中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7639-7642.doi: 10.3969/j.issn.1673-8225.2010.41.010

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

组织工程生物反应器嵌入式实时检测控制系统的设计

刘圹彬1，黄  华1，曾  军2，刘  怡3   

  1. 四川大学电气信息学院，1医学信息工程系； 2信号与信息处理，四川省成都市  610065；  3西南技术物理研究所，四川省成都市  610041
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 黄华，博士(后) ，教授，博士生导师，四川大学医学信息工程系，四川省成都市 610065 hhua@scu.edu.cn
  • 作者简介:刘圹彬★，男，1985年生，四川省自贡市人，汉族，四川大学在读硕士，主要从事医学仪器与医学信号处理研究。 cairmy@163.com
  • 基金资助:

    四川省应用基础研究项目(07JY029)，智能组织工程生物反应器系统；四川大学创新基金项目(2005CF14)，智能组织工程生物反应器。

Design of an embedded real-time detecting and controlling system for tissue engineered bioreactor

Liu Kuang-bin1, Huang Hua1, Zeng Jun2, Liu Yi3   

  1. 1 Department of Medical Information Engineering, 2 Department of Signal and Information Processing, School of Electrical Engineering and Information, Sichuan University, Chengdu  610065, Sichuan Province, China; 3 Southwest Institute of Technical Physics, Chengdu  610041, Sichuan Province, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Huang Hua, Doctor, Professor, Doctoral supervisor, Department of Medical Information Engineering, School of Electrical Engineering and Information, Sichuan University, Chengdu 610065, Sichuan Province, China hhua@scu.edu.cn
  • About author:Liu Kuang-bin★, Studying for master’s degree, Department of Medical Information Engineering, School of Electrical Engineering and Information, Sichuan University, Chengdu 610065, Sichuan Province, China cairmy@163.com
  • Supported by:

     the Applied Basic Research of Sichuan Province, No. 07JY029*; the Innovation Foundation of Sichuan University, No. 2005CF14*

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被引次数

1

摘要：

背景：组织工程生物反应器通过模拟体内环境，可为细胞或组织提供适宜的生长条件，并能培养出与体内结构和功能相似的三维细胞或组织。
目的：对水平旋转生物反应器培养细胞或组织的环境需求进行分析，针对其特殊的环境需求提出了基于Linux和ARM9嵌入式处理器的生物反应器检测控制系统设计。
方法：以系统对实验室自制的生物反应器进行控制监测。实验主要完成支架材料制备，实验用主要仪器(自动台式灭菌器，气液膜，液液膜电子分析天平等)准备，实验材料-骨髓间充质干细胞的原代、传代培养及制备，以及系统对反应器的转速和蠕动泵控制，温度，pO2，pH值的检测。
结果与结论：与单片机控制检测系统相比，改进系统驱动控制方式，提高了控制精度，增强检测灵敏度。同时新设计增加了养分压以及pH值检测，进一步完善了反应器的功能。

关键词: 水平旋转生物反应器, 嵌入式处理器, Linux系统, 检测控制, 组织构建

Abstract:

BACKGROUND: Tissue engineering bioreactor is used to simulate the cell’s metabolism in vivo and provide a condition to culture the three dimensional cells and tissue in vitro.
OBJECTIVE: To analyze the bioreactor’s needs for culturing and design a detecting and controlling system based on Linux and ARM9 embedded processor for the bioreactor.
METHODS: The system was used to control and detect the bioreactor made by our laboratory. The experiment contained preparing the scaffold and devices (Auto desktop sterilizer, gas-liquid membrane, gas-gas membrane and so on), bone marrow mesenchymal stem cells cultivation, the controlling of bioreactor, as well as detections of temperature, oxygen partial pressure, and pH value.
RESULTS AND CONCLUSION: Compared with the system based on MCU, the new design improved the driving and parameter detecting and added the oxygen partial pressure and pH monitoring modules. The function of the bioreactor was perfect. 

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