中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (35): 6571-6574.doi: 10.3969/j.issn.1673-8225.2011.35.026

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

地塞米松联合脂多糖诱导股骨头坏死模型的构建

田  力1，梁晓鹏2，田晓晔3，于鑫琛4，田  晶4   

  1. 沈阳医学院，1临床教研室，2眼视光学院，辽宁省沈阳市   110034；3铁岭经济开发区靖康康复养老中心，辽宁省铁岭市 112000；4沈阳靖康医疗集团，辽宁省沈阳市 110043
  • 收稿日期:2011-02-28 修回日期:2011-02-28 出版日期:2011-08-27 发布日期:2011-08-27
  • 通讯作者: 梁晓鹏，硕士，工程师，沈阳医学院眼视光学院，辽宁省沈阳市 110034 Linan1956@yahoo.com.cn
  • 作者简介:田力，男，1956年生，河北省秦皇岛市人，汉族，教授，主任医师，从事临床外科、局解手术学的临床、教学和科研工作。 Linan1956@yahoo.com.cn
  • 基金资助:

    辽宁省教育厅基金资助项目(2008-7-29)；沈阳科技局基金资助项目(1081233-1-00-03)。

Constructing a rabbit model of necrosis of the femoral head by the combination of dexamethasone and lipopolysaccharide

Tian Li1, Liang Xiao-peng2, Tian Xiao-ye3, Yu Xin-chen4, Tian Jing4   

  1. 1Clinical Department, 2School of Optometry, Shenyang Medical College, Shenyang  110034, Liaoning Province, China; 3Jingkang Rehabilitation Pension Center, Tieling Economic Development Zone, Tieling  112000, Liaoning Province, China; 4Shenyang Jingkang Medical Group, Shenyang  110043, Liaoning Province, China
  • Received:2011-02-28 Revised:2011-02-28 Online:2011-08-27 Published:2011-08-27
  • Contact: Liang Xiao-peng, Master, Engineer, School of Optometry, Shenyang Medical College, Shenyang 110034, Liaoning Province, China Linan1956＠yahoo.com.cn
  • About author:Tian Li, Professor, Chief physician, Clinical Department, Shenyang Medical College, Shenyang 110034, Liaoning Province, China Linan1956@yahoo.com.cn
  • Supported by:

    the Foundation of Liaoning Educational Bureau, No. 2008-7-29*; the Funding Program of Shenyang Science and Technology Department, No. 1081233-1-00-03*

PDF

353

被引次数

18

摘要：

背景：对于研究股骨头坏死的发病机制及治疗方法，建立与股骨头坏死病程相似的动物模型是非常重要的。
目的：观察地塞米松联合脂多糖诱导兔股骨头坏死的实验效果。
方法：健康雄性新西兰大白兔30只，随机分为模型组(n=20)和对照组(n=10)。模型组经耳缘静脉注射2次10 μg/kg的脂多糖，再肌肉注射3次地塞米松25 mg/kg，对照组注射同等剂量的生理盐水。
结果与结论：4周后，CT扫描显示模型组兔股骨头不同程度的骨密度不均匀。显微CT观察发现，模型组骨体积分数、骨矿物质密度、骨矿物质含量和骨小梁厚度均显著低于对照组，而骨小梁间隙高于对照组(P < 0.05)。组织学切片见模型组骨细胞陷窝空疏，脂肪细胞增多，部分血管栓塞，其中存活动物的骨坏死率和骨陷窝空虚率均明显低于对照组。说明地塞米松联合脂多糖可有效建立骨坏死模型。

关键词: 股骨头坏死, 地塞米松, 脂多糖, 显微CT, 组织学切片

Abstract:

BACKGROUND: An ideal animal model of necrosis of the femoral head is beneficial to the studies on the cause of disease, pathogenesis and treatment.
OBJECTIVE: To research the experimental result of necrosis of rabbit femoral head induced by the combination of dexamethasone and lipopolysaccharide.
METHODS: Thirty male New Zealand rabbits were divided into experimental group (20 rabbits) and control group (10 rabbits) randomly. Two injections of 10 μg/kg body lipopolysaccharide and three injections of 25 mg/kg dexamethasone were performed in the experimental group. Physiologic saline was injected in the control group.
RESULTS AND CONCLUSION: The CT scanning showed non-uniform of bone density of the femoral head in the experimental group. The indexes of Micro-CT bone metrological parameters were significantly lower in the experimental group than in the control group (P < 0.05). Bone cell lacunae, increased fat cells and thrombokinesis were observed in histopathological examination. The osteonecrosis rate of survived rabbits as well as the rate of empty bone lacunae was significantly lower in the experimental group than the control group. It is indicated that the combination of dexamethasone and lipopolysaccharide can induce an osteonecrosis model.

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