中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9167-.doi: 10.3969/j.issn.1673-8225.2010.49.011

• 干细胞移植 • 上一篇    下一篇

嗅鞘细胞移植损伤脊髓组织的超微结构变化

刘晓阳，孙建民，崔新刚，蒋振松   

  1. 山东大学附属省立医院脊柱外科，山东省济南市 250021
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: 通讯作者：孙建民，博士，主任医师，山东大学附属省立医院脊柱外科，山东省济南市 250021 spine163@163.com
  • 作者简介:刘晓阳★，男，1984年生，山东省青州市人，汉族，在读硕士，主要从事脊髓损伤修复的研究。 shuilanwenqing@163.com
  • 基金资助:

    山东省自然科学基金资助项目(Y2007C010)。课题名称：VEGF基因转染联合嗅鞘细胞移植对脊髓损伤再生修复作用的实验研究。

Ultrastructure of injured spinal cord following olfactory ensheathing cells transplantation

Liu Xiao-yang, Sun Jian-min, Cui Xin-gang, Jiang Zhen-song   

  1. Department of Spinal Surgery, Provincial Hospital Affiliated to Shandong Univeristy, Jinan  250021, Shandong Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Sun Jian-min, Doctor, Chief physician, Department of Spinal Surgery, Provincial Hospital Affiliated to Shandong Univeristy, Jinan 250021, Shandong Province, China spine163@163.com
  • About author:Liu Xiao-yang, Studying for master’s degree, Department of Spinal Surgery, Provincial Hospital Affiliated to Shandong Univeristy, Jinan 250021, Shandong Province, China shuilanwenqing@163.com
  • Supported by:

    the Natural Science Foundation of Shandong Province, No. Y2007C010

PDF

358

被引次数

1

摘要：

背景：脊髓损伤后神经功能难以自行恢复，嗅鞘细胞具有外周性和中枢性两种胶质细胞的成鞘功能，是修复受损神经最有前途的种子细胞。嗅鞘细胞移植到受损脊髓后的组织学和超微结构的变化可能帮助解释嗅鞘细胞发挥修复作用的机制。
目的: 验证嗅球源性嗅鞘细胞移植对脊髓损伤功能恢复的促进作用，并观察移植的嗅鞘细胞对神经元和轴突组织和超微结构的影响。
方法：将已制备脊髓模型的Wistar大鼠随机分为3组，对照组不做任何注射操作，DMEM/F12组注射DMEM/F12培养基，嗅鞘细胞组注射嗅鞘细胞悬液。每周进行肢体活动BBB评分，8周后取脊髓标本进行组织学和免疫组织化学观察，评价脊髓损伤的修复情况，并观察嗅鞘细胞移植对脊髓组织和超微结构的影响。
结果与结论：3组动物均出现后肢运动功能的恢复，嗅鞘细胞组优于对照组和DMEM/F12组，在4周后更为明显。组织学观察可见，在嗅鞘细胞组可见有神经纤维通过损伤处。损伤处附近，嗅鞘细胞组脊髓腹侧的神经纤维和神经元形态较好，损伤较轻。而对照组和DMEM/F12组神经纤维和神经元损害严重。嗅鞘细胞组的caspsase-3阳性细胞数少于对照组和DMEM/F12组。超微结构观察可见，嗅鞘细胞组的神经纤维和细胞形态均优于对照组和DMEM/F12组。结果表明嗅鞘细胞移植对大鼠脊髓损伤修复有明显的促进作用，并可恢复损伤神经的部分功能，对受损神经纤维和神经元有保护作用。

关键词: 嗅鞘细胞, 大鼠, 移植, 脊髓损伤, 组织, 超微结构

Abstract:

BACKGROUND: Neural function automatically recovers a little after spinal cord injury. Because of the capacity of myelination belonging to both peripheral and central glial cells, olfactory ensheathing cells (OECs) become the most promising cells for repairing damaged nerves. The histological and ultrastructural changes of spinal cord after OECs grafting may help to explain the mechanism of neural restoration.
OBJECTIVE: To assess the effect of transplantation of OECs originating from the olfactory bulbs to repair spinal cord injury, and explore the influence of grafted OECs on histology and ultrastructure of neurons and axons.
METHODS: Wistar rat models were randomly divided into three groups: the control group (group A, no treatment), DMEM/F12 group (group B, administrated with DMEM/F12 medium) and the OECs group (group C, treated with OEC suspension). The Basso, Beattie and Bresnahan locomotor rating scale (BBB) was observed each week. Histological changes and immunohistochemical staining were observed 8 weeks later. Reparation of spinal cord injury was assessed. Effects of OEC transplantation on spinal cord tissue and ultrastructure were observed.
RESULTS AND CONCLUSION: The posterior limb motor function was recovered in three groups. The BBB grade of groups    C was higher than that of group A and group B, especially in the last 4 weeks post operation. Histology revealed that both stumps were connected by nerve fibers through the injury site in group C. Less damage of nerve fibers and neurons at the vicinity of damage site was observed in group C than that in group A and group B. The percentage of caspsase-3 positive cells in group   C was smaller than that in group A and group B. The ultrastructure showed that morphology of fiber and neuron in group C was better than that in groups A and B. Results suggest that transplantation of OECs has significant promotory effects on restoration of spinal cord injury in the rat, and can improve part function of nerve fibers and shows neuroprotection on neurons and nerve fibers.

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