中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 2003-2006.doi: 10.3969/j.issn.1673-8225.2012.11.024

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

青老年大鼠脑缺血模型的比较★

刘宗涛1，秦永生1，刘  江2，刘远新3，姜  涛4   

  1. 1新疆医科大学，新疆维吾尔自治区乌鲁木齐市 830054；新疆医科大学附属中医医院，2VIP病房，3神经内科，新疆维吾尔自治区乌鲁木齐市 830000；4新疆医科大学第一附属医院实验动物研究中心，新疆维吾尔自治区乌鲁木齐市  830054
  • 收稿日期:2011-09-20 修回日期:2011-10-13 出版日期:2012-03-11 发布日期:2012-03-11
  • 通讯作者: 刘江，主治医师，新疆医科大学附属中医医院VIP病房，新疆维吾尔自治区乌鲁木齐市 830000 liujiang1119@163.com
  • 作者简介:刘宗涛★，男，1983年生，安徽省太和县人，新疆医科大学在读硕士，主要从事脑血管病研究。

Young rat model versus old rat model for cerebral ischemia

Liu Zong-tao1, Qin Yong-sheng1, Liu Jiang2, Liu Yuan-xin3, Jiang Tao4   

  1. 1Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 2Ward of Very Important Person, 3Department of Neurology, Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous Region, Urumqi  830000, Xinjiang Uygur Autonomous Region, China; 4Experimental Animal Research Center, the First Teaching Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-09-20 Revised:2011-10-13 Online:2012-03-11 Published:2012-03-11
  • Contact: author: Liu Jiang, Attending physician, Ward of Very Important Person, Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830000, Xinjiang Uygur Autonomous Region, China liujiang1119@163.com
  • About author:Liu Zong-tao★, Studying for master’s degree, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China 1491983650@qq.com

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摘要：

背景：大鼠具有成本低，种系内纯合性好，脑血管解剖特性与人类相似等特点，是目前脑缺血研究最常用的实验动物。
目的：观察青老年大鼠大脑中动脉梗死后的行为学变化，分析年龄对脑缺血的影响。
方法：将青、老年SD大鼠随机分为青、老年假手术组，青、老年模型组(同侧颈总动脉永久结扎大脑中动脉线栓法制备大脑中动脉梗死模型)4组。
结果与结论：老年假手术组体质量低于青年假手术组(P < 0.05)，但高于老年模型组(P < 0.05)，老年模型组体质量低于青年模型组(P < 0.05)。术后第1，3，5，7天老年模型组改良神经功能损害程度评分高于青年模型组(P < 0.05)；与青年模型组及青、老年假手术组比较，术后第3，8，12周老年模型组逃避潜伏期明显延长，跨过平台所在位置的次数明显减少(P < 0.05)。表明在同等缺血打击下，老龄鼠脑缺血模型缺血损伤重、修复能力低，其神经功能恢复、学习和记忆能力明显逊于青年大鼠，提示增龄因素是研究脑缺血后神经损伤的重要影响因素之一。
关键词：大脑中动脉梗死；动物模型；改良神经损害程度评分；学习；记忆；组织构建实验造模 
doi:10.3969/j.issn.1673-8225.2012.11.024

关键词: 大脑中动脉梗死, 动物模型, 改良神经损害程度评分, 学习, 记忆, 组织构建实验造模 

Abstract:

BACKGROUND: At present, rats are the most commonly used experimental animals in cerebral ischemia research. Rats have many advantages, such as inexpensive, good intraspecific homozygosity and similar cerebrovascular anatomical characteristics with human beings.
OBJECTIVE: To explore the influence of aging to cerebral ischemia by observing the behavior changes in young and old rats with middle cerebral artery occlusion.
METHODS: Young and old Sprague Dawley rats were randomly divided into four groups: young sham-operation group, old sham-operation group, young model group and old model group. Middle cerebral artery occlusion model was established by permanent ligation of the ipsilateral common carotid artery using the intraluminal suture technique.
RESULTS AND CONCLUSION: The body mass index of the old sham-operation group was significantly lower than that of the young sham-operation group (P < 0.05), but higher than that of the old model group (P < 0.05). The body mass index of the old model group was lower than that of the young model group (P < 0.05). The modified neurological severity score of the old model group was significantly higher than that of the young model group on the 1st, 3rd, 5th and 7th days after operation (P < 0.05). The escape latency of the old model group was significantly longer than those of the other groups at the 3rd, 8th and 12th weeks after operation. The number of crossing platform location in the old model group was significantly less than those in the other groups   (P < 0.05, respectively). These findings demonstrated that compared with the young rat cerebral ischemia model with the same ischemia state, the old rat cerebral ischemia model showed more severe ischemia damage, lower repair capacity, and the neural functional recovery, spatial learning and memory ability was obvious inferior to that of the young rat model. It indicates that aging is one of the important factors that influence the neural injury after cerebral ischemia.

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