中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (2): 286-289.doi: 10.3969/j.issn.1673-8225.2011.02.023

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

胎儿和成人皮肤相关基因的差异表达

吴志远1，郭晓瑞1，黄海华1，段红梅2，彭  智1，梁  杰1   

  1. 1广东医学院附属医院整形外科，广东省湛江市  524001
    2佛山市顺德区北滘医院门诊外科，广东省佛山市  528300
  • 收稿日期:2010-07-23 修回日期:2010-09-25 出版日期:2011-01-08 发布日期:2011-01-08
  • 通讯作者: 郭晓瑞，广东医学院在读硕士，医师，广东医学院附属医院整形外科，广东省湛江市 524001 guoxiaorui830405@163.com
  • 作者简介:吴志远★，男，1970年生，广东省湛江市人，汉族，2001年广东医学院毕业，硕士，副主任医师，主要从事病理性瘢痕、难愈性创面及糖尿病足的基础和临床研究。 wuzhiyuan1388@yahoo.com.cn
  • 基金资助:

    湛江市科技计划项目(2008C01003)，课题名称：胎儿和成人皮肤相关基因表达谱的研究。

Differentially expressed genes in fetal and adult skins

Wu Zhi-yuan1, Guo Xiao-rui1, Huang Hai-hua1, Duan Hong-mei2, Peng Zhi1, Liang Jie1   

  1. 1Department of Plastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China
    2Department of Ambulatory Surgery, Shunde District Beijiao Hospital of Foshan City, Foshan  528300, Guangdong Province, China
  • Received:2010-07-23 Revised:2010-09-25 Online:2011-01-08 Published:2011-01-08
  • Contact: Guo Xiao-rui, Studying for master’s degree, Physician, Department of Plastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China guoxiaorui830405@163.com
  • About author:Wu Zhi-yuan★, Master, Associate chief physician, Department of Plastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China wuzhiyuan1388@yahoo.com.cn
  • Supported by:

    the Science and Technology Plan of Zhanjiang City, No. 2008C01003

PDF

309

被引次数

8

摘要：

背景：胎儿皮肤创伤后无瘢痕愈合是胎儿发育过程特定阶段的特殊现象，其机制目前尚未明确，可能与调控基因的表达有关。
目的：运用人类基因芯片技术，研究人胎儿及成人正常皮肤差异表达基因及其特征和可能的生物学意义。
方法：选择人胎儿(孕20~24周)皮肤和成人(18~48岁)皮肤各10例，提取各标本的总DNA与RNA，制成荧光标记的cDNA探针，与含10 724个人类靶基因的芯片杂交，经扫描、生物信息学分析，比较两种组织基因的差异表达。
结果与结论：基因芯片高通量地揭示了胎儿及成人正常皮肤基因信息的差异表达。与成人正常皮肤比较，胎儿皮肤基因显著差异表达83个，已明确功能基因26个，涉及多种信号传递及基因调控的改变。说明胎儿与成人正常皮肤差异表达基因的存在在一定程度上导致了胎儿创伤的无瘢痕愈合。

关键词: 胎儿, 成人, 皮肤, 基因芯片, 基因表达

Abstract:

BACKGROUND: It is the specific phenomenon that scarless fetal skin wound healing after a certain stage of fetal development process, and its mechanism is not yet clear. Research suggests that the fetus in a specific state of developmental biology may be the root cause of this phenomenon.
OBJECTIVE: To study human fetal and adult skin of differentially expressed genes and their characteristics and possible biological significance using gene microarray.
METHODS: The total DNA and RNA from fetus (gestational age 20-24 weeks) skin and adults (18-48 years old) normal skins were isolated and purified to mRNA by oligotex. They were reversely transcribed to cDNA with the incorporation of fluorescent dTUP to prepare the hybridization probes. Then, the mixed probes were hybridized to the cDNA chip and scanned for the signals and found differences between fetal skin and adult normal skin.
RESULTS AND CONCLUSION: Gene chips high-throughput revealed the differential expression of genetic information of the fetal and adult skins. Compared gene expression in normal adult skin, fetal skin, and significant differences in gene expression of 83 genes had clear functions in 26, involving a variety of signal transduction and gene regulation changes. Wound healing lead to excessive scar is a complex pathophysiological process, the presence of genes’ differences between fetal and adult normal skin may lead to fetal wounds heal without scar formation, help to understand the mechanism of the formation of abnormal scars.

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