中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (34): 6385-6387.doi: 10.3969/j.issn.1673-8225.2011.34.028

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

Fe3O4磁性微粒的制备及表征

李  黎1，马  力2   

  1. 1成都农业科技职业学院，四川省成都市 611130
    2西华大学生物工程学院，四川省成都市 610039
  • 收稿日期:2011-05-24 修回日期:2011-07-08 出版日期:2011-08-20 发布日期:2011-08-20
  • 作者简介:李黎★，女，1982 年生，四川省成都市人，硕士研究生，主要从事食品生物技术研究。 lily112400@yahoo.cn

Preparation and characterization of Fe3O4 magnetic particles

Li Li1, Ma Li2   

  1. 1Chengdu Vocational College of Agricultural Science and Technology, Chengdu  611130, Sichuan Province, China
    2College of Bioengineering, XiHua University, Chengdu  610039, Sichuan Province, China
  • Received:2011-05-24 Revised:2011-07-08 Online:2011-08-20 Published:2011-08-20
  • About author:Li Li★, Studying for master’s degree, Chengdu Vocational College of Agricultural Science and Technology, Chengdu 611130, Sichuan Province, China lily112400@yahoo.cn

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摘要：

背景：磁性微粒作为一种磁性载体在固定化酶、免疫检测、靶向载药治疗及细胞分离等生物医学领域得到了广泛的应用。
目的：制备分散稳定性好，相对磁性强的纳米级Fe3O4微粒。
方法：以氯化亚铁、氯化铁、氢氧化钠为主要原料，采用化学共沉淀法合成Fe3O4磁性粒子。
结果与结论：用正交设计法优化了Fe3O4微粒的合成工艺条件，得到制备Fe3O4粒子的最佳实验条件为Fe2+/Fe3+的物质的量之比为2∶1、共沉淀时的pH值为11、熟化温度为90 ℃、表面活性剂聚乙二醇的用量为40 mL，此时制得的Fe3O4粒子粒径最小，为78 nm，Fe3O4溶液的分散稳定性最好，相对磁性最强。从Fe3O4的扫描电镜图可以看出，Fe3O4微粒晶体颗粒为纳米级。

关键词: 磁性微粒, Fe3O4, 生物材料, 磁流体, 纳米生物材料

Abstract:

BACKGROUND: As a kind of magnetic carrier, magnetic nanometer particles have been used in enzyme immobilization, immunoassay, target drug delivery and cell sorting and so on.
OBJECTIVE: To prepare nanometer Fe3O4 crystal particles with good dispersion stability and relatively strong magnetic.
METHODS: Fe3O4 magnetic particles was synthesized by the chemical co-precipitation, using FeCl2, FeCl3 and NaOH.
RESULTS AND CONCLUSION: The synthetic conditions of Fe3O4 magnetic particles were determined through orthogonal design and the optimum experimental conditions as follows: nFe2+/nFe3+ was 2:1, pH was 11, curing temperature was 90 ℃ and the amount of PEG was 40 mL. Under this condition, the average diameter of Fe3O4 particles was 78 nm, the dispersion stability was the best and the relative size of magnetic was the strongest. It could be seen from scanning electron microscope that Fe3O4 crystal particles were nanometer.

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