中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6951-6955.doi: 小胶质细胞|姜黄素|Notch1|剂量依赖|组织工程

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

不同浓度姜黄素对体外培养小胶质细胞过度活化的抑制作用

金玉玲1，罗海龙2，刘  蕾3，程春凤4，侯丽淳1   

  1. 1 佳木斯大学附属第一医院神经科，黑龙江省佳木斯市  154002
    2 牡丹江医学院红旗医院神经内科，黑龙江省牡丹江市  157011
    3 佳木斯大学基础医学院病生教研室，黑龙江省佳木斯市  154007
    4佳木斯大学附属第二医院神经科，黑龙江省佳木斯市  154003
  • 收稿日期:2011-03-04 修回日期:2011-04-25 出版日期:2011-09-10 发布日期:2011-09-10
  • 作者简介:金玉玲★，女，1969年生，黑龙江省密山市人，汉族，2001年佳木斯大学毕业，硕士，副主任医师，主要从事神经干细胞基础与应用方面的研究。 29757978@qq.com
  • 基金资助:

    黑龙江省自然科学基金项目(D200859)。

Inhibitory effects of different concentrations of curcumin on excessive activation of microglia cultured in vitro

Jin Yu-ling1, Luo Hai-long2, Liu Lei3, Cheng Chun-feng4, Hou Li-chun1   

  1. 1Department of Neurology, First Affiliated Hospital, Jiamusi University, Jiamusi  154002, Heilongjiang Province, China
    2Department of Neurology, Hongqi Hospital of  Mudanjiang Medical College, Mudanjiang  157011, Heilongjiang Province, China
    3Department of Pathophysiology, School of Basic Medical Sciences, Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
    4Department of Neurology, Second Hospital Affiliated to Jiamusi University, Jiamusi  154003, Heilongjiang Province, China
  • Received:2011-03-04 Revised:2011-04-25 Online:2011-09-10 Published:2011-09-10
  • About author:Jin Yu-ling★，Master, Associate chief physician, Department of Neurology, First Affiliated Hospital, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China 29757978@qq. com
  • Supported by:

    the Natural Science Foundation of Heilongjiang Province, No. D200859*  

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被引次数

1

摘要：

背景：过度活化的小胶质细胞会释放大量的神经毒性因子损伤神经功能。
目的：观察不同浓度的姜黄素对体外培养小胶质细胞过度活化的抑制作用。
方法：取新生48 h内Wistar大鼠用于小胶质细胞的提取及原代培养，分别加入1，5，20 μmol/L的姜黄素抑制3，7 d，观察细胞形态，CD11b/c的表达，Notch1 mRNA的表达。
结果与结论：原代培养的小胶质细胞折光性强，以圆形多见，呈过度活化状态。姜黄素干预后静止细胞阳性率明显升高，在培养第7天时阳性率达到高峰。RT-PCR结果显示，1，5，20 μmol/L姜黄素都可明显抑制小胶质细胞Notch1 mRNA的表达。以5 μmol/L姜黄素的抑制效果最显著(P < 0.01)。说明姜黄素对体外培养的小胶质细胞过度活化的抑制作用存在剂量依赖性。

关键词: 小胶质细胞, 姜黄素, Notch1, 剂量依赖, 组织工程

Abstract:

BACKGROUND: Excessively activated microglia can release a large number of neurotoxic factors which damage nerve function.
OBJECTIVE: To investigate the inhibitory effects of different concentrations of curcumin on excessive activation of microglia cultured in vitro.
METHODS: Microglias were extracted from neonatal (within 48 hours) Wistar rats and cultured. At 3 and 7 days after addition of different concentrations of curcumin (1, 5, 20 μmol/L), cell morphology, CD11b/c expression and Notch1 mRNA expression were determined.
RESULTS AND CONCLUSION: The primary cultured microglia exhibited strong refractive capacity and a spherical morphology and were excessively activated. After intervention of curcumin, the positive rate of static cells was obviously increased, and the positive rate reached a peak on day 7 of culture. RT-PCR showed that 1, 5, 20 μmol/L curcumin can obviously inhibit Notch1 mRNA expression in the microglia, and 5 μmol/L curcumin showed the most obvious inhibitory effects (P < 0.01). These results suggest that curcumin shows a dose-dependent inhibitory effect on excessive activation of microglia cultured in vitro.

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