中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8702-8705.doi: 10.3969/j.issn.1673-8225.2011.46.036

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

补肾健脾方干预去势大鼠骨骼肌caspase-3和caspase-8的表达

李  颖1，白  波2, 3，黄宏兴4，吴伙燕1，张姝江3，王广伟4   

  1. 1广东省中西医结合医院，广东省佛山市  528200
    2广州医学院第一附属医院，广东省广州市 510120
    3广东省骨科矫形技术及植入材料重点实验室，广东省广州市 510120
    4广州中医药大学，广东省广州市 510405
  • 收稿日期:2011-05-21 修回日期:2011-05-30 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 李颖☆，男，主治医师，博士，主要从事骨质疏松的防治研究。 leewinly@163.com
  • 作者简介:李颖☆，男，主治医师，博士，主要从事骨质疏松的防治研究。 leewinly@163.com
  • 基金资助:

    中国博士后基金(20100480737)；广东省科技计划项目(2010B030700076)；广州市中医药科技项目(201122231001)。

Effects of the prescription for tonifying kidney and strengthening spleen on caspase-3 and caspase-8 expression in skeletal muscle of ovariectomized rats

Li Ying1 , Bai Bo2, 3, Huang Hong-xing4, Wu Huo-yan1, Zhang Shu-jiang3, Wang Guang-wei4   

  1. 1Department of Orthopedics, Guangdong Hospital of Integrated Traditional and Western Medicine, Foshan  528200 Guangdong Province, China
    2Department of Orthopedics, First Affiliated Hospital of Guangzhou Medical University, Guangzhou  510120, Guangdong Province, China
    3Guangdong Key Laboratory of Orthopedic Technology and Implant Materials, Guangzhou  510120, Guangdong Province, China
    4Guangzhou University of Traditional Chinese Medicine, Guangzhou  510405, Guangdong Province, China
  • Received:2011-05-21 Revised:2011-05-30 Online:2011-11-12 Published:2011-11-12
  • Contact: Li Ying☆, Doctor, Attending physician, Department of Orthopedics, Guangdong Hospital of Integrated Traditional and Western Medicine, Foshan 528200 Guangdong Province, China leewinly@163.com
  • About author:Li Ying☆, Doctor, Attending physician, Department of Orthopedics, Guangdong Hospital of Integrated Traditional and Western Medicine, Foshan 528200 Guangdong Province, China leewinly@163.com
  • Supported by:

    the Science Research Foundation for Post-doctoral Research of China, No. 20100480737*; the Science and Technology Development Project of Guangdong Province, No. 2010B030700076*; the Traditional Chinese Medicine Project of Guangzhou City, No. 201122231001*

PDF

340

被引次数

9

摘要：

背景：骨骼肌与骨质疏松症关系密切，导致其衰退的主要原因是细胞的凋亡，这种凋亡可能是Caspase家族蛋白所介导的。
目的：探讨补肾健脾方对去势大鼠骨骼肌caspase-3和 caspase-8调控作用。
方法：SD大鼠48只等随机分为对照组、模型组、中药组、西药组，后3组大鼠摘除双侧卵巢建立骨质疏松模型，对照组仅切除周围少量脂肪组织。造模2周后，中药组给予补肾健脾方(2.979 g/kg)灌胃，西药组给予戊酸雌二醇片(0.104 mg/kg)灌胃，模型组和对照组给予蒸馏水灌胃，1次/d。
结果与结论：①干预12周后，双能X线骨密度仪测量发现，相比于对照组，模型组大鼠左侧股骨的骨密度值和骨矿含量均明显降低(P < 0.01)；而中药组和西药组的骨密度值均高于模型组(P < 0.05)，且2组骨密度值和骨矿含量接近(P > 0.05)。②酶标免疫分析显示，与对照组相比，模型组骨骼肌中Caspase-3和 Caspase-8表达水平明显上升(P < 0.05)。中药能显著降低大鼠骨骼肌中Caspase-3的表达水平(P < 0.05)，而西药能显著减低Caspase-8表达水平(P < 0.05)。③说明补肾健脾方对卵巢切除所致的骨质疏松症有明显的治疗作用，能明显提高骨密度，而且能显著减低Caspase-3的水平，但不能显著减低Caspase-8水平，显示补肾健脾方不是通过死亡受体途径去抑制细胞凋亡的。

关键词: 补肾健脾方, 去势大鼠, 半胱氨酸天冬氨酸特异性蛋白酶, 骨骼肌, 骨质疏松

Abstract:

BACKGROUND: Studies have shown that there is a close relationship between skeletal muscle and osteoporosis. Apoptosis is a main reason of skeletal muscle aging, which may be mediated by the proteins of caspase family.
OBJECTIVE: To study the mechanism of the prescription for tonifying kidney and strengthening spleen on preventing and treating osteoporosis and observe the regulation of the prescription on caspase-3 and caspase-8 expression in skeletal muscle of ovariectomized rats.
METHODS: Forty-eight female non-pregnant SD rats (6 months) were randomly and evenly divided into four groups: control, ovariectomized, estrogen and traditional Chinese herb. After establishing model, the rats of traditional Chinese herb group were intragastrically administered with the prescription for tonifying kidney and strengthening spleen (2.979 g/kg). The rats of estrogen group received an intragastric administration of estradiol valerate (0.104 mg/kg). The rats of the other groups were intragastrically given distilled water, once a day.
RESULTS AND CONCLUSION: Dual energy X-ray absorptiometry results showed that compared with control group, bone mineral density (BMD) and bone mineral content (BMC) in rat left femur were significantly lower in the model group (P < 0.01). The BMD was significantly greater in the estrogen and traditional Chinese herb groups than in the model group (P < 0.05) and the BMD and BMC were similar between the estrogen and traditional Chinese herb groups (P > 0.05). Enzyme linked immunosorbent assay results showed that compared with the control group, caspase-3 and caspase-8 expression in the skeletal muscle was significantly increased in the model group (P < 0.05); caspase-3 expression was significantly decreased in the traditional Chinese herb group (P < 0.05), and caspase-8 expression was significantly decreased in the estrogen group (P < 0.05). These findings suggest that the prescription for tonifying kidney and strengthening spleen exhibits obvious therapeutic effects on ovariectomy-induced osteoporosis, can increase bone mineral density and obviously decrease caspase-3 expression, but it can not markedly decrease caspase-8 expression. This indicates that the prescription does not inhibit apoptosis by death receptor signal pathway.

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