中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (3): 455-458.doi: 10.3969/j.issn.1673-8225.2012.03.016

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

丝素蛋白/壳聚糖复合支架材料的细胞相容性**★

佘荣峰，邓  江，黄文良，董志军，刘  彬   

  1. 遵义医学院第三附属医院骨科，贵州省遵义市 
    563002
  • 收稿日期:2011-07-20 修回日期:2011-08-24 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 邓江，主任医师，研究生导师，遵义医学院第三附属医院骨科，贵州省遵义市 563002 DJ30666@ 126.com
  • 作者简介:佘荣峰★，男，1984年生，贵州省遵义市人，汉族，遵义医学院在读硕士，医师，主要从事骨与关节创伤方面的研究。 396008507@ qq.com
  • 基金资助:

    贵州省科学技术基金项目(黔科合J字[2009]2172号)，课题名称：SF-CS复合诱导骨髓间充质干细胞修复兔膝关节软骨缺损的研究；遵义市科学技术局资助项目(遵市科合社字[2010]015号)，课题名称：RGD表面修饰SF/CS/n-HA材料复合携带目的基因的BMSCs成骨性能研究。

Cellular biocompatibility of silk fibroin/chitosan composite scaffolds 

She Rong-feng, Deng Jiang, Huang Wen-liang, Dong Zhi-jun, Liu Bin   

  1. Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Received:2011-07-20 Revised:2011-08-24 Online:2012-01-15 Published:2012-01-15
  • Contact: Deng Jiang, Chief physician, Master’s supervisor, Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China DJ30666@126.com
  • About author:She Rong-feng★, Studying for master’s degree, Physician, Department of Orthopaedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • Supported by:

    the Science and Technology Foundation of Guizhou Province, No. [2009]2172*; a grant from the Science and Technology Bureau of Zuiyi City, No. [2010]15*

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摘要：

背景：大量研究表明丝素蛋白、壳聚糖为天然高分子材料，具有良好的细胞生物相容性。
目的：探讨丝素蛋白/壳聚糖复合支架材料与诱导的兔骨髓间充质干细胞的生物相容性。
方法：将兔骨髓间充质干细胞分离培养、诱导后，与丝素蛋白/壳聚糖三维支架材料体外共培养，以材料的细胞毒性、细胞增殖活力、材料细胞黏附率及扫描电镜等检测评价材料的细胞相容性。
结果与结论：经诱导后的骨髓间充质干细胞在支架材料上黏附、生长良好，保持正常的分裂增殖速度；随时间的增加，细胞黏附率增加，材料组较对照组黏附率强，差异有显著性意义(P < 0.05)。扫描电镜观察发现细胞接种48 h后细胞生长良好，与支架黏附紧密，增殖分裂活跃。说明丝素蛋白/壳聚糖三维支架材料具有良好的细胞相容性。

关键词: 丝素蛋白, 壳聚糖, 细胞相容性v支架v骨髓间充质干细胞

Abstract:

BACKGROUND: Many studies show that silk fibroin (SF) and chitosan (CS) are natural polymer materials that have good biocompatibility. 
OBJECTIVE: To discuss the biocompatibility of induced rabbit bone marrow mesenchymal stem cells (BMSCs) with SF/CS composite scaffolds.
METHODS: Rabbit BMSCs were isolated, cultured and induced, and then cocultured with three-dimensional SF/CS scaffolds   in vitro. Cell toxicity of scaffolds, cell viability, cell adhesion rate were determined to evaluate cellular biocompatibility of the materials as well as scanning electron microscope (SEM) observation.
RESULTS AND CONCLUSION: After being induced, the rabbit BMSCs adhered and grew well on the scaffolds to keep normal division and proliferation. As time went, the cell adhesion rate increased and the scaffolds group had a stronger adhesion rate than the control group, and the difference had statistic meaning (P < 0.05). The SEM observation found that the cells grew well after 48 hours, which were closely adhered to scaffolds as well as divided and proliferated actively. The above findings indicate that the three-dimensional SF/CS scaffolds have a better cellular biocompatibility.

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