中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8451-8454.doi: 10.3969/j.issn.1673-8225.2011.45.020

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

脑缺血再灌注海马齿状回神经干细胞Mash-1的表达

吕威力1，邢雪松2，张  玲1，张  岷1   

  1. 沈阳医学院，1 病理学教研室，2解剖学教研室，辽宁省沈阳市 110034
  • 收稿日期:2011-04-15 修回日期:2011-06-13 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 邢雪松，博士，副教授，沈阳医学院解剖学教研室，辽宁省沈阳市 110034 syyxyx@sina.com
  • 作者简介:吕威力☆，女，1974年生，辽宁省沈阳市人，2006年中国医科大学毕业，博士，副教授，主要从事神经干细胞增殖分化机制及基因调控的研究。
  • 基金资助:

    辽宁省教育厅科学研究计划资助项目(L2010546)。

Expression of Mash-1 in hippocampal neural stem cells after cerebral ischemia/reperfusion injury in rats

Lü Wei-li1, Xing Xue-song2, Zhang Ling1, Zhang Min1   

  1. 1Department of Pathology, 2Department of Anatomy, Shenyang Medical College, Shenyang  110034, Liaoning Province, China
  • Received:2011-04-15 Revised:2011-06-13 Online:2011-11-05 Published:2011-11-05
  • Contact: Xing Xue-song, Doctor, Associate professor, Department of Anatomy, Shenyang Medical College, Shenyang 110034, Liaoning Province, China syyxyx@sina.com
  • About author:Lü Wei-li☆, Doctor, Associate professor, Department of Pathology, Shenyang Medical College, Shenyang 110034, Liaoning Province, China
  • Supported by:

    the Scientific Research Program of Liaoning Education Bureau, No. L2010546*

PDF

267

被引次数

3

摘要：

背景：Mash-1在中枢神经系统和周围神经系统发育中都有表达，对神经元和神经胶质细胞的分化都有很重要的作用。
目的：检测Mash-1在大鼠脑缺血再灌注海马组织神经干细胞中的表达及其变化。
方法：建立大脑中动脉栓塞制作大鼠脑缺血再灌注模型。实验分为假手术组、缺血再灌注3，7，14，21，28 d组。
结果与结论：免疫组织化学检测结果，随着脑缺血再灌注第3天海马神经元BrdU阳性细胞明显增多，第7天达高峰，然后逐渐减少。Mash-1反应产物随脑缺血再灌注时间延长逐渐增多，第21天表达最强，以后表达逐渐减少。提示Mash-1呈时间依赖性表达，与神经干细胞增殖分化进程相吻合，说明其在神经干细胞晚期分化中起重要调控作用。

关键词: Mash-1, 神经干细胞, 增殖, 分化, 大鼠

Abstract:

BACKGROUND: Mash-1 is expressed during the development of the central nervous system and peripheral nervous system, and plays an important role in neurons and glial cell differentiation.
OBJECTIVE: To investigate the expression of Mash-1 in the hippocampus after cerebral ischemia/reperfusion in rats.
METHODS: A novel model of cerebral ischemia/reperfusion was established with the method of middle cerebral artery occlusionin rats. The rats were divided into sham-operation group, and ischemia/reperfusion groups of 3, 7, 14, 21 and 28 days.
RESULTS AND CONCLUSION: After 3 days, BrdU positive cells in the hippocampus were obviously increased. To the 7th day, BrdU positive cells were more than those at any time. Mash-1 reaction products were increased gradually with the time of cerebral ischemia/reperfusion, reached the peak on day 21, and then gradually decreased. The expression of Mash-1 is in a time-depended manner and corresponds to the proliferation and differentiation of neural stem cells, suggesting that Mash-1 can play the important effect in regulating the neural stem cell differentiation in the late stage.

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