中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6161-6164.doi: 10.3969/j.issn.1673-8225.2011.33.020

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

改构型酸性成纤维细胞生长因子对衰老模型大鼠自由基与脂质过氧化物的影响

黄俊杰1，王彩冰1，何显教1，黄丽娟1，黄彦峰1，赵善民1，李倩茗2，黄巨恩3   

  1. 1右江民族医学院应用生理研究室，广西壮族自治区百色市533000；广西医科大学，2生理学教研室，3组胚学教研室，广西壮族自治区南宁市 530021
  • 收稿日期:2011-04-09 修回日期:2011-07-09 出版日期:2011-08-13 发布日期:2011-08-13
  • 作者简介:黄俊杰★，男，1970年生，广西壮族自治区平果县人，壮族，2007年广西医科大学毕业，硕士，副教授，主要从事神经生理与神经系统疾病方面的研究。 moke68@163. com
  • 基金资助:

    广西教育厅科研项目，桂教科研[2007]34号(200707LX136)，项目名称：bFGF对慢性衰老模型大鼠神经细胞保护作用的研究。

Effects of modified acidic fibroblast growth factor on free radical and lipid peroxide in aging model rats

Huang Jun-jie1, Wang Cai-bing1, He Xian-jiao1, Huang Li-juan1, Huang Yan-feng1, Zhao Shan-min1, Li Qian-ming2, Huang Ju-en3   

  1. 1Department of Applied Physiology, Youjiang Medial College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China; 2Department of Physiology, 3Department of Histology and Embryology, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-09 Revised:2011-07-09 Online:2011-08-13 Published:2011-08-13
  • About author:Huang Jun-jie★, Master, Associate professor, Department of Applied Physiology, Youjiang Medial College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China moke68@163.com
  • Supported by:

    Scientific Research Program of Education Department of Guangxi Zhuang Autonomous Region, No.Guikejiaoyan[2007]34(200707LX136)*

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284

被引次数

2

摘要：

背景：有研究表明改构型酸性成纤维细胞生长因子是多功能生长因子，但其抗衰老作用至今尚未见报道。
目的：观察改构型酸性成纤维细胞生长因子对D-半乳糖致衰老大鼠脑组织、肝组织及血清中超氧化物歧化酶活力、丙二醛含量和抑制羟自由基能力的影响。
方法：选择成年Wistar大鼠皮下注射D-半乳糖建立衰老模型，建模成功后随机分为模型组、生理盐水对照组和改构型酸性成纤维细胞生长因子治疗组，另设正常对照组。改构型酸性成纤维细胞生长因子组按12 µg/kg剂量肌肉注射改构型酸性成纤维细胞生长因子，生理盐水对照组肌肉注射等量的生理盐水，模型组不作干预。
结果与结论：与模型组和生理盐水对照组相比，改构型酸性成纤维细胞生长因子治疗组脑组织、肝组织及血清中超氧化物歧化酶活力和抑制羟自由基能力均显著升高(P < 0.01或P < 0.05)，丙二醛含量均显著降低(P < 0.01或P < 0.05)。结果证实，改构型酸性成纤维细胞生长因子可通过降低自由基，提高机体的抗氧化能力来发挥延缓衰老的作用。

关键词: 改构型酸性成纤维细胞生长因子, 衰老, D-半乳糖, 自由基, 超氧化物歧化酶, 丙二醛, 组织构建

Abstract:

BACKGROUND: Several studies have demonstrated that modified acidic fibroblast growth factor (maFGF) is a multifunctional growth factor, but its anti-aging effects have not been reported.
OBJECTIVE: To investigate the effects of maFGF on superoxide dismutase (SOD), malondialdehyde (MDA) and hydroxyl radical ability in cerebral cortex, liver tissue and serum of subacute aging model rats.
METHODS: Adult Wistar rats were established into aging models by subcutaneous injection of D-galactose. Then aging rat models were assigned to three groups: model, saline control, maFGF. A normal control group was set. The maFGF group rats were intramuscularly injected with 12 µg/kg maFGF. The saline control group was intramuscularly injected with the same amount of saline. The model group rats did not receive any intervention.
RESULTS AND CONCLUSION: Compared with model and saline control groups, SOD activity and hydroxyl radical ability in cerebral cortex, liver tissue and serum were significantly increased (P < 0.01 or P < 0.05), and MDA level was significantly decreased (P < 0.01 or P < 0.05) in the maFGF group. Results showed that maFGF decreases free radial level, enhances organism’s anti-aging ability, and thereby exhibiting anti-aging effects.

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