中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (10): 1761-1764.doi: 10.3969/j.issn.1673-8225.2012.10.012

• 胚胎干细胞 embryonic stem cells • 上一篇    下一篇

重组人促红细胞生成素干预人源羊水干细胞向心肌前体细胞的分化*★

刘  燕1，李法琦1，张  彬2，骆建平3   

  1. 重庆医科大学附属第一医院，1老年科，3临床产前诊断中心，重庆市  400016；2重钢总医院心内科，重庆市  400080
  • 收稿日期:2012-01-09 修回日期:2012-02-02 出版日期:2012-03-04 发布日期:2012-03-04
  • 通讯作者: 李法琦，博士，教授，主任医师，硕士生导师，重庆医科大学附属第一医院老年病科，重庆市 400016 faqili_2006@yahoo.com
  • 作者简介:刘燕★，女，1985年生，重庆市人，汉族，重庆医科大学在读硕士，主要从事老年心血管疾病基础与临床研究。 liuyan2009110177@163.com
  • 基金资助:

    重庆市卫生局医学科研项目(2009-2-367)，课题名称：促红细胞生成素调节经典Wnt信号途径促进羊水来源诱导多能干细胞心肌定向分化的研究

Effect of recombinant human erythropoietin on differentiation of myocardial precusor cells from amniotic fluid stem cells of pregnancy women

Liu Yan1, Li Fa-Qi1, Zhang Bin2, Luo Jian-Ping3   

  1. 1Department of Geriatrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; 2Department of Cardiology, the General Hospital of Chongqing Iron and Steel Group, Chongqing 400080, China; 3Center of Prenatal Diagnosis, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
     
  • Received:2012-01-09 Revised:2012-02-02 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Li Fa-Qi, M.D., Professor, Chief physician, Master’s supervisor, Department of Geriatrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China faqili_2006@yahoo.com
  • About author:Liu Yan★, Studying for master’s degree, Department of Geriatrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China liuyan2009110177@163.com
  • Supported by:

    Medical Scientific Foundation of Chongqing Health Bureau, No. 2009-2-367*

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摘要：

背景：研究发现人源羊水干细胞可向心肌前体细胞分化。
目的：观察不同浓度重组人促红细胞生成素对人源羊水干细胞向心肌前体细胞分化的影响。
方法：用含0，1.0，5.0，10.0，20.0 U/mL重组人促红细胞生成素的诱导培养基诱导鉴定后的人源羊水干细胞向心肌前体细胞分化，诱导24 h后换为完全培养基继续培养。
结果与结论：诱导分化14 d，倒置显微镜下可见细胞由长梭形逐渐变短增宽，相邻的细胞间有融合现象，似类肌管样结构，以5.0 U/mL重组人促红细胞生成素的诱导分化率最高；RT-PCR检测显示重组人促红细胞生成素可促进细胞Nkx-2.5和GATA-4 mRNA的表达，以5.0 U/mL重组人促红细胞生成素的作用最强。说明外源性重组人促红细胞生成素能剂量依赖性促进人源羊水干细胞向心肌前体细胞分化。

关键词: 重组人促红细胞生成素, 人源羊水干细胞, 心肌前体细胞, Nkx-2.5, GATA-4, 干细胞

Abstract:

BACKGROUND: Studies have shown that amniotic fluid stem cells of pregnancy women can differentiate into myocardial precursor cells.
OBJECTIVE: To study the effect of recombinant human erythropoietin (rhEPO) at different concentrations on differentiation of myocardial precusor cells from amniotic fluid stem cells of pregnancy women.
METHODS: The differentiation of myocardial precursor cells from amniotic fluid stem cells of pregnancy women was induced by adding conditional culture medium containing 0, 1.0, 5.0, 10.0, 20.0 U/mL rhEPO. After 24 hours, conditional culture medium was replaced by complete medium.
RESULTS AND CONCLUSION: During 14 days of induced differentiation, under the inverted microscope, cells gradually shortened and widened from original shuttle appearance. Adjacent cells fused together, similar to myotube-like structure. The differentiation rate of myocardial precursor cells was highest after 5.0 U/mL rhEPO addition. RT-PCR results showed that rhEPO particularly at the concentration of 5.0 U/mL could promote Nkx-2.5 and GATA-4 mRNA expression. These findings suggest that exogenous rhEPO can promote the differentiation of myocardial precursor cells from amniotic fluid stem cells of pregnancy women dose-dependently.

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