中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9393-9396.doi: 10.3969/j.issn.1673-8225.2011.50.021

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

重组人表皮生长因子联合前列地尔作用于糖尿病溃疡创面

陈杰翔1,安宏元2   

  1. 1泸州医学院附属医院泌尿外科,四川省泸州市 646000
    2吉林省柳河县人民医院内分泌科,吉林省通化市 135300
  • 收稿日期:2011-05-28 修回日期:2011-06-07 出版日期:2011-12-10 发布日期:2011-12-10
  • 作者简介:陈杰翔,男,1976年生,四川省泸州市人,汉族,2001年泸州医学院毕业,主治医师,主要从事泌尿外科方面的研究。 fgh66666@sina.com

Recombinant human epidermal growth factor combined with alprostadil for treatment of diabetic ulcers

Chen Jie-xiang1, An Hong-yuan2   

  1. 1Department of Urology, Affiliated Hospital of Luzhou Medical College, Luzhou  646000, Sichuan Province, China
    2Department of Endocrinology, People’s Hospital of Liuhe County, Tonghua  135300, Jilin Province, China
  • Received:2011-05-28 Revised:2011-06-07 Online:2011-12-10 Published:2011-12-10
  • About author:Chen Jie-xiang, Attending physician, Department of Urology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China fgh66666@sina.com

摘要:

背景:人内源性表皮生长因子的缺乏以及血流动力学的改变会导致创面不愈合的发生。
目的:观察重组人表皮生长因子联合前列地尔作用于糖尿病溃疡动物模型创面的疗效。
方法:Wistar大鼠40只建立糖尿病溃疡动物模型,随机等分为模型组、重组人表皮生长因子组、前列地尔组和重组人表皮生长因子+前列地尔组,分别予以1%碘伏清创、重组人表皮生长因子凝胶外敷、前列地尔静脉滴注、重组人表皮生长因子凝胶外敷和前列地尔静脉滴注联合治疗。
结果与结论:干预后3,7,10,14 d观察发现,相比于模型组,重组人表皮生长因子和/或前列地尔治疗后,糖尿病皮肤溃疡大鼠溃疡面积减小、愈合时间缩短、创面动态愈合率上升(P < 0.01),且两者联合治疗的效果优于重组人表皮生长因子和前列地尔单独治疗(P < 0.01),而重组人表皮生长因子或前列地尔单独治疗的效果接近,提示重组人表皮生长因子与前列地尔联合使用比单纯使用前列地尔或单纯使用重组人表皮生长因子更能显著促进糖尿病溃疡创面的愈合。

关键词: 重组人表皮生长因子, 糖尿病, 皮肤溃疡, 模型, 前列地尔

Abstract:

BACKGROUND: Previous studies demonstrated that the lack of endogenous recombinant human epidermal growth factor (rhEGF) and the change of hemodynamic parameters will lead to nonunion.
OBJECTIVE: To observe the effect of rhEGF combined with alprostadil on diabetic ulcers.
METHODS: Forty Wistar rat models of diabetic ulcers were established and randomly divided into four groups: control (1% povidone iodine for debridement), rhEGF (rhEGF gel for spreading), alprostadil (intravenous administration of alprostadil) and rhEGF + alprostadil (rhEGF gel for spreading combined with intravenous administration of alprostadil).
RESULTS AND CONCLUSION: At 3, 7, 10 and 14 days after intervention, the area of diabetic ulcers was diminished, healing time was shortened, and dynamic healing rate of wound surface was increased in the alprostadil , rhEGF and alprostadil + rhEGF groups (P < 0.01). The therapeutic effects were superior in the alprostadil + rhEGF group to those in the rhEGF group and alprostadil group (P < 0.01). The therapeutic effects were similar between rhEGF group and alprostadil group. These findings suggest that rhEGF combined with alprostadil better promotes the healing of diabetic ulcers than simple use of rhEGF or alprostadil.

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