中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (40): 7480-7484.doi: 10.3969/j.issn.1673-8225.2011.40.016

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

脂肪干细胞与外源性血管内皮生长因子及纤维蛋白胶复合物体内构建血管化组织工程脂肪

黎洪棉1， 2，高建华3，吴  涛4，赵培冉4，梁双武4   

  1. 1中山大学附属中山医院博士后科研工作站、整形美容外科，广东省中山市  528403
    2 南方医科大学博士后科研流动站，广东省广州市  510515
    3南方医科大学南方医院整形外科，广东省广州市 510515
    4南方医科大学组织工程研究中心，广东省广州市 510515
  • 收稿日期:2011-04-01 修回日期:2011-05-15 出版日期:2011-10-01 发布日期:2011-10-01
  • 通讯作者: 高建华，主任医师，教授，博士生导师，南方医科大学南方医院整形外科，广东省广州市510515 gaopsnf@yahoo.com
  • 作者简介:黎洪棉☆，男，1971年生，广西壮族自治区贺州市人，汉族，博士后，副主任医师，硕士生导师，主要从事整形美容外科和干细胞、组织工程与再生医学方面的研究。 binrong2112@163.com
  • 基金资助:

    中国博士后科学基金(20090450910)；广东省医学科研基金项目(A2011739)，中山市科技计划项目(20113A008)。

In vivo construction of vascularized tissue engineering adipose via adipose-derived stem cells and exogenous vascular endothelial growth factor carrier complex in vivo

Li Hong-mian1, 2, Gao Jian-hua3, Wu Tao4, Zhao Pei-ran4, Liang Shuang-wu4   

  1. 1Postdoctoral Mobile Work Station, Department of Plastic and Aesthetic Surgery, the Affiliated Zhongshan Hospital of Sun Yat-sen University, Zhongshan  528403, Guangdong Province, China
    2Postdoctoral Mobile Research Station, Southern Medical University, Guangzhou  510515, Guangdong Province, China
    3Department of Plastic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou  510515, Guangdong Province, China
    4Research Center of Tissue Engineering, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2011-04-01 Revised:2011-05-15 Online:2011-10-01 Published:2011-10-01
  • Contact: Gao Jian-hua, Chief physician, Professor, Doctoral supervisor, Department of Plastic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China gaopsnf@yahoo.com
  • About author:Li Hong-mian☆, Doctor, Associate chief physician, Master’s supervisor, Postdoctoral Mobile Work Station, Department of Plastic and Aesthetic Surgery, the Affiliated Zhongshan Hospital of Sun Yat-sen University, Zhongshan 528403, Guangdong Province, China; Postdoctoral Mobile Research Station, Southern Medical University, Guangzhou 510515, Guangdong Province, China binrong2112@163.com
  • Supported by:

    China Postdoctoral Foundation, No. 20090450910*; the Medical Science Pesearch Foundation of Guangdong Province, No.A2011739*; the Science and Technology Plan Program of Zhongshan City, No.20113A008*

PDF

521

被引次数

17

摘要：

背景：血运重建机制是组织工程化脂肪组织成功构建的决定性因素。
目的：观察脂肪干细胞与外源性血管内皮生长因子和纤维蛋白胶复合物在体内构建血管化组织工程脂肪的可行性。
方法：从健康成年人吸脂术后的脂肪组织中分离脂肪干细胞并行原代及传代培养，将第3代经BrdU标记的脂肪干细胞向脂肪细胞定向诱导2周后，制成5×1010 L-1细胞悬液。由0.5 mL细胞悬液、100 μL的血管内皮生长因子工作液或DMEM培养基与0.5 mL纤维蛋白胶组成实验组和对照组移植物，分别植入裸鼠背部皮下。
结果与结论：术后8周取材时：①实验组可见血管增生并长入材料，呈轻度纤维包裹；对照组有少量血管长入材料中，也有轻度纤维包裹现象。实验组新生组织湿质量大于对照组(P < 0.01)。②苏木精-伊红染色均可见移植物中有新生脂肪组织形成和不同程度的微血管长入；实验组微血管数多于对照组(P < 0.01)。③新生组织免疫荧光染色示，两组新生脂肪细胞的胞核及部分微血管内皮细胞的胞核呈现绿色荧光。结果说明脂肪干细胞与外源性血管内皮生长因子和纤维蛋白胶复合物在体内可构建血管化组织工程脂肪，脂肪干细胞与外源性血管内皮生长因子共同参与新生脂肪组织的血管化过程。

关键词: 人脂肪干细胞, 血管内皮生长因子, 纤维蛋白胶, 组织工程脂肪, 血管发生

Abstract:

BACKGROUND: Revascularization mechanism is the decisive factor for the successful construction of tissue engineering adipose tissue.
OBJECTIVE: To observe the feasibility of construction of vasuclarized tissue engineering adipose via adipose-derived stem cells and exogenous vascular endothelial growth factor (VEGF) carrier complex in vivo.
METHODS: Adipose derived stem cells (ADSCs) were isolated from the subcutaneous adipose tissue of healthy adult after liposuction, and primary culture and subculture of ADSCs were conducted. After being induced towards adipocytes for 2 weeks, 5× 1010/L passage 3 cell suspension labeled by BrdU was prepared. The experiment was divided into two groups: group A (experimental group) complex in which 0.5 mL cell suspension, 100 μL VEGF and 0.5 mL fibrin glue were implanted into the subcutaneous fascia of nude mice; group B (control group) complex in which 0.5 mL cell suspension, 100 μL DMEM and 0.5 mL fibrin glue were implanted into the subcutaneous fascia of nude mice.
RESULTS AND CONCLUSION: Eight weeks after operation, neogenetic vessels grew into the scaffolds and mild fiber encapsulation was observed in group A, while few vessels grew into the scaffolds and also mild fiber encapsulation was observed in group B. The wet weight of cambium in group A was higher than that in group B (P < 0.01). Hematoxylin-eosin staining showed the formation of neogenetic adipose tissues and the growth of micrangium in the implant. The number of micro vessels in group A was greater than that in group B (P < 0.01). The immunofluorescence staining of cambium showed that the cell nucleus of regenerated adipocytes and partial capillary endothel ium in groups A and B presented green fluorescence. It is feasible to prepare vasuclarized tissue engineering adipose via ADSCs and exogenous VEGF carrier complex in vivo. ADSCs and exogenous VEGF participate in neovascularization of neogenetic adipose tissue.

中图分类号: