中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8681-8684.doi: 10.3969/j.issn.1673-8225.2011.46.031

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

仓鼠深Ⅱ度烧伤创面愈合过程中糜蛋白酶的表达

董祥林1，耿中利2，高伟成1，赵  阳1，秦  涛1，乔  星1，马  娟1，马少林1   

  1. 1新疆医科大学第一附属医院烧伤整形科，新疆维吾尔自治区乌鲁木齐市  830054
    2新疆医科大学附属肿瘤医院乳腺头颈外科，新疆维吾尔自治区乌鲁木齐市  830011
  • 收稿日期:2011-04-19 修回日期:2011-08-16 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 马少林，硕士，主任医师，新疆医科大学第一附属医院烧伤整形科，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:董祥林★，男，1974年生，安徽省泗县人，汉族，2005年新疆医科大学毕业，硕士，副主任医师，主要从事创面愈合方面的研究。
  • 基金资助:

    新疆医科大学第一附属医院青年科学基金(2010QN11)，课题名称：仓鼠深Ⅱ度烫伤模型的建立以及应用。

Chymase expression in deep partial-thickness burn wounds after burn injury in hamsters

Dong Xiang-lin1, Geng Zhong-li2, Gao Wei-cheng1, Zhao Yang1, Qin Tao1, Qiao Xing1, Ma Juan1, Ma Shao-lin1   

  1. 1Department of Burns and Plastic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
    2Department of Breast, Head and Neck Surgical Oncology, Affiliated Tumour Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • Received:2011-04-19 Revised:2011-08-16 Online:2011-11-12 Published:2011-11-12
  • Contact: Ma Shao-lin, Master, Chief physician, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China mashaolin9@163.com
  • About author:Dong Xiang-lin★, Master, Associate chief physician, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    Youth Science Foundation of the First Affiliated Hospital of Xinjiang Medical University, No. 2010QN11*

PDF

365

被引次数

8

摘要：

背景：肥大细胞糜蛋白酶在烧伤创面表达的研究较少。
目的：观察肥大细胞糜蛋白酶是否存在于烫伤创面以及深Ⅱ度烫伤后不同时间点的表达变化。
方法：应用75 ℃的水接触仓鼠背部皮肤12 s制备深Ⅱ度烫伤创面(病理组织学证实)。分别于烫伤前及烫伤后1，3，7，14 d取烫伤组织进行试验。
结果与结论：实时定量RT-PCR及放射免疫检测结果显示烫伤后创面组织中糜蛋白酶mRNA表达量和活性均有所增高，在烫伤后第3天最高。表明肥大细胞糜蛋白酶在烧伤创面有所表达，并可能参与了烧伤损伤愈合过程。

关键词: 深Ⅱ度烧伤, 糜蛋白酶, 肥大细胞, 创面, 愈合, 仓鼠

Abstract:

BACKGROUND: Little is known about mast cell chymase in burn injury in hamsters.
OBJECTIVE: To investigate whether mast cell chymase is involved in burn injury and the change of chymase expression in burn wound.
METHODS: Deep partial-thickness scald in hamsters was induced with contact time of 12 seconds by 20 mL 75 ℃ water in a   50 mL syringe. Chymase mRNA level and chymase activity were analyzed before burn and at 1, 3, 7, 14 days after burn in deep second-degree scald wound.
RESULTS AND CONCLUSION: Quantitative real-time RT-PCR and radioimmunoassay results showed that chymase gene expression and chymase activity in burn wound were significantly increased after burn and were highest at 3 days after burn. Expression of mast cell chymase would be involved in burn injury and changed at different stages after burn in hamsters.

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