中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (5): 815-820.doi: 10.3969/j.issn.2095-4344.0456

• 干细胞综述 stem cell review • 上一篇    

细胞外基质硬度和厚度对细胞生长的影响

李 珊1，2，刘晓艺1，2，孙 艳1，2，赵 峰1，何静雯1   

  1. 1北京航空航天大学生物与医学工程学院，北京市 100191；2中国科学院上海微系统与信息技术研究所，传感技术联合国家重点实验室，上海市 200050
  • 修回日期:2017-09-14 出版日期:2018-02-18 发布日期:2018-02-18
  • 通讯作者: 孙艳，博士，硕士生导师，副教授，北京航空航天大学生物与医学工程学院，北京市 100191Sun
  • 作者简介:李珊，女，1992年生，河北省任丘市人，汉族，北京航空航天大学生物与医学工程学院在读硕士，主要从事组织工程方向的研究。
  • 基金资助:

    国家自然科学基金(31470942)；111 Project 345(B13003)

Effects of extracellular matrix stiffness and thickness on cell growth

Li Shan1, 2, Liu Xiao-yi1, 2, Sun Yan1, 2, Zhao Feng1, He Jing-wen1   

  1. 1School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; 2State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China
  • Revised:2017-09-14 Online:2018-02-18 Published:2018-02-18
  • Contact: Yan, M.D., Master’s supervisor, Associate professor, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China
  • About author:Li Shan, Studying for master’s degree, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31470942; 111 Project 345, No. B13003

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摘要：

文章快速阅读：

文题释义：
弹性模量：是对于基质硬度的一种定量性描述。弹性模量越大表明基质的硬度越大，基质越硬；弹性模量越小代表基质硬度越小，基质越软。基质弹性模量的大小与基质材料组成成分、温度等多种因素有关，当研究基质厚度对细胞生长的影响时，需保证基质的弹性模量固定。
细胞外微环境：是细胞生存的环境，一个稳定的细胞外微环境能够保证细胞的铺展、黏附、增殖和分化等生命活动正常进行。文中研究的基质硬度和厚度都属于细胞外微环境的重要力学性质，除此之外还有交联程度等其他性质。

 

摘要
背景：细胞外基质的力学性质会影响细胞的铺展、增殖和分化，对于组织工程和再生医学研究及应用均有极其重要的意义。
目的：总结近年来有关基质力学性质对细胞生长的影响，并分析基质厚度和硬度之间是否存在关系，为体外构建功能性组织提供实验方法参考。
方法：作者检索1988至2016年百链云数据库和PubMed数据库，选择与基质力学性质对细胞生长影响有关的文献，进行系统整理、总结归纳和分析。
结果与结论：检索到文章254篇，最终纳入43篇。通过总结及分析发现黏附在基质上的细胞结构和功能与其所处的外环境有很大的关系。体外模拟实验表明基质硬度的增大会使得细胞的形态发生变化，铺展面积增大，增殖水平提高，同样也会对细胞的分化有影响。当基质厚度在一定范围内时，细胞可以感知到基质厚度的变化。当基质厚度超过一定范围，细胞则感知不到基质的厚度，影响细胞的主要是基质的硬度及其交联程度，此厚度范围从几微米到60 μm不等，与接种细胞的水平尺寸有关。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0002-7063-2922(李珊)

关键词: 基质硬度, 基质厚度, 细胞铺展, 细胞增殖, 细胞分化, 干细胞, 国家自然科学基金

Abstract:

BACKGROUND: The mechanical properties of extracellular matrix can affect cell spreading, proliferation and differentiation. It is of extremely vital significance for tissue engineering and regenerative medicine.
OBJECTIVE: To summarize the progress of matrix mechanical properties influences on cell growth and to analyze if there is a relationship between matrix thickness and stiffness, in order to provide experimental methods for functional tissue construction in vitro.
METHODS: The author performed a data retrieval of PubMed and Bailianyun databases from 1988 to 2016 to search the articles addressing the influence of extracellular matrix mechanical properties on cell growth, and systematically reviewed the literatures.
RESULTS AND CONCLUSION: A total of 254 references were retrieved, and 43 articles were finally involved in the result analysis according to the inclusion and exclusion criteria. After summarizing and analyzing, it is found that the structure and function of cells adhering to the matrix are related to the extracellular environment. In vitro simulation experiments have shown that the increasing of matrix stiffness can change the cell morphology, increase the spreading area and proliferation rate, and can also influence the cell differentiation. When the matrix thickness is in a certain range, cells can sense the change of matrix thickness. When the matrix thickness is out of range, cells cannot perceive the matrix thickness. Matrix stiffness and cross-linking degree have certain effects on the cells, and the matrix thickness ranges from several microns to 60 microns, which is associated with the horizontal size of seeded cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Extracellular Matrix, Hardness, Cell Proliferation, Cell Differentiation, Tissue Engineering

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