中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6947-6950.doi: 10.3969/j.issn.1673-8225.2011.37.025

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

不同葡萄糖培养条件对L6骨骼肌细胞中活性氧的生成作用

庞  婧1, 2，代  杨3，宫  环1, 2，张恩毅1, 2，张铁梅1, 2   

  1. 1卫生部北京医院，北京市 100730
    2卫生部北京老年医学研究所，北京市 100730
    3中国医学科学院北京协和医学院，北京市 100730
  • 收稿日期:2011-02-19 修回日期:2011-04-25 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 张铁梅，博士，研究员，卫生部北京医院老年医学研究所，北京市 100730 tmzhang126@ 126.com
  • 作者简介:庞婧☆，女，1982年生，辽宁省沈阳市人，汉族，2010年中国医学科学院北京协和医学院毕业，博士，助理研究员，主要从事2型糖尿病分子机制的研究。 pangjing314@ 163.com
  • 基金资助:

    国家自然科学基金面上项目(30971395)，课题名称：多因素综合作用下的血糖变化趋势判定研究。

Reactive oxygen species formation induced by different concentrations of glucose in L6 cells

Pang Jing1, 2, Dai Yang3, Gong Huan1, 2, Zhang En-yi1, 2, Zhang Tie-mei1, 2   

  1. 1Beijing Hospital, Ministry of Health, Beijing  100730, China
    2Beijing Institute of Geriatrics, Ministry of Health, Beijing  100730, China
    3Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing  100730, China
  • Received:2011-02-19 Revised:2011-04-25 Online:2011-09-10 Published:2011-09-10
  • Contact: Zhang Tie-mei, Doctor, Researcher, Beijing Hospital, Ministry of Health, Beijing 100730, China; Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China tmzhang126@126. com
  • About author:Pang Jing☆, Doctor, Assistant Researcher, Beijing Hospital, Ministry of Health, Beijing 100730, China; Beijing Institute of Geriatrics, Ministry of Health, Beijing 100730, China
  • Supported by:

    the National Natural Science Foundation of China (General Program), No.30971395*

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摘要：

背景：活性氧在不同葡萄糖培养条件下的生成状况是研究2型糖尿病发病机制的基础。
目的：观察不同浓度葡萄糖培养条件下，细胞内活性氧的生成情况。
方法：取体外培养的L6骨骼肌细胞和诱导分化的肌管细胞，分别用含有3，5.5，25 mmol/L葡萄糖的DMEM培养液培养，检测细胞内活性氧的生成情况。
结果与结论：随着高糖培养时间的延长，细胞内活性氧生成有增加的趋势，其中25 mmol/L葡萄糖培养细胞4 d的活性氧生成明显高于5.5 mmol/L葡萄糖培养细胞的水平；3，5.5 mmol/L葡萄糖培养对细胞内活性氧生成无显著影响。诱导肌管细胞较成肌细胞在高糖培养条件下更易产生活性氧。说明体外培养细胞葡萄糖浓度、培养时间和培养细胞分化程度是影响细胞内活性氧生成的因素，可用于模拟体内高糖损伤的积累效应。

关键词: 高糖, 低糖, 活性氧, 骨骼肌细胞, 肌管细胞, 损伤

Abstract:

BACKGROUND: Reactive oxygen species (ROS) formation induced by treatment with different culture conditions of glucose in L6 cells is the basis for studying the pathogenesis of type 2 diabetes mellitus.
OBJECTIVE: To investigate the generation of intracellular ROS induced by treatment with different concentrations of glucose and different culture time periods.
METHODS: L6 skeletal muscle cells cultured in vitro and L6 myotube cells differentiated were treated with DMEM containing 3, 5.5 and 25 mmol/L glucose and ROS formation was determined.
RESULTS AND CONCLUSION: With prolonged culture time, intracellular ROS formation increased. ROS formation was significantly accumulated in cells treated with 25 mmol/L glucose than in cells treated with 5.5 mmol/L glucose by day 4. Glucose at a concentration of 3 and 5.5 mmol/L did not produce significant effects on ROS formation. ROS formation in L6 myotubes cultured by high concentration of glucose was more obvious than that in L6 mysoblast. Results showed that glucose concentration, culture time and the differentiation degree of in vitro cultured cells are the factors influencing ROS formation and in vitro culture of cells by glucose can be used to mimic the chronic damage effect of hyperglycemia in vivo.

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