中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (11): 1957-1960.doi: 10.3969/j.issn.1673-8225.2011.11.014

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

创伤性深静脉血栓形成大鼠模型Wnt信号通路的作用

莫建文1，胡海澜2，白  波1，李  颖1，张姝江1，陈  艺1   

  1. 1广州医学院第一附属医院骨科，广东省广州市510120
    2广州医学院第二附属医院骨科，广东省广州市510260
  • 收稿日期:2010-09-30 修回日期:2010-12-29 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 白波，博士，教授，主任医师，广州医学院第一附属医院骨科，广东省广州市510120 drbobai@yahoo.com
  • 作者简介:莫建文☆，男，1978年生，广东省肇庆市人，汉族，博士，主治医师，主要从事创伤后深静脉血栓形成的基础与临床研究。 mjw1997@126. com
  • 基金资助:

    广东省自然科学基金面上项目资助(9151018201000035)，蛋白质组学研究对创伤性深静脉血栓形成早期诊断的价值。

Influence of Wnt signaling pathway in a rat model of traumatic deep vein thrombosis

Mo Jian-wen1, Hu Hai-lan2, Bai Bo1, Li Ying1, Zhang Shu-jiang1, Chen Yi1   

  1. 1Department of Orthopaedics, the First Affiliated Hospital of Guangzhou Medical College, Guangzhou  510120, Guangdong Province, China
    2Department of Orthopaedics, the Second Hospital of Guangzhou Medical College, Guangzhou  510260, Guangdong Province, China
  • Received:2010-09-30 Revised:2010-12-29 Online:2011-03-12 Published:2011-03-12
  • Contact: Bai Bo, Doctor, Professor, Chief physician, Department of Orthopaedics, the First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, Guangdong Province, China drbobai@yahoo.com
  • About author:Mo Jian-wen☆, Doctor, Attending physician, Department of Orthopaedics, the First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, Guangdong Province, China mjw1997@126.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 9151018201000035*

PDF

361

被引次数

2

摘要：

背景：创伤后深静脉血栓形成具有潜在的临床危害性，可并发肺栓塞、脑栓塞。Wnt信号途径调节控制多种疾病过程，可能影响深静脉血栓的形成。
目的：探讨Wnt信号通路在创伤性深静脉血栓形成中的作用。
方法：将30只SD大鼠随机分为正常对照组和模型组。模型组根据造模后的不同生物学状态再分为高峰期血栓形成组和高峰期血栓不形成组，造模后5d无创切取股静脉血管组织，随后抽取各组大鼠总RNA，用Genechip Rat Genome 230 2.0芯片测定股静脉RNA表达，并分析Wnt信号通路基因表达变化情况。
结果与结论：与正常对照组比较，血栓形成组发现1 906个基因出现表达差异，无血栓形成组差异表达基因数目合计1 568个；与无血栓形成组比较，血栓形成组有437个出现表达差异，其中Wnt信号通路卷曲相关蛋基因、膜联结合蛋白基因、酪蛋白激酶Ⅱ基因、p53基因、蛋白磷酶2A 基因、环腺苷酸依赖性激酶同功酶基因、连接素β基因、原癌基因、fos相关抗原基因、Rac基因、钙调素依赖型蛋白激酶Ⅱ基因、钙调神经磷酸酶基因等均上调；卷曲蛋白基因、磷脂酶C基因等下调。提示Wnt信号通路可能是调控血栓的生物学状态的重要信号通路之一。

关键词: 创伤, 并发症, 高危因素, 深静脉血栓形成, Wnt信号通路, 基因芯片

Abstract:

BACKGROUND: The potential harmful of traumatic deep vein thrombosis is great. It can be complicated by pulmonary embolism or cerebral embolism. Wnt signaling pathway controls the course of many diseases, which may affect the formation of deep vein thrombosis.
OBJECTIVE: To study the role of Wnt signaling pathway in traumatic deep vein thrombosis.
METHODS: Totally 30 SD rats were divided into control and model groups. The model group was divided into 2 groups according to biological states: the crest-time of thrombosis and without thrombosis at the crest-time. Femoral vein tissues were incised at 5 days after model preparation, and total RNA was extracted. The RNA expression was determined by Genechip Rat genome 230 2.0 genechips and the changes of Wnt signaling pathway signaling pathway was analyzed. 
RESULTS AND CONCLUSION: Compared with the control group, 1 906 genes were differential expressed in the thrombosis, and 1 568 genes were differential expressed in the non-thrombosis group; compared with the non-thrombosis group, 43 genes were differential expressed in the thrombosis group, including Wnt signaling pathway of frizzled-related protein, Axam, CK2, P53, PP2A, PKA,β-catenin, c-myc, fra-1, Rac, calcium/calmodulin dependent kinase II and calcineurin were up regulated, frizzled-related protein and phospholipase C were down regulated. Wnt signaling pathway may be one of the most important pathway to control the biological states of thrombosis.

中图分类号: