中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (21): 3393-3400.doi: 10.3969/j.issn.2095-4344.0516

• 干细胞综述 stem cell review • 上一篇    下一篇

细胞微囊泡/外泌体在肺部疾病中的应用

罗 登1,冉文卓2,叶迎春1,高 凌1   

  1. 1武汉大学人民医院东院内分泌科,湖北省武汉市 430000;2武汉市第一医院中心实验室,湖北省武汉市 430023
  • 修回日期:2018-03-27 出版日期:2018-07-28 发布日期:2018-07-28
  • 通讯作者: 叶迎春,硕士,主治医师,武汉大学人民医院东院内分泌科,湖北省武汉市 430000
  • 作者简介:罗登,男,1984年生,湖北省钟祥市人,汉族,2015年上海交通大学医学院毕业,博士,主治医师,主要从事内分泌与代谢病、干细胞与组织再生工程研究。
  • 基金资助:

    武汉大学自主科研资助项目(2042017kf0084);湖北省自然科学基金项目(2018CFB230)

Microvesicles and exosomes in the lung diseases

Luo Deng1, Ran Wen-zhuo2, Ye Ying-chun1, Gao Ling1   

  1. 1Department of Endocrinology, Eastern Branch of Renmin Hospital, Wuhan University, Wuhan 430000, Hubei Province, China; 2Clinical Laboratory, Wuhan No. 1 Hospital, Wuhan 430023, Hubei Province, China
  • Revised:2018-03-27 Online:2018-07-28 Published:2018-07-28
  • Contact: Ye Ying-chun, Master, Attending physician, Department of Endocrinology, Eastern Branch of Renmin Hospital, Wuhan University, Wuhan 430000, Hubei Province, China
  • About author:Luo Deng, Ph.D., Attending physician, Department of Endocrinology, Eastern Branch of Renmin Hospital, Wuhan University, Wuhan 430000, Hubei Province, China
  • Supported by:

    Independent Research Project of Wuhan University of China, No. 2042017kf0084; Natural Science Foundation of Hubei Province, No. 2018CFB230

摘要:

文章快速阅读:

文题释义:
细胞微囊泡/外泌体:
其源于细胞膜,具有与母细胞膜类似的组成成分,在正常及特定条件下皆可释放,因携带生物活性物质可作为细胞间信号传递的分子,近年来受到广泛关注,成为研究热点。
外泌体与微粒/微囊泡的异同:两者都属于细胞外囊泡的范畴,虽然功能类似,但有差异。外泌体是由细胞内的多泡小体与细胞膜融合后以外分泌的形式释放到细胞外,直径为40-100 nm;微囊泡是细胞激活、损伤或凋亡后从细胞膜脱落的小囊泡,直径为100-2 000 nm。

 

摘要
背景:
相关研究已表明微囊泡/外泌体可以利用其在细胞间信息传递的优势改善受体细胞的功能、保护损伤组织、提示疾病发生发展,有望成为多种肺病防治的新策略。
目的:探讨微囊泡或外泌体在肺病中的应用进展。
方法:在标题和摘要中,以“exosome, microvesicle, microparticle, extracellular vesicles, lung, pulmonary, pneumonic, pulmonary, pulmonic”或“细胞微囊泡,外泌体,肺”为检索词,检索PubMed数据库、中国期刊全文数据库(CNKI)和万方数据库中2007年1月至2017年12月关于细胞来源微囊泡/外泌体应用于肺脏疾病治疗的中英文文献,排除重复及不相关研究。
结果与结论:①根据纳入和排除标准,最终纳入68篇文献进行整理和进一步分析;②微囊泡、外泌体因直径小可避免被吞噬,能维持所携带的mRNA,microRNA和蛋白的活性,便于与周围细胞组织进行信息交流,可作为参与肺脏生理和病理微环境的新型旁分泌介质,在肺脏疾病中的应用颇具前景;③有些微囊泡、外泌体含有肺病的特定标志物,可作为诊断疾病或判断预后的依据;④有些微囊泡、外泌体携带的生物活性物质有促进血管生成、促细胞增殖、迁移的作用,或可作为调控肺病发生发展的潜在靶点;⑤有些微囊泡、外泌体应用于实验动物呼吸系统疾病模型,可减轻水肿,减少促炎因子分泌,促进损伤修复,较细胞移植疗法更具优势;⑥目前临床上对于微囊泡、外泌体的应用还仅停留在肺病的诊断和预后判断,用于调控干预肺病发展的微囊泡、外泌体仍处在实验室阶段,还需大量基础实验来明确,包括用于治疗肺病的微囊泡、外泌体来源,活性成分,用量疗程等系列问题。

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID:
0000-0003-2928-779X(罗登)

关键词: 微囊泡, 外泌体, 慢性阻塞性肺病, 肺栓塞, 肺损伤, 弥漫性实质性肺病, 肺动脉高压, 哮喘, 肺癌, 干细胞, 湖北省自然科学基金

Abstract:

BACKGROUND: Microvesicles/exosomes as mediators for intercellular transfer can improve function of recipient cells, protect damaged tissues and predict the development of diseases. They have the potential to become a new treatment strategy for a variety of lung diseases.
OBJECTIVE: To explore the application of microvesicles/exosomes in lung diseases.
METHODS: The authors retrieved PubMed, CNKI and WanFang databases for relative articles addressing the application of stem cell-derived microvesicles/exosomes in lung diseases published from January 2007 to December 2017. The keywords were “exosome, microvesicle, microparticle, extracellular vesicles, lung, pulmonary, pneumonic, pulmonary, pulmonic” in English and Chinese, respectively. The irrelative and repetitive literatures were excluded.
RESULTS AND CONCLUSION: Finally 68 eligible literatures were enrolled based on the inclusion and exclusion criteria. Microvesicles/ exosomes cannot be phagocytosed because of their small diameter. They play a pivotal role in cell-to-cell communication depending on mRNAs, microRNAs and proteins within them. As a new paracrine protein involved in the physiological and pathological microenvironment, microvesicles/exosomes may be the promising strategy for the diagnosis or prognostic prediction of lung diseases. Microvesicles/exosomes carrying bioactive substances promote angiogenesis, cell proliferation and migration. They are deemed to be a potential target for the modulation of lung diseases. In addition, microvesicles/exosomes can relieve edema, reduce pro-inflammatory cytokine secretion and promote injury repair in animal respiratory disease models. Compared to the traditional therapy, they have more advantages in diagnosis and prognosis of lung diseases. However, the use of microvesicles/exosomes is still at its initial stage. A series of problems, including sources, active constituent and dosage of microvesicles/exosomes, are needed to be resolved in the future.

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Exosomes, Lung Diseases, Review, Tissue Engineering

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