中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (9): 1521-1525.doi: 10.3969/j.issn.1673-8225.2012.09.001

• 数字化骨科 digital orthopedics •    下一篇

静水压下芹菜素对体外培养人髓核细胞凋亡率及基质代谢的影响*☆

柳根哲，陈  江，徐  林，李春根，孙  旗，乔卫平，朱志强   

  1. 北京中医药大学东直门医院骨科，北京市  100700
  • 收稿日期:2011-11-21 修回日期:2011-12-29 出版日期:2012-02-26 发布日期:2012-02-26
  • 作者简介:柳根哲☆，男，1964年生，黑龙江省牡丹江市人，朝鲜族，2000年日本国立富山医科药科大学毕业，博士，副教授，硕士研究生导师，主要从事脊柱外科及脊柱微创技术、椎间盘的生物力学、退变与再生的基础研究。liu_genzhe2000@yahoo.com.cn
  • 基金资助:

    课题接受了国家自然科学基金资助(30873274)。

Effect of apigenin on apoptotic rate and matrix metabolism of human nucleus pulposus cells cultured in vitro under hydrostatic pressure  

Liu Gen-zhe, Chen Jiang, Xu Lin, Li Chun-gen, Sun Qi, Qiao Wei-ping, Zhu Zhi-qiang   

  1. Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing  100700, China
  • Received:2011-11-21 Revised:2011-12-29 Online:2012-02-26 Published:2012-02-26
  • About author:Liu Gen-zhe☆, Doctor, Associate professor, Master’s supervisor, Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China liu_genzhe2000@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30873274*

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摘要：

背景：生物力学环境的异常及一氧化氮过度表达是椎间盘退变进程中重要的影响因素。
目的：探讨静水压下芹菜素对体外单层培养的人髓核细胞凋亡率及基质代谢的影响。
方法：在静水压加载系统中对体外单层培养的人髓核细胞(传4代)施以0.1~3.0 MPa的静压，加压2 h，同时使用芹菜素和一氧化氮供体对髓核细胞进行干预，分析不同静水压下芹菜素对髓核细胞凋亡率、基质金属蛋白酶及基质表达的影响。
结果与结论：在0.3 MPa静水压下，芹菜素能改善由于高浓度一氧化氮引起的髓核细胞功能抑制状态，降低基质金属蛋白酶3过表达，恢复基质金属蛋白酶3与其抑制因子1之间的平衡；而在3 MPa静水压下，芹菜素能降低一氧化氮引起的髓核细胞高凋亡，并在一定程度上缓解基质金属蛋白酶3与其抑制因子1之间的不平衡。说明芹菜素能够对髓核细胞的功能状态、凋亡率，对基质金属蛋白酶3及其抑制因子1平衡产生影响，而且这种影响在不同的静水压环境下有所不同。
关键词：静水压；芹菜素；髓核细胞；凋亡率；基质金属蛋白酶
doi:10.3969/j.issn.1673-8225.2012.09.001

关键词: 静水压, 芹菜素, 髓核细胞, 凋亡率, 基质金属蛋白酶

Abstract:

BACKGROUND: Abnormal biomechanical environment and over-expression nitric oxide are the two important factors in the process of intervertebral disc degeneration.
OBJECTIVE: To discuss the effect of apigenin on the apoptotic rate and matrix metabolism of monolayer cultured human nucleus pulposus cells in vitro under different hydrostatic pressures.
METHODS: In the hydrostatic pressure loading system, 0.1-3 MPa pressure was imposed on the human nucleus pulposus cells (passage 4) in monolayer culture in vitro for 2 hours, while using apigenin and nitric oxide donor to intervene on nucleus pulposus cells, and analyzing the effect of apigenin on the apoptotic rate, matrix metalloproteinase and matrix metabolism of human nucleus pulposus cells under hydrostatic pressure.
RESULTS AND CONCLUSION: Under 0.3 MPa hydrostatic pressure, apigenin could reduce the degradation of extracellular matrix by regulating the balance between matrix metallo proteinases-3 (MMP-3) and human tissue inhibitor of metal protease-1 (TIMP-1), and improving nucleus pulposus cells inhibition status due to the high concentration of nitric oxide. Under 3 MPa hydrostatic pressure, apigenin had a role in reducing the apoptotic rate of nucleus pulposus cells and could alleviate the unbalance between the MMP-3 and TIMP-1. The results showed that apigenin has an influence on the function status and apoptotic rate of nucleus pulposus cells, the balance between MMP-3 and TIMP-1, and the effects are different under different hydrostatic pressure environments.

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