中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9397-9401.doi: 10.3969/j.issn.1673-8225.2011.50.022

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

外源性酸性成纤维细胞生长因子干预肠缺血-再灌注损伤中p38MAPK和成纤维细胞生长因子受体2的表达

翁立新1，李秀霞2，付小兵3   

  1. 1内蒙古医学院病理教研室，内蒙古自治区呼和浩特市  010059
    2内蒙古医学院附属医院病理科，内蒙古自治区呼和浩特市  010059
    3解放军总医院创伤修复研究室，北京市  100853 
  • 收稿日期:2011-06-19 修回日期:2011-09-26 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 李秀霞，教授，硕士生导师，内蒙古医学院附属医院病理科，内蒙古自治区呼和浩特市 010059 nmglxx@sina.com
  • 作者简介:翁立新★，女，1966年生，内蒙古自治区呼和浩特市人，汉族，硕士，副教授，主要从事创伤修复的研究。 wenglixin2007@yahoo.cn
  • 基金资助:

    国家自然科学基金重点项目(30230370)；内蒙古教育厅一般研究课题资助(NJZY07093)。

Effects of exogenous acidic fibroblast growth factor on the expression of p38MAPK and fibroblast growth-factor receptor 2 in rats with intestinal ischemia-reperfusion injury

Weng Li-xin1, Li Xiu-xia2, Fu Xiao-bing3   

  1. 1Department of Pathology, Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China
    2Department of Pathology, Affiliated Hospital of Inner Mongolia Medical College, Hohhot  010059, Inner Mongolia Autonomous Region, China
    3Laboratory of Wound Repair, General Hospital of Chinese PLA, Beijing 100853, China
  • Received:2011-06-19 Revised:2011-09-26 Online:2011-12-10 Published:2011-12-10
  • Contact: Li Xiu-xia, Professor, Master’s supervisor, Department of Pathology, Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China nmglxx@sina.com
  • About author:Weng Li-xin★, Master, Associate professor, Department of Pathology, Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China wenglixin2007@yahoo.cn
  • Supported by:

    the Key Program of the National Natural Science Foundation of China, No. 30230370*; the General Research Program of Education Bureau of Inner Mongolia Autonomous Region, No. NJZY07093*

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被引次数

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摘要：

背景：酸性成纤维细胞生长因子可以促进多种创面愈合，在内脏损伤修复中起重要促进作用。
目的：观察外源性酸性成纤维细胞生长因子干预肠缺血-再灌注损伤大鼠后p38MAPK和成纤维细胞生长因子受体2的表达。
方法：以大鼠肠系膜上动脉夹闭45 min造成肠缺血-再灌注损伤模型，于再灌注即刻应用改构体酸性成纤维细胞生长因子进行干预。分别于再灌注2，6，12，24 h取大鼠小肠组织标本，用于实验。
结果与结论：在正常大鼠，成纤维细胞生长因子受体2主要分布在小肠绒毛上皮细胞的肠腔侧、侧壁和小肠隐窝朝向隐窝腔的一侧细胞膜上。缺血-再灌注初期，p38MAPK蛋白及成纤维细胞生长因子受体2蛋白和mRNA的表达未发生明显变化，随着再灌注时间的延长其逐渐增强，并于再灌注后6~12 h达高峰。经酸性成纤维细胞生长因子治疗后，大鼠小肠组织p38MAPK蛋白及成纤维细胞生长因子受体2蛋白和mRNA的表达进一步增强，小肠黏膜损伤程度减轻。说明酸性成纤维细胞生长因子可通过上调成纤维细胞生长因子受体2和p38MAPK的表达促进肠缺血-再灌注损伤的修复。

关键词: 酸性成纤维细胞生长因子, 受体, p38MAPK, 缺血-再灌注损伤, 小肠

Abstract:

BACKGROUND: Different types of wound healing can be promoted by acidic fibroblast growth factors. It plays an important role in the repair of visceral injury.
OBJECTIVE: To explore the effect of exogenous acidic fibroblast growth factor on the expression of p38MAPK and fibroblast growth-factor receptor 2 in rats with intestinal ischemia-reperfusion injury.
METHODS: Rat superior mesenteric artery was microsurgical clipping for 45 minutes to construct intestinal ischemia-reperfusion injury model. Rats were treated with modified acidic fibroblast growth factor immediately after reperfusion. Rat small intestines samples were collected at the 2nd, 6th, 12th and 24th hours after reperfusion to examine.
RESULTS AND CONCLUSION: Fibroblast growth-factor receptors 2 were mainly distributed in epithelial cells of small intestinal villi at intestinal cavity, side wall and on the cell membrane of crypts toward cavity. There was no significant change in the expression of fibroblast growth-factor receptor 2 and p38MAPK in protein and mRNA level at the primary stage after reperfusion. Then the expression of fibroblast growth-factor receptor 2 and p38MAPK increased gradually along with the extension of time, and peaked at the 6th to 12th hours after reperfusion. The protein and mRNA expression of fibroblast growth-factor receptor 2 and p38MAPK in rat small intestines was further enhanced after treated with acidic fibroblast growth factor. These findings demonstrate that acidic fibroblast growth factor can promote the repair of intestinal ischemia-reperfusion injury by up-regulating the expression of fibroblast growth-factor receptor 2 and p38MAPK.

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