中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8591-8594.doi: 10.3969/j.issn.1673-8225.2011.46.010

• 骨组织构建 bone tissue construction • 上一篇    下一篇

低氧条件下成骨细胞的增殖与分化

高文魁，王德元，李智钢，闫自强，白  峰，王  伟   

  1. 解放军第四医院骨科，青海省西宁市  810007
  • 收稿日期:2011-07-28 修回日期:2011-08-26 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 王伟，硕士，主治医师，解放军第四医院骨科，青海省西宁市 810007 warerry@163.com
  • 作者简介:高文魁★，男，1953年生，宁夏回族自治区中卫市人，汉族，1991年解放军第四军医大学毕业，硕士，主任医师，主要从事骨外科学、骨质疏松研究。 osteo4pla@163.com
  • 基金资助:

    青海省科学技术厅科技创新能力促进计划项目(2010.Z.744)，课题名称：低氧环境在骨质疏松发生发展过程中的作用机制研究。 

Effects of hypoxia on proliferation and differentiation of rat osteoblasts

Gao Wen-kui, Wang De-yuan, Li Zhi-gang, Yan Zi-qiang, Bai Feng, Wang Wei   

  1. Department of Orthopedics, the Fourth Hospital of Chinese PLA, Xining 810007, Qinghai Province, China
  • Received:2011-07-28 Revised:2011-08-26 Online:2011-11-12 Published:2011-11-12
  • Contact: Wang Wei, Master, Attending physician, Department of Orthopedics, the Fourth Hospital of Chinese PLA, Xining 810007, Qinghai Province, China warerry@163.com
  • About author:Gao Wen-kui★, Master, Chief physician, Department of Orthopedics, the Fourth Hospital of Chinese PLA, Xining 810007, Qinghai Province, China osteo4pla@163.com
  • Supported by:

    Science and Technology Innovation Ability Promotion Plan Program of Department of Science and Technology of Qinghai Province, No. 2010.Z.744*

PDF

318

被引次数

21

摘要：

背景：低氧可通过多种途径作用于成骨细胞影响骨代谢，对骨生成、骨愈合等产生负面影响。
目的：观察低氧对体外培养大鼠成骨细胞增殖、分化的影响，并探讨其分子机制。
方法：分离培养新生Wistar大鼠颅盖骨成骨细胞，取第2代细胞分别在常氧(体积分数20%O2)与低氧(体积分数3%O2)条件下培养。
结果与结论：低氧组成骨细胞增殖、碱性磷酸酶活性、骨钙素水平及茜素红结节形成数量均明显低于常氧组(P < 0.05或  P < 0.01)，说明缺氧条件对成骨细胞的增殖、分化及功能有抑制作用；低氧组骨形成发生蛋白2及Runx2表达低于常氧组(P < 0.05或P < 0.01)，说明低氧条件下大鼠成骨细胞Runx2、骨形成发生蛋白2的表达受抑制。结果表明低氧可通过抑制大鼠成骨细胞的Runx2、骨形成发生蛋白2的表达进一步抑制成骨细胞的增殖与分化。

关键词: 低氧, 成骨细胞, 增殖, 分化, Runx2, 骨形成发生蛋白2

Abstract:

BACKGROUND: Hypoxia influences bone metabolism by many means and exhibits negative effects on bone generation and bone healing.
OBJECTIVE: To explore the effects of hypoxia on proliferation and differentiation of rat osteoblasts and investigate the underlying mechanisms.
METHODS: Primary rat osteoblasts were isolated from excised calvarial bones of neonatal mice and cultured in vitro. The second passage of osteoblasts were cultured under hypoxic (3% O2) and normoxic (20% O2) conditions.
RESULTS AND CONCLUSION: The proliferation levels, alkaline phosphatase activities, osteocalcin contents and the number of calcium nodules in the hypoxic group were significantly lower compared with the normoxic group (P < 0.05 or P < 0.01). It means that hypoxia inhibits the proliferation, differentiation and functions of rat osteoblasts. The mRNA expression of bone morphogenetic protein-2 and Runx2 in osteoblasts cultured in 3% O2 was lower than that cultured in 20% O2 ( P < 0.05 or      P < 0.01). It means that the mRNA expression of Runx2 and bone morphogenetic protein-2 was decreased in hypoxic conditions. These findings suggest that hypoxia can inhibit the proliferation and differentiation of rat osteoblasts through inhibiting Runx2 and BMP-2 mRNA expression in vitro.

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