中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9249-.doi: 10.3969/j.issn.1673-8225.2010.49.029

• 干细胞基础实验 • 上一篇    下一篇

体外心脏震波治疗前后外周血内皮祖细胞数量的变化

蔡红雁，王钰，李琳，赵玲，肖践明，曹晶茗，杨西云，杨超，郭涛   

  1. 昆明医学院第一附属医院心内科，云南省昆明市 650031
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: 郭涛，硕士，主任医师，博士生导师，昆明医学院第一附属医院心内科，云南省昆明市 650031 GuoTao20@hotmail.com
  • 作者简介:蔡红雁☆，女，1971年生，汉族，昆明医学院在读博士，主治医师，主要从事冠状动脉粥样硬化性心脏病基础及介入研究。 hyflykm@sina.com.cn
  • 基金资助:

    云南省社会攻关项目(2006SG10)，《经胸心脏超声震波治疗复杂冠心病应用研究》；云南省社会发展计划项目(2008CD007)，《体外心脏震波治疗对猪急性心肌梗死后心室重塑影响的研究》。

Changes in the number of endothelial progenitor cells from peripheral blood in patients with coronary atherosclerotic heart disease before and after extracorporeal cardiac shock wave therapy

Cai Hong-yan, Wang Yu, Li Lin, Zhao Ling, Xiao Jian-ming, Cao Jing-ming, Yang Xi-yun, Yang Chao, Guo Tao   

  1. Department of Cardiology, the First Affiliated Hospital of Kunming Medical College, Kunming    650031, Yunnan Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Guo Tao, Master, Chief physician, Doctoral supervisor, Department of Cardiology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China GuoTao20@hotmail.com
  • About author:Cai Hong-yan☆, Studying for doctorate, Attending physician, Department of Cardiology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China hyflykm@sina.com.cn
  • Supported by:

    Social Research Project of Yunnan Province, No.2006SG10*; Social Development Program of Yunnan Province, No.2008CD007*

PDF

412

被引次数

13

摘要：

背景：体外心脏震波治疗对冠状动脉粥样硬化性心脏病患者外周血内皮祖细胞影响的机制，目前尚不清楚。
目的：观察体外心脏震波治疗对冠状动脉粥样硬化性心脏病患者外周血内皮祖细胞增殖、分化的影响。
方法：选择行体外心脏震波治疗冠状动脉粥样硬化性心脏病患者25例。以未行心脏震波治疗冠状动脉粥样硬化性心脏病患者10例作为对照组，从外周血获取单个核细胞，将其在EGM-2-MV培养基培养，并分析细胞形态和形成集落的数量，7 d后贴壁细胞进行细胞分析和计数。用激光共聚焦显微镜鉴定分化的内皮祖细胞。
结果与结论：震波治疗组患者外周血内皮祖细胞数量、形成细胞集落数较对照组明显增多(P < 0. 05)，提示体外心脏震波治疗对冠状动脉粥样硬化性心脏病患者外周血内皮祖细胞增殖及向内皮细胞系分化有显著促进作用。

关键词: 体外心脏震波, 内皮祖细胞, 增殖, 分化, 冠状动脉粥样硬化性心脏病, 组织构建, 组织工程

Abstract:

BACKGROUND: The mechanism underlying the effect of extracorporeal cardiac shock wave therapy on endothelial progenitor cells (EPCs) from peripheral blood in patients with coronary atherosclerotic heart disease (CAHD) is poorly understood.
OBJECTIVE: To observe the effects of cardiac shock wave therapy on the proliferation and the differentiation of EPCs from peripheral blood in CAHD patients.
METHODS: A total of 25 CAHD patients undergoing extracorporeal cardiac shock wave therapy were selected, and another 10 patients who did not receive extracorporeal cardiac shock wave therapy served as controls. Mononuclear cells obtained from peripheral blood were cultured in EGM-2-MV medium. Cell morphology and the number of colonies formed were observed and analyzed. After 7 days of culture, adherent cells were analyzed and counted. Whether EPCs differentiated or not was identified by laser confocal microscopy.
RESULTS AND CONCLUSION: The number of EPCs from peripheral blood and colonies formed were significantly increased (P < 0.05) compared with the control group. It is indicated that extracorporeal cardiac shock wave therapy can promote the proliferation and differentiation of EPCs from peripheral blood into endothelial cell lineage in CAHD patients.

中图分类号: