中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6168-6172.doi: 10.3969/j.issn.1673-8225.2011.33.022

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

合并中枢神经损伤骨折愈合过程中胰岛素样生长因子Ⅰ的作用

王  林   

  1. 泰山医学院附属医院，山东省泰安市  271000
  • 收稿日期:2011-02-12 修回日期:2011-03-20 出版日期:2011-08-13 发布日期:2011-08-13
  • 作者简介:王林★，男，1980年生，山东省泰安市人，汉族，2003年泰山医学院毕业，硕士，主治医师，主要从事骨创伤、组织缺损修复及显微外科的研究。 wltsmc@yahoo. cn

Role of insulin-like growth factor -Ⅰ in the healing of fracture complicated by central nervous system injury

Wang Lin   

  1. Affiliated Hospital of Taishan Medical University, Taian  271000, Shandong Province, China
  • Received:2011-02-12 Revised:2011-03-20 Online:2011-08-13 Published:2011-08-13
  • About author:Wang Lin★, Master, Attending physician, Affiliated Hospital of Taishan Medical University, Taian 271000, Shandong Province, China wltsmc@yahoo.cn

PDF

222

被引次数

10

摘要：

背景：胰岛素样生长因子Ⅰ由成骨细胞合成，并对成骨细胞具有促进增殖和分化作用。
目的：观察中枢神经损伤对胰岛素样生长因子Ⅰ在血清、局部骨痂的表达及骨折愈合的影响。
方法：取Wistar大鼠随机分成正常对照组、脑损伤-骨折组、脊髓损伤-骨折组、单纯骨折组。于术后第1，2，3周测量血清中胰岛素样生长因子Ⅰ水平；术后第2，3周行X射线摄片评估骨痂愈合情况，术后1，2，3，4周取股骨制作标本，测量骨痂体积，术后3 d，1，2周免疫组织化学染色法检测骨痂局部胰岛素样生长因子Ⅰ的表达。
结果与结论：正常对照组、单纯骨折组的胰岛素样生长因子Ⅰ血清质量浓度无明显变化，脑损伤-骨折组和脊髓损伤-骨折组骨痂体积、X射线评分高于单纯骨折组(P < 0.05)；伤后3 d， 1，2周时，脑损伤-骨折组和脊髓损伤-骨折组胰岛素样生长因子Ⅰ的血清质量浓度较其他两组明显增加(P < 0.01)；伤后1周，脑损伤-骨折组和脊髓损伤-骨折组骨折端平均阳性细胞数高于单纯骨折组(P < 0.01)。提示中枢神经损伤可以影响血清中胰岛素样生长因子Ⅰ的质量浓度和骨痂局部的表达，从而能促进骨折愈合。

关键词: 中枢神经损伤, 股骨骨折, 胰岛素样生长因子Ⅰ, 骨折愈合, 骨痂

Abstract:

BACKGROUND: Insulin-like growth factor -Ⅰ (IGF- Ⅰ) is synthesized by osteoblasts and has promotive effects on osteoblast proliferation and differentiation.
OBJECTIVE: To study the effect of central nerves system injury on expression of IGF- Ⅰ in blood serum, bony callus and fracture healing.
METHODS: Wistar rats were randomly divided into 4 groups: normal control group; simple fracture group; brain injury-fracture group; and spinal cord injury-fracture group. The level of IGF- Ⅰ in serum was measured at 1, 2 and 3 weeks after operation. Bony callus healing was evaluated by X-ray films at 2 and 3 weeks after operation. The femur was harvested at 1, 2, 3 and 4 weeks to measure the bony callus volume. The local expression of IGF- Ⅰin fracture sites were determined by immunohistochemical staining at 3 days, 1 and 2 weeks after operation.
RESULTS AND CONCLUSION: The serum IGF- Ⅰ in the normal group and simple fracture group was not changed greatly, but the bone callus volume and X-ray film grades of the brain injury-fracture group and spinal cord injury-fracture group were larger than those in the simple fracture group (P < 0.05). The concentrations of IGF- Ⅰ in the brain injury-fracture group and spinal cord injury-fracture group were increased significantly than those of other two groups at 3 days, 1 and 2 weeks after operation (P < 0.01); the count of positive cells in the brain injury-fracture group and spinal cord injury-fracture group were increased significantly than those of other two groups at 1 week after operation (P < 0.01). The central nervous injuries can increase the concentration of IGF- Ⅰ in blood serum and expression of bony callus, therefore, accelerates fracture healing in rats.

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