中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8698-8701.doi: 10.3969/j.issn.1673-8225.2011.46.035

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

葛根素联合雌二醇对去卵巢大鼠骨质疏松症的治疗作用

李  海，王金花，黄海玲，黎  飚   

  1. 右江民族医学院组织胚胎学教研室，广西壮族自治区百色市  533000
  • 收稿日期:2011-04-19 修回日期:2011-05-08 出版日期:2011-11-12 发布日期:2011-11-12
  • 作者简介:李海，男，1961年生，广西壮族自治区博白县人，汉族，1986年广西医科大学毕业，副教授，主要从事老年性骨质疏松症中西医结合治疗研究。
  • 基金资助:

    广西自然科学基金(2010GXNSFA013268)，右江民族医学院科研课题(yk2009002)，课题名称均为葛根素联合雌二醇对去卵巢SD大鼠骨组织和血钙磷影响。

Puerarin combined with estradiol for treatment of osteoporosis in ovariectomized rats

Li Hai, Wang Jin-hua, Huang Hai-ling, Li Biao   

  1. Department of Histology and Embryology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-19 Revised:2011-05-08 Online:2011-11-12 Published:2011-11-12
  • About author:Li Hai, Associate professor, Department of Histology and Embryology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China lihaiying702@163.com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2010GXNSFA013268*; a Scientific Research Project of Youjiang Medical College for Nationalities, No. yk2009002*

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531

被引次数

7

摘要：

背景：雌激素治疗绝经后骨质疏松症产生的不良反应较大。
目的：观察小剂量葛根素联合雌二醇对去卵巢大鼠骨组织的影响。
方法：120只健康雌性大白鼠等分成只切除卵巢旁的一小块脂肪垫的假手术组和切除双侧卵巢的去卵巢模型组、葛根素组、雌二醇组及葛根素+雌二醇组。1周后葛根素组、雌二醇组和雌二醇+葛根素组分别皮下注射葛根素(50 mg/kg，1次/d)、雌二醇(200 μg/kg，2次/周)和雌二醇(100 μg/kg，2次/周)+葛根素(25 mg/kg，1次/d)。
结果与结论：去卵巢模型组大鼠骨组织稀疏，骨钙、磷水平和骨密度明显低于假手术组 (P < 0.01)，而经雌二醇和/或葛根素治疗后大鼠骨组织形态明显改善、骨钙、磷水平和骨密度明显升高。其中小剂量的葛根素联合雌二醇治疗与较大剂量的葛根素或雌二醇治疗效果接近。提示较小剂量的雌二醇与葛根素联合治疗也可对去卵巢大鼠骨质疏松症产生良好的治疗效果。

关键词: 葛根素, 雌二醇, 去卵巢, 大鼠, 骨质疏松症, 组织工程

Abstract:

BACKGROUND: Estradiol produces great adverse effects in treatment of postmenopausal osteoporosis.
OBJECTIVE: To investigate the effects of small dose of estradiol combined with estradiol on bone tissue of ovariectomized rats.
METHODS: A total of 120 healthy female rats were randomly and evenly divided into five groups: sham-operated (only resection of a small block of para-ovarian fat pad), ovariectomized (without drugs), puerarin, estradiol, and puerarin + estradiol. One week later, rats from the puerarin, estradiol and puerarin + estradiol groups were subcutaneously injected with puerarin (50 mg/kg, once a day), estradiol (200 μg/kg, twice a week), estradiol puerarin (25 mg/kg, once a day) + (100 μg/kg, twice a week), respectively.
RESULTS AND CONCLUSION: In the ovariectomized group, rat bone tissue was sparse, bone calcium and phosphorus levels as well as bone mineral density were significantly lower compared with sham-operated group (P < 0.01). After estradiol and/or puerain treatment, rat bone tissue morphology was obviously improved, and bone calcium and phosphorus levels and bone mineral density were significantly increased. The above-mentioned indices were similar after small dose of puerarin combined with estradiol treatment and high dose of puerarin combined with estradiol treatment. These findings suggest that small dose of estradiol combined with estradiol treatment yields satisfactory curative effects in treatment of osteoporosis in ovariectomized rats.

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