中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3806-3810.doi: 10.3969/j.issn.1673-8225.2012.21.002

• 组织工程神经材料 tissue-engineered nerve materials • 上一篇    下一篇

外源性睫状神经营养因子干预缺损神经再生及运动功能的恢复*★

姜广擎1，张庭深2   

  1. 1沈阳市胸科医院，辽宁省沈阳市   110034；2沈阳医学院组胚教研室，辽宁省沈阳市  110034
  • 收稿日期:2011-11-02 修回日期:2012-03-31 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 张庭深，硕士，教授，沈阳医学院组胚教研室，辽宁省沈阳市 110034 syzhangts@yahoo.com.cn
  • 作者简介:姜广擎★，男，1967年生，汉族，吉林大学白求恩医学院毕业，硕士，主任医师，主要从事脊柱外科方面的工作。jgq31510@ sohu.com
  • 基金资助:

    沈阳市科委立项资助(1041048-1-02-07)。

Effects of exogenous ciliary neurotrophic factor on the regeneration and motor functional resumption of defected nerves 

Jiang Guang-qing1, Zhang Ting-shen2   

  1. 1Shenyang Chest Hospital, Shenyang  110034, Liaoning Province, China; 2Department of Histology and Embryology, Shenyang Medical College, Shenyang  110034, Liaoning Province, China
  • Received:2011-11-02 Revised:2012-03-31 Online:2012-05-20 Published:2012-05-20
  • Contact: Zhang Ting-shen, Master, Professor, Department of Histology and Embryology, Shenyang Medical College, Shenyang 110034, Liaoning Province, China syzhangts@yahoo.com.cn
  • About author:Jiang Guang-qing★, Master, Chief physician, Shenyang Chest Hospital, Shenyang 110034, Liaoning Province, China jgq31510@sohu.com
  • Supported by:

    the grant from the Science and Technology Committee of Shenyang City, No. 1041048-1-02-07*

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摘要：

背景：睫状神经营养因子作为生物活性因子家族的成员，因其生物活性的多效性而备受基础与临床研究的重视。
目的：探讨睫状神经营养因子对缺损神经再生及其运动功能恢复的影响及相关因素。
方法：40只大鼠建立高位神经缺损模型后随机数字表法均分成4组，3个实验组分别将不同质量浓度10，20，40 mg/L的外源性睫状神经营养因子液0.2 mL注入神经再生室内，对照组注入等量的生理盐水。两组干预后检测坐骨神经功能指数、神经肌肉动作电位、神经再生及其靶器官的形态学变化。
结果与结论：治疗后4周，各组坐骨神经功能指数差异无显著性意义(P > 0.05)。治疗后8，12周，各实验组的坐骨神经功能指数、运动神经传导速度、腓肠肌湿质量及截面积残存率均明显高于对照组(P < 0.05)，各组神经远段再生的有髓神经纤维数量、直径、截面积及髓鞘厚度均小于自体神经近段(P < 0.05)，其中以40 mg/L质量浓度指标变化最为显著(P < 0.05)。说明在修复缺损神经过程中，向由可降解生物膜形成的神经再生室内加入一定浓度的外源性睫状神经营养因子，对受损神经的结构再生与运动功能恢复有一定的促进作用。
关键词：睫状神经营养因子；坐骨神经功能指数；运动神经传导速度；坐骨神经；神经再生
doi:10.3969/j.issn.1673-8225.2012.21.002

关键词: 睫状神经营养因子, 坐骨神经功能指数, 运动神经传导速度, 坐骨神经, 神经再生

Abstract:

BACKGROUND: Ciliary neurotrophic factor (CNTF) is a member of bioactive factor family and has obtained more attention by basic and clinical research for its pleiotropic effects.
OBJECTIVE: to explore the effect and related factors about exogenous CNTF on the regeneration and motor functional resumption of defected nerves.
METHODS: Forty rats were used to prepare nerve defect models and then randomly divided into four groups. The different concentrations of 0.2 mL CNTF (10, 20, 40 mg/L) were injected to the position of nerve defects of rats in three experimental groups. The same amount of saline was injected into the rats of control group. After operation, sciatic functional index, muscle evoked action potential, and morphological index of nerve regeneration and target organ changes were detected.
RESULTS AND CONCLUSION: At 4 weeks after treatment, sciatic functional index had no changes in different groups (P > 0.05).   At 8 and 12 weeks, sciatic functional index, motor nerve conduction velocity, wet weight and cross-sectional area survival rate of gastrocnemius in the experimental groups were more than those in the control group (P < 0.05). The number, diameter, cross-sectional area of myelinated fiber and thickness of myelin sheath from regenerated nerve distal part were less than those from autologous nerve proximal part (P < 0.05), especially in the 40 mg/L group. The certain concentration of exogenous CNTF in nerve regenerative chamber wrapped with biodegradable membrane can promote nerve structural regeneration and motor function resumption.

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