟啉单胞菌, 中间普氏菌, 伴放线放线杆菌, 变形链球菌, 粪肠球菌, 琼脂扩散法, 生物材料

Abstract:

BACKGROUND: Benzalkonium chloride has been used in dental restorative materials to enhance the long-lasting antibacterial properties of materials.

OBJECTIVE: To evaluate the antibacterial activity of benzalkonium chloride on oral common pathogenic bacteria in vitro.
METHODS: The agar diffusion method was used to determine the inhibitory effects of antibacterial agents, 0.1% benzalkonium chloride, 3% H2O2 and 5.25% sodium hypochlorite, on five kind of oral pathogens, Porphyromonas gingivalis (P.g), Prevotella intermedia (P.i), Actinobacillus actionmycemcomitans (A.a), Streptococcus mutans (S.m) and Enterococcus faecalis (E.f). The tube dilution method was used to detect the minimal inhibitory concentration of benzalkonium chloride against the five bacteria mentioned above.
RESULTS AND CONCLUSION: The antibacterial effect of 0.1% benzalkonium chloride on P.g was not significantly different from that of 3% hydrogen peroxide (P > 0.05), while 0.1% benzalkonium chloride showed better effect on P.i than 3% hydrogen peroxide (P < 0.05). On P.g and P.i, the antibacterial effect of 0.1% benzalkonium chloride was worse than that of 5.25% sodium hypochloritethe (P < 0.05). The antibacterial effect of 0.1% benzalkonium chloride on A.a and S.m was better than that of 3% hydrogen peroxide (P < 0.05), and similar to that of 5.25% sodium hypochlorite (P > 0.05). The antibacterial effect of 0.1% benzalkonium chloride on E.f was better than that of 3% hydrogen peroxide (P < 0.05), but worse than that of 5.25% sodium hypochlorite (P < 0.05). The minimal inhibitory concentration of benzalkonium chloride to P.g, P.i, A.a, S.m, E.f was 16, 2, 4, 2, 4 mg/L, respectively. To conclude, 0.1% benzalkonium chloride has strong antibacterial effects on P.g, P.i, A.a, S.m and E.f. 

Key words: Dental Materials, Benzalkonium Compounds, Microbial Sensitivity Tests, Tissue Engineering

中图分类号: