中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8613-8616.doi: 10.3969/j.issn.1673-8225.2011.46.015

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

同基因背景小鼠核蛋白诱导狼疮鼠模型

谢  君，林有坤   

  1. 广西医科大学第一附属医院，广西壮族自治区南宁市  530021
  • 收稿日期:2011-04-02 修回日期:2011-04-12 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 林有坤，硕士生导师，主任医师，教授，广西医科大学第一附属医院，广西壮族自治区南宁市 530021 linyoukun7@yahoo.com.cn
  • 作者简介:谢君★，女，1983年生，湖北省武汉市人，汉族，2009年毕业于广西医科大学，硕士，住院医师，主要从事自身免疫性皮肤病研究。 xiejunz@sina.com
  • 基金资助:

    广西青年科学基金资金资助项目(桂科青0542055)；广西卫生厅重点科技项目(桂卫重200829)。

A mouse model of lupus induced by nucleoprotein with the same genetic background

Xie Jun, Lin You-kun   

  1. Department of Dermatovenereology, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-02 Revised:2011-04-12 Online:2011-11-12 Published:2011-11-12
  • Contact: Lin You-kun, Master’s supervisor, Chief physician, Professor, Department of Dermatovenereology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China linyoukun7@yahoo.com.cn
  • About author:Xie Jun★, Master, Physician, Department of Dermatovenereology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China xiejunz@sina.com
  • Supported by:

    Youth Science Foundation of Guangxi Zhuang Autonomous Region, No. guikeqing0542055*; A Key Science and Technology Program of Guangxi Department of Health, No. Guiweizhong 200829*

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摘要：

背景：自发性狼疮鼠模型不能对基因以外其他的致病因素进行研究。
目的：以同基因背景Balb/c小鼠核蛋白免疫小鼠后诱导狼疮鼠模型。
方法：选取4~6周SPF级Balb/c小鼠30只，等分为3组。V1组肌肉注射提取的同系Balb/c小鼠核蛋白，间隔3周免疫1次，共免疫4次；V2组注射等体积PBS；V3组为正常对照。检测小鼠末次免疫后3周的24 h尿蛋白、血清抗核抗体、抗双链DNA抗体、小鼠肾脏直接免疫荧光。
结果与结论：V1组小鼠24 h尿蛋白、血清抗双链DNA抗体、抗核抗体均明显高于V2组、V3组，且V1组小鼠肾小球有免疫球蛋白G免疫复合物沉积，可见肾小球轮廓，V2组、V3组未见肾小球轮廓，只见非特异性的微弱荧光。说明以同基因背景Balb/c小鼠核蛋白免疫Balb/c小鼠能够成功诱导狼疮鼠模型。

关键词: 狼疮鼠, 核蛋白, 免疫, 模型鼠, 系统性红斑狼疮

Abstract:

BACKGROUND: A mouse model of spontaneous lupus cannot be used for study of pathogenic factors other than genetic factors.
OBJECTIVE: To establish a mouse model of lupus induced by Balb/c mouse nucleoprotein with the same genetic background.
METHODS: Thirty Balb/c mice of SPF grade, aged 4-6 weeks, were randomly and evenly divided into three groups. The V1 group mice were intramuscularly administered nucleoprotein extracted from the same strains of Balb/c mice, once every other 3 weeks, for a total of four times. The V2 group mice were administered the same volume of phosphate buffered saline. The V3 group mice served as normal controls. The 24-hour urine protein, serum antinuclear antibody, anti-ds-DNA antibody and direct immunofluorescence of mouse kidney were detected at 3 weeks after the last immunization.
RESULTS AND CONCLUSION: The 24-hour urine protein, anti-ds-DNA antibody, and antinuclear antibody in the V1 group were significantly higher than those in the V2 and V3 groups. There was IgG immune complex deposition in the renal glomerulus of V1 group. However, in the V2 and V3 groups, renal glomerulus contour was not observed, and only non-specific fluorescence could be observed. A mouse model of lupus can be successfully induced by immunizing Balb/c mouse with Balb/c mouse nucleoprotein with the same genetic background.

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