中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9172-.doi: 10.3969/j.issn.1673-8225.2010.49.012

• 干细胞移植 • 上一篇    下一篇

腰椎穿刺移植自体骨髓间充质干细胞治疗脑梗死后遗症6例

焦红亮1，2，关方霞3，杨波1，李建斌2，詹三华4，杜英5，胡祥6，赵琳娜2，韩小改2   

  1. 1郑州大学第一附属医院神经外科，河南省郑州市 450052；2 河南省红十字血液中心，河南省郑州市  450014；3郑州大学生物医学工程学系，河南省郑州市450052；4河南省武警总队医院普内科，河南省郑州市  450052；  5郑州大学基础医学院微生物与免疫学教研室，河南省郑州市
    450052；6深圳市北科生物科技有限公司，广东省深圳市 518000
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: 杨波，博士，教授，博士生导师，郑州大学第一附属医院神经外科，河南省郑州市 450052 yangbo96@126.com
  • 作者简介:焦红亮，男，1978年出生，河南省郑州市人，2008年郑州大学毕业，主要从事干细胞基础和临床应用方面的研究。 jhl787878@126.com
  • 基金资助:

    郑州大学211工程三期重点学科建设项目;河南省医学科技创新人才工程项目(2005018)。

Autologous bone marrow mesenchymal stem cell transplantation via lumbar puncture in the treatment of cerebral infarction sequelae in 6 cases

Jiao Hong-liang 1,2 , Guan Fang-xia 3, Yang Bo1, Li Jian-bin2, Zhan San-hua4, Du Ying5, Hu Xiang6, Zhao Lin-na 2, Han Xiao-gai2   

  1. 1 Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 2 Henan Province Red Cross Blood Center, Zhengzhou  450014, Henan Province, China; 3 Department of Biomedical Engineering, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 4 Department of General Internal Medicine, Henan Province Armed Police Forces Hospital, Zhengzhou  450052, Henan Province, China; 5 Department of Microorganism and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 6 Beike Bio-Technology Co., Ltd., Shenzhen  518000, Guangdong Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Yang Bo, Doctor, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Jiao Hong-liang, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Henan Province Red Cross Blood Center, Zhengzhou 450014, Henan Province, China jhl787878@126.com
  • Supported by:

    the Key Subject Construction Project of 211 Engineering of Zhengzhou University*; the Medical Science Technology Innovation Talent Engineering Program of Henan Province, No. 2005018*

PDF

374

被引次数

5

摘要：

背景：骨髓间充质干细胞具有多向分化潜能，而且取材方便，扩增迅速，免疫原性低，在移植过程避免了免疫排斥等反应，是治疗脑梗死等神经系统疾病的理想种子细胞。
目的：探讨自体骨髓间充质干细胞培养后经腰穿治疗脑梗死后遗症的安全性、可行性及疗效。
方法：6例脑梗死后遗症患者进行自体骨髓间充质干细胞移植，骨穿采集自体骨髓单个核细胞，体外分离培养骨髓间充质干细胞4 d后经腰椎穿刺移植蛛网膜下腔，移植后观察疗效及并发症。
结果与结论：培养后骨髓间充质干细胞数量明显增多，患者手术前后肌力和NIHSS评分通过统计分析有显著性差异(P < 0.05)，总体症状改善较术前有明显改善；移植后无严重不良事件发生。结果提示，自体骨髓间充质干细胞培养后移植治疗可使脑梗死后遗症患者症状明显改善，治疗安全有效且不良反应小。

关键词: 脑梗死后遗症, 骨髓间充质干细胞, 培养, 移植, 腰椎穿刺

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells have multi-directional differentiation potential, and are easily, rapidly expanded, low immunogenicity, avoid immunological rejection in the transplant process, are ideal seed cells in the treatment of neurological diseases such as cerebral infarction.
OBJECTIVE: To explore the safety, feasibility and efficacy of autologous bone marrow mesenchymal stem cell after culture transplantation via lumbar puncture in treatment of cerebral infarction.
METHODS: A total of 6 cases of sequelae of cerebral infarction received autologous bone marrow mesenchymal stem cell transplantation. Autologous bone marrow mononuclear cells were collected by bone puncture. Bone marrow mesenchymal stem cells in vitro cultured 4 days after transplantation by lumbar puncture in the subarachnoid space. The efficacy and complications were observed after transplantation.
RESULTS AND CONCLUSION: Numbers of BMSCs were significantly increased after culture. There were significant differences in NIHSS scores and muscle strength of patients before and after operation (P < 0.05) and the overall improvement of symptoms were improved significantly. After transplantation, there were no serious adverse events. The results suggest that autologous bone marrow mesenchymal stem cell transplantation can significantly improve symptoms in patients with cerebral infarction sequelae; treatment is safe, effective and low side effects.

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