中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (6): 889-895.doi: 10.3969/j.issn.2095-4344.0064

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

载锌功能化多壁碳纳米管/壳聚糖复合支架的制备与性能评价

杨  犇1，何东宁1，秦博恒1，张保平2
  

  1. 1山西医科大学口腔医学系，山西省太原市  030001；2中国科学院山西煤炭化学研究所，山西省太原市  030001
  • 收稿日期:2017-09-05 出版日期:2018-02-28 发布日期:2018-02-28
  • 通讯作者: 何东宁，副教授，山西医科大学口腔医学系，山西省太原市 030001
  • 作者简介:杨犇，男，1985年生，陕西省渭南市人，汉族，山西医科大学在读硕士，主要从事骨组织工程支架材料方面相关研究。
  • 基金资助:
    山西省卫生与计划生育委员会资助项目(2015112)

Preparation and performance properties of functionalized multi-walled carbon nanotubes/chitosan-zinc composite scaffolds

Yang Ben1, He Dong-ning1, Qin Bo-heng1, Zhang Bao-ping2
  

  1. 1Department of Stomatology, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; 2Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, Shanxi Province, China
  • Received:2017-09-05 Online:2018-02-28 Published:2018-02-28
  • Contact: He Dong-ning, Associate professor, Department of Stomatology, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • About author:Yang Ben, Studying for master’s degree, Department of Stomatology, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Supported by:
    the funded project of Shanxi Province Health and Family Planning Commission, No. 2015112

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文题释义：
功能化碳纳米管：利用强酸(如浓硝酸和浓硫酸)可对碳纳米管进行改性，在碳纳米管表面增加功能基团(如羧基、羟基等)可获得功能化碳纳米管。功能化碳纳米管显著改善了碳纳米管在水中的分散性及与聚合物反应的能力，此外，在聚合物基质中添加少量功能化碳纳米管，可改善材料的性能，促进材料与细胞间的相互作用，增加细胞代谢功能。
壳聚糖：是一种天然多糖，由β-(1→4)-2-乙酰氨基-d-葡萄糖和β-(1→4)-2-氨基-d-葡萄糖单元共聚而成，具有良好的生物相容性、生物可降解性、促进伤口愈合及抗微生物活性，然而，单纯使用壳聚糖作为骨组织工程支架时机械性能较差，在骨缺损区不能有效承重。
 
背景：单纯使用壳聚糖作为骨组织工程支架时机械性能较差，在骨缺损区不能有效承重，因此，许多学者尝试开发多壁碳纳米管/壳聚糖骨组织工程支架。
目的：构建载锌功能化多壁碳纳米管/壳聚糖复合支架，测试其力学性能、抗菌活性与细胞相容性。
方法：①采用“混酸法”制备功能化多壁碳纳米管；将壳聚糖、功能化多壁碳纳米管溶液(功能化多壁碳纳米管质量分数0.5%)与不同浓度的ZnSO4•7H2O(锌质量分数分别0.2%、1%、2%)混合，制备载锌功能化多壁碳纳米管/壳聚糖复合支架，检测纯壳聚糖支架及载锌功能化多壁碳纳米管/壳聚糖复合支架的表面硬度及拉伸强度；②采用抑菌圈实验检测纯壳聚糖支架及载锌功能化多壁碳纳米管/壳聚糖复合支架对金黄色葡萄球菌或大肠杆菌的抑制作用；③采用载锌功能化多壁碳纳米管/壳聚糖复合支架浸提液培养小鼠成骨细胞MC3T3-El，培养1，2，3 d后，采用MTT法检测细胞增殖，计算细胞相对增殖率，评价细胞毒性。
结果与结论：①3组载锌功能化多壁碳纳米管/壳聚糖复合支架的表面硬度、拉伸强度均高于纯壳聚糖支架   (P < 0.05)，3组载锌功能化多壁碳纳米管/壳聚糖复合支架间的表面硬度、拉伸强度比较差异无显著性意义；②3组载锌功能化多壁碳纳米管/壳聚糖复合支架对金黄色葡萄球菌和大肠杆菌的抗菌性能均高于纯壳聚糖支架(P < 0.05)，且抗菌性能随着复合支架中锌质量分数的增加而增强；③含锌质量分数0.2%、1%的载锌功能化多壁碳纳米管/壳聚糖复合支架无细胞毒性；④结果表明，含锌质量分数1%的载锌功能化多壁碳纳米管/壳聚糖复合支架具有良好的力学性能、抗菌活性及细胞相容性。

关键词: 功能化多壁碳纳米管, 锌, 复合支架, 力学性能, 生物材料, 骨生物材料, 壳聚糖, 抗菌性能

Abstract:

BACKGROUND: A pure chitosan (CS) scaffold has poor mechanical properties, and cannot play a load-bearing role in the bone defect region. Therefore, many scholars attempt to develop multi-walled carbon nanotube (MWCNT)/chitosan (MWCNT/CS) bone tissue scaffolds.
OBJECTIVE: To develop the functionalized MWCNT/CS composite scaffolds carrying zinc (f-MWCNT/CS-Zn) and to test its mechanical properties, antibacterial activity and biocompatibility.
METHODS: (1) F-MWCNT composite scaffolds were prepared by the acid mixture method, and f-MWCNT/CS-Zn composite scaffolds were prepared through mixing CS, f-MWCNT (0.5%) with different concentrations of ZnSO4•7H2O (the concentrations of Zn in the composite scaffolds were 0.2%, 1%, 2%, respectively). The surface hardness and tensile strength of the pure CS scaffold and the f-MWCNT/CS-Zn composite scaffolds were measured. (2) The inhibitory effect on Staphylococcus aureus or Escherichia coli of the pure CS scaffold and f-MWCNT/CS-Zn scaffolds was tested by inhibition zone test. (3) The osteoblasts MC3T3-El were cultured with the leaching solution of f-MWCNT/CS-Zn composite scaffolds. The cell proliferation was detected by MTT assay at 1, 2, 3 days after culture, then the relative proliferation rate was calculated and the cytotoxicity was evaluated.
RESULTS AND CONCLUSION: (1) The surface hardness and tensile strength of the f-MWCNT/CS-Zn composite scaffolds were higher than those of the pure CS scaffold (P < 0.05), and there was no significant difference in the surface hardness and tensile strength between the different zinc-containing composite scaffolds. (2) The antibacterial properties of the three f-MWCNT/CS-Zn composite scaffolds against Staphylococcus aureus and Escherichia coli were higher than those of the pure CS scaffold (P < 0.05), and the antibacterial activity was enhanced with the increase of zinc mass fraction. (3) The f-MWCNT/CS-0.2%Zn and f-MWCNT/CS-1%Zn composite scaffolds had no cytotoxicity. To conclude, the f-MWCNT/CS-1%Zn composite scaffold has good mechanical properties, antibacterial activity and biocompatibility.

Key words: Chitosan, Nanotubes, Carbon, Zinc, Tissue Engineering

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