中国组织工程研究

中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (25): 4046-4053.doi: 10.3969/j.issn.2095-4344.2092

• 干细胞综述 stem cell review • 上一篇    下一篇

内皮祖细胞促进血管发生及新骨生成辅助治疗骨缺损

冯  源1，2，3，4，韩志琪1，2，3，4，周  诺1，2，3，4   

  1. 1广西医科大学口腔医学院/附属口腔医院；2广西口腔颌面修复与重建研究自治区级重点实验室；3广西颅颌面畸形临床医学研究中心；4颌面外科疾病诊治研究重点实验室
  • 收稿日期:2019-12-02 修回日期:2019-12-06 接受日期:2020-01-22 出版日期:2020-09-08 发布日期:2020-08-26
  • 通讯作者: 周诺，博士，教授，博士生导师，广西医科大学口腔医学院/附属口腔医院，广西壮族自治区南宁市 530021；广西口腔颌面修复与重建研究自治区级重点实验室，广西壮族自治区南宁市530021；广西颅颌面畸形临床医学研究中心，广西壮族自治区南宁市530021；颌面外科疾病诊治研究重点实验室(广西高校重点实验室)，广西壮族自治区南宁市530021
  • 作者简介:冯源，女，1994年生，新疆维吾尔自治区博乐市人，汉族，广西医科大学附属口腔医院在读硕士，主要从事颌骨牵张成骨机制的研究
  • 基金资助:

    国家自然科学基金(81870748)；广西医疗卫生适宜技术研究与开发项目(S201538)；广西医疗卫生重点科研课题(桂卫重200525)

Endothelial progenitor cells promote vasculogenesis and osteogenesis in the repair of bone defects   

Feng Yuan1, 2, 3, 4, Han Zhiqi1, 2, 3, 4, Zhou Nuo1, 2, 3, 4    

  1. 1College of Stomatology, Guangxi Medical University; 2Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction; 3Guangxi Clinical Research Center for Craniofacial Deformity; 4Guangxi Key Laboratory of Oral and Maxillofacial Surgery
  • Received:2019-12-02 Revised:2019-12-06 Accepted:2020-01-22 Online:2020-09-08 Published:2020-08-26
  • Contact: Zhou Nuo, MD, Professor, Doctoral supervisor, College of Stomatology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Feng Yuan, Master candidate, College of Stomatology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81870748; Technology Research and Development Program for Health Care in Guangxi Zhuang Autonomous Region, No. S201538; Key Scientific Research Program for Health Care in Guangxi Zhuang Autonomous Region, No. 200525

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摘要：

文题释义：

内皮祖细胞：是血管内皮细胞的前体细胞，可在生理或病理因素刺激下，从骨髓动员到外周血参与损伤血管的修复，在血管内皮再生中发挥着重要作用。

血管发生：在胚胎发育时从中胚层起始，由成血管细胞发育成血管内皮细胞并最终形成血管的过程，这是血管“从无到有”的一个生物学过程。

 

摘要

背景：骨折是临床常见的骨科疾病，虽然大多数骨折可以通过坚强内固定等手术治疗实现一期愈合，但仍有相当比例的骨折愈合不良，最终导致骨缺损，而骨缺损部位的血管新生对骨缺损的修复起到至关重要的作用。基于内皮祖细胞促进组织血管发生的能力，其对骨再生和修复的辅助作用逐渐成为学界关注的焦点。

目的：综述骨缺损疾病中使用内皮祖细胞促进血管发生及新骨生成的研究进展。

方法：遵循PRISMA指南，检索CNKI、万方数据库和PubMed数据库1986至2019年期间关于内皮祖细胞辅助治疗骨缺损的动物实验、临床试验及其机制的文章，检索词分别为“内皮祖细胞，血管生成，血管发生，治疗，骨缺损”“endothelial progenitor cells (EPCs)，angiogenesis，vasculogenesis，therapy/treatment，bone defect”，最后选择58篇文章纳入结果分析。

结果与结论：①骨缺损时可以促进内皮祖细胞的激活与归巢；②内皮祖细胞可以促进血管发生；③内皮祖细胞是通过促进血管发生进而促进骨再生的；④将内皮祖细胞辅助治疗骨缺损推广至临床应用目前尚存在一些问题有待解决；⑤需要通过进行更多的临床试验，并且进一步深入研究内皮祖细胞参与血管发生的确切机制，为将来的临床应用提供数据支持，给大量骨缺损致残患者带来更好的治疗。

ORCID: 0000-0001-5481-3865(冯源)

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词:

内皮祖细胞, 骨缺损,  干细胞移植,  组织工程技术,  血管发生,  骨生成

Abstract:

BACKGROUND: Fracture is a common orthopedic disease in clinic. Although most fractures can heal by primary bone healing through surgical treatments, such as rigid internal fixation, malunion is liable to occur in some circumstances, eventually leading to bone defects. Neovascularization at the bone defect site plays a vital role in bone healing. Based on the pro-angiogenic ability of endothelial progenitor cells (EPCs), its capacity for bone regeneration and repair has gradually become the focus of attention.

OBJECTIVE: To review the use of EPCs transplantation to promote vasculogenesis and osteogenesis in bone defects.

METHODS: With the key words of “endothelial progenitor cells (EPCs), angiogenesis, vasculogenesis, therapy/treatment, bone defect” in Chinese and English, we performed a computer-based search in CNKI, WanFang and PubMed databases for relevant articles published from 1986 to 2019. Finally, 58 eligible articles were included in result analysis. During the literature retrieval, we followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses.

RESULTS AND CONCLUSION: Bone defects can promote the activation and homing of EPCs. EPCs can promote vasculogenesis and further trigger bone regeneration. There are still some problems to be solved before the application of EPCs in the treatment of bone defects. More experiments need to be carried out to investigate the exact mechanism of EPCs in vasculogenesis. More clinical trials are warranted, providing data support for future clinical applications and giving better treatment to patients disabled by bone defects.

Key words:

endothelial progenitor cells,  bone defect,  stem cell transplantation,  tissue engineering technology,  vasculogenesis,  osteogenesis 

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